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The map label for this gene is ptsI [H]

Identifier: 197104305

GI number: 197104305

Start: 924329

End: 926593

Strand: Reverse

Name: ptsI [H]

Synonym: PHZ_c0839

Alternate gene names: 197104305

Gene position: 926593-924329 (Counterclockwise)

Preceding gene: 197104307

Following gene: 197104304

Centisome position: 23.19

GC content: 72.94

Gene sequence:

>2265_bases
ATGCCGGCGCAAGGCATGGCCATTCGAGGACAGCGGAGCCTGCTTCGGCAGATCCGGGAGGCGCTGGCGGGCGGCGGCCC
GGCCCAGGCCCGTCTCGACATGGTCGTGCGGATCATCGCCCTGTCGATGGTCGCCGAGGTCTGCTCGATCTACCTGCGCC
GGGCGACCGGCGAGATGGAGCTGTTCGCCACCGAGGGCCTGGAGCCTGGCGCGGTCCACGTCACCCGCATGAAGCCCGGC
GAGGGCCTGGTCGGCGAGATCATGCGCCTGGGCCGGCCGCTGAACCTGGCCGACGCCCCCGAGCACCCGGCGTTCTCGTA
CCGCCCGGAGACGGGCGAAGACCCCTACCACGCGTTCCTGGGCGTGCCGCTGCTGCGCGGCGGGCGGGCGATCGGCGTGC
TGGTCGTCCAGAACCGCACCGCCCGGGTCTACACCGAGGACGAGGTCGAGGACCTGCAGATCATCGCCATGGTCCTGGCC
GAGATGGTGGCCTCGGGCGAGCTGATCGGCGTCGAGGAGATGAACGGGGTCGAGCTGGCGCCCCGCCGGCCCGACCGCCT
GAAGGGCTCCAGGTTCGCCGAGGGTCTGGCCTACGGCGTGGCCGTGCTGCACGAGCCGCCGGTCGCGCCCAGCCAGCTGC
TGTCGGAGGACGCCGCCGCCGAGGAGATCCGGCTCAAGGGCGCCATCGAGGCGCTGCAGGCCCAGATCGACGAGATGCTG
GAGGGCCAGCACGGCCTGGTGGACGCCTCGTACGAGGTGCTCGAGACCTACCGCATGTTCGCCCACGACCGGGGCTGGAA
CCGCAGCCTCGAGGACGCCGTGCGCAACGGCCTGACCGCCGAGGCCGCCGTCGAGCGCGTACGCTCCGAACACCGCGCGC
GCCTGGGCCAGGCGCGCGATCCCTATCTGCGCGAGCGGCTGCACGACCTGGAAGACCTGAACGACCGGCTGCTGCGCCAC
CTGGCCGGCGACGGCCACGTGGCCCGCGACCTGCCCGAGAACGCGATCCTGATCGCCCGCAACCTGGGCCCCGCGGACCT
GCTCGAGTACGACCGCACCAGGCTGCGCGGCCTGCTGCTGGAGGAGGGTTCGTCGGCCAGCCACGCGGCGATCGTGGCCC
GGGCGCTGGACATCCCCTGCGTCGGCCGCCTGCCCGGCCTGCGCGACCGGGTCTCGGAGGGCGACCCGGTGATCGTCGAC
GCCGAGACGGGCGAGGCCTACCTGCGCCCGCGGACCGACGTGGTGAAGGCCATCCAGGCCCGCATCGACGTGCGCCGACA
GCGGCAGGCCGCCTTCGCCAAGCTGCGCGAGACGCCCGCCTTCACCCGCGACGGGGTGAAGATCACCCTGCTGATGAACG
CCGGCCTCGACGTGGACCTCGAGAGCATGGCCGACGCGGGCGCCGAAGGGATCGGCCTGTTCCGCACCGAGTTCCAGTTC
ATGGTCTCGGAGGAGATGCCCCGTTTCAACGCCCAGGCGGCGCTCTACGGCCGGGTGATGGACGCGGCCGGCGACAAGCC
GGTGACCTTCCGCACCCTGGACCTGGGCGGCGACAAGGTGCTGCCGTACCTGGAGGCCGAGCGCGAGGACAACCCGGCCC
TGGGCTGGCGGGCGATCCGCATGGGCCTGGACCGCCCCGCCCTGCTGCGCCTGCAGCTGCGGGCGCTGATCGCCGCCGCC
AAGGGGCGTGCGCTGCGGATCATGTTCCCGCTGGTGGCGAGCTGCGACGAGTTCCGCGCCGCCCGCGCCCTGGTGGACAC
AGAGGTGGCCTGGGCCCTGCGGCGAGGCCGGCCGGCGCCGTCGCGCCTGGACGTCGGCGCGATGATCGAGGCGCCGTCGG
TGATCTGGCACCTCGACGCCCTGCTGCCGATGACCGACTTCGTCAGCGTCGGCACCAACGACCTGATGCAGTACCTGTTC
GCCGCCGACCGCGGGAACCCGCGGGTGGCCGACCGCTACGATCCCCTGTCGCCGCCGGCGCTGCGGGCGCTGGAGACGAT
CCAGCGGGCCTGCGCCGAGACCGGCACGCCGGTCAGCGTCTGCGGCGAGATGGCGGGCCGGCCGCTGGAGGCCTTCACCC
TGGTGGCCCTGGGCTTCGAGCGGCTGTCGATGCCGCCTGCGGGGATCGGGCCGGTGAAGCAGATGGTGCTGTCGTGCGAC
CGCGAGGCGGCCCGGCGGGGCGTTTCGGCCCTGCTGAAGTCCGGCGCGGGATCGGTGCGCAACGAGATCGAAACCTTGGC
GCGGAAACTCTATCTCGCCGTGTAG

Upstream 100 bases:

>100_bases
GCAGGCGCAGCAAACCGTCGTCGAATTGGCCTCTTCCCGTCTCGGAACGGAGAGATAAAGCTGTTAGGCAACGGGCCGAG
ACGGAGGACGCCAGGGACGG

Downstream 100 bases:

>100_bases
GCGAATCAGCCCACCGGACGTGACCGAACAACGCATTGAGCACTACCTGAACGCGTGCCACGCTCCCTACGAGTCACGAT
CCCCAGACGCGGGCGCCGGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MPAQGMAIRGQRSLLRQIREALAGGGPAQARLDMVVRIIALSMVAEVCSIYLRRATGEMELFATEGLEPGAVHVTRMKPG
EGLVGEIMRLGRPLNLADAPEHPAFSYRPETGEDPYHAFLGVPLLRGGRAIGVLVVQNRTARVYTEDEVEDLQIIAMVLA
EMVASGELIGVEEMNGVELAPRRPDRLKGSRFAEGLAYGVAVLHEPPVAPSQLLSEDAAAEEIRLKGAIEALQAQIDEML
EGQHGLVDASYEVLETYRMFAHDRGWNRSLEDAVRNGLTAEAAVERVRSEHRARLGQARDPYLRERLHDLEDLNDRLLRH
LAGDGHVARDLPENAILIARNLGPADLLEYDRTRLRGLLLEEGSSASHAAIVARALDIPCVGRLPGLRDRVSEGDPVIVD
AETGEAYLRPRTDVVKAIQARIDVRRQRQAAFAKLRETPAFTRDGVKITLLMNAGLDVDLESMADAGAEGIGLFRTEFQF
MVSEEMPRFNAQAALYGRVMDAAGDKPVTFRTLDLGGDKVLPYLEAEREDNPALGWRAIRMGLDRPALLRLQLRALIAAA
KGRALRIMFPLVASCDEFRAARALVDTEVAWALRRGRPAPSRLDVGAMIEAPSVIWHLDALLPMTDFVSVGTNDLMQYLF
AADRGNPRVADRYDPLSPPALRALETIQRACAETGTPVSVCGEMAGRPLEAFTLVALGFERLSMPPAGIGPVKQMVLSCD
REAARRGVSALLKSGAGSVRNEIETLARKLYLAV

Sequences:

>Translated_754_residues
MPAQGMAIRGQRSLLRQIREALAGGGPAQARLDMVVRIIALSMVAEVCSIYLRRATGEMELFATEGLEPGAVHVTRMKPG
EGLVGEIMRLGRPLNLADAPEHPAFSYRPETGEDPYHAFLGVPLLRGGRAIGVLVVQNRTARVYTEDEVEDLQIIAMVLA
EMVASGELIGVEEMNGVELAPRRPDRLKGSRFAEGLAYGVAVLHEPPVAPSQLLSEDAAAEEIRLKGAIEALQAQIDEML
EGQHGLVDASYEVLETYRMFAHDRGWNRSLEDAVRNGLTAEAAVERVRSEHRARLGQARDPYLRERLHDLEDLNDRLLRH
LAGDGHVARDLPENAILIARNLGPADLLEYDRTRLRGLLLEEGSSASHAAIVARALDIPCVGRLPGLRDRVSEGDPVIVD
AETGEAYLRPRTDVVKAIQARIDVRRQRQAAFAKLRETPAFTRDGVKITLLMNAGLDVDLESMADAGAEGIGLFRTEFQF
MVSEEMPRFNAQAALYGRVMDAAGDKPVTFRTLDLGGDKVLPYLEAEREDNPALGWRAIRMGLDRPALLRLQLRALIAAA
KGRALRIMFPLVASCDEFRAARALVDTEVAWALRRGRPAPSRLDVGAMIEAPSVIWHLDALLPMTDFVSVGTNDLMQYLF
AADRGNPRVADRYDPLSPPALRALETIQRACAETGTPVSVCGEMAGRPLEAFTLVALGFERLSMPPAGIGPVKQMVLSCD
REAARRGVSALLKSGAGSVRNEIETLARKLYLAV
>Mature_753_residues
PAQGMAIRGQRSLLRQIREALAGGGPAQARLDMVVRIIALSMVAEVCSIYLRRATGEMELFATEGLEPGAVHVTRMKPGE
GLVGEIMRLGRPLNLADAPEHPAFSYRPETGEDPYHAFLGVPLLRGGRAIGVLVVQNRTARVYTEDEVEDLQIIAMVLAE
MVASGELIGVEEMNGVELAPRRPDRLKGSRFAEGLAYGVAVLHEPPVAPSQLLSEDAAAEEIRLKGAIEALQAQIDEMLE
GQHGLVDASYEVLETYRMFAHDRGWNRSLEDAVRNGLTAEAAVERVRSEHRARLGQARDPYLRERLHDLEDLNDRLLRHL
AGDGHVARDLPENAILIARNLGPADLLEYDRTRLRGLLLEEGSSASHAAIVARALDIPCVGRLPGLRDRVSEGDPVIVDA
ETGEAYLRPRTDVVKAIQARIDVRRQRQAAFAKLRETPAFTRDGVKITLLMNAGLDVDLESMADAGAEGIGLFRTEFQFM
VSEEMPRFNAQAALYGRVMDAAGDKPVTFRTLDLGGDKVLPYLEAEREDNPALGWRAIRMGLDRPALLRLQLRALIAAAK
GRALRIMFPLVASCDEFRAARALVDTEVAWALRRGRPAPSRLDVGAMIEAPSVIWHLDALLPMTDFVSVGTNDLMQYLFA
ADRGNPRVADRYDPLSPPALRALETIQRACAETGTPVSVCGEMAGRPLEAFTLVALGFERLSMPPAGIGPVKQMVLSCDR
EAARRGVSALLKSGAGSVRNEIETLARKLYLAV

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=748, Percent_Identity=35.5614973262032, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788756, Length=449, Percent_Identity=34.5211581291759, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1788726, Length=549, Percent_Identity=33.1511839708561, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI48994992, Length=484, Percent_Identity=30.7851239669421, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI1787994, Length=486, Percent_Identity=24.2798353909465, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82411; Mature: 82280

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQGMAIRGQRSLLRQIREALAGGGPAQARLDMVVRIIALSMVAEVCSIYLRRATGEME
CCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LFATEGLEPGAVHVTRMKPGEGLVGEIMRLGRPLNLADAPEHPAFSYRPETGEDPYHAFL
EEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
GVPLLRGGRAIGVLVVQNRTARVYTEDEVEDLQIIAMVLAEMVASGELIGVEEMNGVELA
CCHHHCCCCEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
PRRPDRLKGSRFAEGLAYGVAVLHEPPVAPSQLLSEDAAAEEIRLKGAIEALQAQIDEML
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGQHGLVDASYEVLETYRMFAHDRGWNRSLEDAVRNGLTAEAAVERVRSEHRARLGQARD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
PYLRERLHDLEDLNDRLLRHLAGDGHVARDLPENAILIARNLGPADLLEYDRTRLRGLLL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
EEGSSASHAAIVARALDIPCVGRLPGLRDRVSEGDPVIVDAETGEAYLRPRTDVVKAIQA
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHH
RIDVRRQRQAAFAKLRETPAFTRDGVKITLLMNAGLDVDLESMADAGAEGIGLFRTEFQF
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHH
MVSEEMPRFNAQAALYGRVMDAAGDKPVTFRTLDLGGDKVLPYLEAEREDNPALGWRAIR
HHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCHHHHHHH
MGLDRPALLRLQLRALIAAAKGRALRIMFPLVASCDEFRAARALVDTEVAWALRRGRPAP
HCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCC
SRLDVGAMIEAPSVIWHLDALLPMTDFVSVGTNDLMQYLFAADRGNPRVADRYDPLSPPA
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
LRALETIQRACAETGTPVSVCGEMAGRPLEAFTLVALGFERLSMPPAGIGPVKQMVLSCD
HHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
REAARRGVSALLKSGAGSVRNEIETLARKLYLAV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAQGMAIRGQRSLLRQIREALAGGGPAQARLDMVVRIIALSMVAEVCSIYLRRATGEME
CCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LFATEGLEPGAVHVTRMKPGEGLVGEIMRLGRPLNLADAPEHPAFSYRPETGEDPYHAFL
EEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
GVPLLRGGRAIGVLVVQNRTARVYTEDEVEDLQIIAMVLAEMVASGELIGVEEMNGVELA
CCHHHCCCCEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
PRRPDRLKGSRFAEGLAYGVAVLHEPPVAPSQLLSEDAAAEEIRLKGAIEALQAQIDEML
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGQHGLVDASYEVLETYRMFAHDRGWNRSLEDAVRNGLTAEAAVERVRSEHRARLGQARD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
PYLRERLHDLEDLNDRLLRHLAGDGHVARDLPENAILIARNLGPADLLEYDRTRLRGLLL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
EEGSSASHAAIVARALDIPCVGRLPGLRDRVSEGDPVIVDAETGEAYLRPRTDVVKAIQA
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHH
RIDVRRQRQAAFAKLRETPAFTRDGVKITLLMNAGLDVDLESMADAGAEGIGLFRTEFQF
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHH
MVSEEMPRFNAQAALYGRVMDAAGDKPVTFRTLDLGGDKVLPYLEAEREDNPALGWRAIR
HHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCHHHHHHH
MGLDRPALLRLQLRALIAAAKGRALRIMFPLVASCDEFRAARALVDTEVAWALRRGRPAP
HCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCC
SRLDVGAMIEAPSVIWHLDALLPMTDFVSVGTNDLMQYLFAADRGNPRVADRYDPLSPPA
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
LRALETIQRACAETGTPVSVCGEMAGRPLEAFTLVALGFERLSMPPAGIGPVKQMVLSCD
HHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
REAARRGVSALLKSGAGSVRNEIETLARKLYLAV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]