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The map label for this gene is ask [H]

Identifier: 197104307

GI number: 197104307

Start: 928203

End: 929450

Strand: Reverse

Name: ask [H]

Synonym: PHZ_c0841

Alternate gene names: 197104307

Gene position: 929450-928203 (Counterclockwise)

Preceding gene: 197104310

Following gene: 197104305

Centisome position: 23.26

GC content: 69.79

Gene sequence:

>1248_bases
ATGTCCCGGCTGGTGATGAAATTCGGCGGCACCTCGGTGGCCGACCTGGAGCGGATCCGCCGGGTGGCGCGGCTGGTGGC
CGCCGAGATCGAGGCGGGCCATCAGGTGGCGGTCGTCGTCTCGGCCATGGCCGGCAAGACCAACGAGCTGGTCGCGTGGA
CCGACGGGGCCGGCGCCGCGGCCCAGGGGATCGACCCCTCGGACGACGAGTACGACGTTGTGGTGGCCTCGGGCGAGCAG
GTGACCGCGGGCCTCCTGGCCATGACGCTGCGCAACATGGGCCTGCGGGCCAAGTCGTGGCTGGGCTGGCAGGTGCCGAT
CCTCACCGACGAGGCGCACGGCAAGGCGCGGATCGACGAGATTCCGCCTGAGAAGCTGTCGGCGGCGCTGGACGCCGGCG
AGGTGGCGGTGGTCGCCGGCTTCCAGGGCGTGACCCGCGACGGCCGCATCGCCACCCTGGGCCGCGGCGGCTCGGACACC
AGCGCCGTGGCGATCGCCGCCTCGCTGGGCGCAGCCTGCGACATCTACACCGACGTGGACGGGGTCTACACCACCGACCC
GCGGATCGAATCCAAGGCCAGGAAGCTCGCGAAGGTCTCCTACGAGGAGATGCTCGAGATGGCCTCGCTGGGCGCCAAGG
TCCTGCAGACCCGCTCGGTCGAGCTGGCCATGGCCTACCATGTGCCCGTCAGGGTGCTTTCCAGTTTTGTCGAGCCCGGC
GAAGCGCCCGGCCAGGGCACTATCGTCTGCGACGAGGAGGAGATCGTGGAGAAGCGGATCGTGAGCGGCGTGGCCTACAG
CCGCGACGAGGCGAAGATCAGCCTCTTCGGCCTGCCCGACCATCCGGGCGTGTCGTCCACCATCTTCGGCGCGCTGGCCG
ATGCGAACGTGAACGTGGACATGATCGTCCAGAGCCACGCGCGCACCGCCGACACCGCCAACATGGAGTTCACCGTCGGC
CGCCGGGACGCCCAGCGGGCGGTGGAGATCATCCGCGCCGTACAGCCGCAGGTCGGCTTCGAGGACGTCCGCGTGGACGA
GGACGTGGCCAAGGTCTCGGTCATCGGCGTGGGCATGCGCAGCCACGCCGGCGTCGCCAAGTCGATGTTCAAGGCGCTGG
CCGACAAGGGCGTGAACATCCAGGTGATCTCGACGTCCGAGATCAAGATCAGCGTGCTGATCGACGCGGCCTACACCGAG
CTGGCCGTGCGCGCCCTGCACGCGGCCTACGGGCTGGACCAGCTCTAG

Upstream 100 bases:

>100_bases
CGAAACGAAAGATTCTGAGCGTTGCCGCAGGCGGCGCGCGCCGCTATGAGGCGACGCCCTTTCGCGGCGCAGCAAGCGAC
AAAGGTGGTCCTGGCGTTCG

Downstream 100 bases:

>100_bases
GCCTTCTCCGCGCCGCCGGTCAGGCGGAAGGCGGCCCAGGTTCCGGCCAGGGCCAGGGGGATCACGGCCACGAACACCCA
GACCGAGGCGGCGCGGGCGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSRLVMKFGGTSVADLERIRRVARLVAAEIEAGHQVAVVVSAMAGKTNELVAWTDGAGAAAQGIDPSDDEYDVVVASGEQ
VTAGLLAMTLRNMGLRAKSWLGWQVPILTDEAHGKARIDEIPPEKLSAALDAGEVAVVAGFQGVTRDGRIATLGRGGSDT
SAVAIAASLGAACDIYTDVDGVYTTDPRIESKARKLAKVSYEEMLEMASLGAKVLQTRSVELAMAYHVPVRVLSSFVEPG
EAPGQGTIVCDEEEIVEKRIVSGVAYSRDEAKISLFGLPDHPGVSSTIFGALADANVNVDMIVQSHARTADTANMEFTVG
RRDAQRAVEIIRAVQPQVGFEDVRVDEDVAKVSVIGVGMRSHAGVAKSMFKALADKGVNIQVISTSEIKISVLIDAAYTE
LAVRALHAAYGLDQL

Sequences:

>Translated_415_residues
MSRLVMKFGGTSVADLERIRRVARLVAAEIEAGHQVAVVVSAMAGKTNELVAWTDGAGAAAQGIDPSDDEYDVVVASGEQ
VTAGLLAMTLRNMGLRAKSWLGWQVPILTDEAHGKARIDEIPPEKLSAALDAGEVAVVAGFQGVTRDGRIATLGRGGSDT
SAVAIAASLGAACDIYTDVDGVYTTDPRIESKARKLAKVSYEEMLEMASLGAKVLQTRSVELAMAYHVPVRVLSSFVEPG
EAPGQGTIVCDEEEIVEKRIVSGVAYSRDEAKISLFGLPDHPGVSSTIFGALADANVNVDMIVQSHARTADTANMEFTVG
RRDAQRAVEIIRAVQPQVGFEDVRVDEDVAKVSVIGVGMRSHAGVAKSMFKALADKGVNIQVISTSEIKISVLIDAAYTE
LAVRALHAAYGLDQL
>Mature_414_residues
SRLVMKFGGTSVADLERIRRVARLVAAEIEAGHQVAVVVSAMAGKTNELVAWTDGAGAAAQGIDPSDDEYDVVVASGEQV
TAGLLAMTLRNMGLRAKSWLGWQVPILTDEAHGKARIDEIPPEKLSAALDAGEVAVVAGFQGVTRDGRIATLGRGGSDTS
AVAIAASLGAACDIYTDVDGVYTTDPRIESKARKLAKVSYEEMLEMASLGAKVLQTRSVELAMAYHVPVRVLSSFVEPGE
APGQGTIVCDEEEIVEKRIVSGVAYSRDEAKISLFGLPDHPGVSSTIFGALADANVNVDMIVQSHARTADTANMEFTVGR
RDAQRAVEIIRAVQPQVGFEDVRVDEDVAKVSVIGVGMRSHAGVAKSMFKALADKGVNIQVISTSEIKISVLIDAAYTEL
AVRALHAAYGLDQL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=483, Percent_Identity=29.1925465838509, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=29.2841648590022, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790376, Length=463, Percent_Identity=25.0539956803456, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320893, Length=374, Percent_Identity=25.668449197861, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43844; Mature: 43713

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVMKFGGTSVADLERIRRVARLVAAEIEAGHQVAVVVSAMAGKTNELVAWTDGAGAA
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCEEEEECCCCCC
AQGIDPSDDEYDVVVASGEQVTAGLLAMTLRNMGLRAKSWLGWQVPILTDEAHGKARIDE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCC
IPPEKLSAALDAGEVAVVAGFQGVTRDGRIATLGRGGSDTSAVAIAASLGAACDIYTDVD
CCHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHEEHHHHHCCEEEEEECCC
GVYTTDPRIESKARKLAKVSYEEMLEMASLGAKVLQTRSVELAMAYHVPVRVLSSFVEPG
CEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCC
EAPGQGTIVCDEEEIVEKRIVSGVAYSRDEAKISLFGLPDHPGVSSTIFGALADANVNVD
CCCCCCCEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEE
MIVQSHARTADTANMEFTVGRRDAQRAVEIIRAVQPQVGFEDVRVDEDVAKVSVIGVGMR
EEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCH
SHAGVAKSMFKALADKGVNIQVISTSEIKISVLIDAAYTELAVRALHAAYGLDQL
HCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEEHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLVMKFGGTSVADLERIRRVARLVAAEIEAGHQVAVVVSAMAGKTNELVAWTDGAGAA
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCEEEEECCCCCC
AQGIDPSDDEYDVVVASGEQVTAGLLAMTLRNMGLRAKSWLGWQVPILTDEAHGKARIDE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCC
IPPEKLSAALDAGEVAVVAGFQGVTRDGRIATLGRGGSDTSAVAIAASLGAACDIYTDVD
CCHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHEEHHHHHCCEEEEEECCC
GVYTTDPRIESKARKLAKVSYEEMLEMASLGAKVLQTRSVELAMAYHVPVRVLSSFVEPG
CEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCC
EAPGQGTIVCDEEEIVEKRIVSGVAYSRDEAKISLFGLPDHPGVSSTIFGALADANVNVD
CCCCCCCEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEE
MIVQSHARTADTANMEFTVGRRDAQRAVEIIRAVQPQVGFEDVRVDEDVAKVSVIGVGMR
EEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCH
SHAGVAKSMFKALADKGVNIQVISTSEIKISVLIDAAYTELAVRALHAAYGLDQL
HCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEEHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]