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The map label for this gene is yngI [H]

Identifier: 197104199

GI number: 197104199

Start: 804902

End: 806437

Strand: Reverse

Name: yngI [H]

Synonym: PHZ_c0733

Alternate gene names: 197104199

Gene position: 806437-804902 (Counterclockwise)

Preceding gene: 197104202

Following gene: 197104196

Centisome position: 20.18

GC content: 70.44

Gene sequence:

>1536_bases
ATGGGCGAACTTACCAGCGGCGCACGGCGGCTGCCGATGGGCGATCTCCAGCAACGGGCGGCGCGCGCGGCCGCGGGCCT
GGCCAGCCTGGGCGTCGGCCGGGGCGATCTGGTCGCGCTCTACCTGCGCAACGATTTTCCGTTCTTCGAGGCGAGTTTCG
CCGCCGGCCTGATCGGCGCCTATCCGACGCCGGTGAACTGGCACTACACGGCCGACGAAGCGCGCTACCTGTTCGAGAAT
TCAGGCGCCAAGGCGATCGTCATCCACGCCGACCTCATCGAGGGGATCCGCGAGGCCCTGCCGCCCGGCGTGCCGGTGCT
GGCGGTCGCAACCCCGGCCGAGATCGCGGAGGCTTACGGGGTGGAAGCCGCGCCGGTCCCGCCCGGCATGACCGACTGGA
GCGCCTGGCTGGAGGGCTTCCCGCCGGCCGAGGCGACCACGACCGAGGCGCCGGGGACCGTCATCTACACCTCAGGCACC
ACCGGCCATCCCAAGGGCGTCCGCCGCGCGCCGCCGACGCCGGAAGAGGCGATGGTGACGATGACGATCGTCGGCCGCGC
GTTCGGCTTCATCGGCCACGGCGATCCGCAGGACATCGTCACCGTGATGACGGGCCCGATGTACCACTCGGCGCCGAACG
CCTATGGCCTGGCCGCCGCCCGCCTGGGCGCCAAGGTGATCCTGCAGCCGCGCTTCGACCCCGAGGAGCTGCTGCAGCTC
ATCGCCCGCGAGAAGGTCACGCACCTGCACATGGTGCCGATCATGTTCCACCGGCTGCTCAAGCTGCCCGACGAGGTGAA
GGCGAAGTACGACCTCTCCTCGCTCCGGTTCGTGGTCCACGCCGCCGCGCCCTGCCCGCCCCCGATCAAGCGGGCGATGA
TCGAGTGGTGGGGCCCGGTCATCAACGAGTACTACGGCTCGACCGAGACCAGCGCCGTGGTGTTCTGCACCTCCGAGGAC
TGGCTGGCGCACCCAGGCACGGTCGGCAAGGCCTGGCCCGAGGCGGACGTGCGGGTGATCGACGCCGAAGGCAATTCCCT
GCCTCCGGGCGAGGTCGGCGAGGTGGTGGCGCGCATCCGCGGGATGGCCGACTTCACCTACCACGGCGACGACGCCAAGC
GCCGGGCCAGCGAACGGGTGGGCCTGATCGCGCCAGGCGACATCGGCTACTTCGACAAGGACGGCTTCCTGTACCTCTGC
GACCGGGCCAAGGACATGATCATCTCGGGCGGGGTGAACATCTACCCGGCCGAGATCGAGGCCGAGCTGCTGAAGATGCC
CGGCGTCGCCGACTGCGCCGTCTTCGGCATCCCCGACGAGGAGTTCGGCGAGGCGGTCTGCGCGATCGTCCAGCCGCAGC
CGGGCGTGGAGATGGACGAGGCGGCGGTGAAGGCCTACCTGCGCGCCCACGTCGCCGGCTACAAGGTCCCCAAGCGCGTC
GAGCTCGCCGCCGAGCTGCCGCGCGAGGACTCGGGCAAGATCTTCAAGCGCAAGCTGCGCGAGCCCTACTGGGCCGGCCT
CGAGCGCCAGATCTAG

Upstream 100 bases:

>100_bases
TCCGCCGCAAACCGCCGGCTTCCGCGGGGGCATCTGGCGAGGGCGCGGAGGTTGGCGTTTACTGTCCCCGAGGCGCCGGC
AGAGCGCCCGGGAGGACGAC

Downstream 100 bases:

>100_bases
GCGCCGGATCTAGGCGCCGGATCTAGGCGACCGCCCGCAGCCGCGCCCTCGGGTCGGCGCCGGCCACGGTGAAGGAGCCC
ATCGTCCCGGCCAGATCGTC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MGELTSGARRLPMGDLQQRAARAAAGLASLGVGRGDLVALYLRNDFPFFEASFAAGLIGAYPTPVNWHYTADEARYLFEN
SGAKAIVIHADLIEGIREALPPGVPVLAVATPAEIAEAYGVEAAPVPPGMTDWSAWLEGFPPAEATTTEAPGTVIYTSGT
TGHPKGVRRAPPTPEEAMVTMTIVGRAFGFIGHGDPQDIVTVMTGPMYHSAPNAYGLAAARLGAKVILQPRFDPEELLQL
IAREKVTHLHMVPIMFHRLLKLPDEVKAKYDLSSLRFVVHAAAPCPPPIKRAMIEWWGPVINEYYGSTETSAVVFCTSED
WLAHPGTVGKAWPEADVRVIDAEGNSLPPGEVGEVVARIRGMADFTYHGDDAKRRASERVGLIAPGDIGYFDKDGFLYLC
DRAKDMIISGGVNIYPAEIEAELLKMPGVADCAVFGIPDEEFGEAVCAIVQPQPGVEMDEAAVKAYLRAHVAGYKVPKRV
ELAAELPREDSGKIFKRKLREPYWAGLERQI

Sequences:

>Translated_511_residues
MGELTSGARRLPMGDLQQRAARAAAGLASLGVGRGDLVALYLRNDFPFFEASFAAGLIGAYPTPVNWHYTADEARYLFEN
SGAKAIVIHADLIEGIREALPPGVPVLAVATPAEIAEAYGVEAAPVPPGMTDWSAWLEGFPPAEATTTEAPGTVIYTSGT
TGHPKGVRRAPPTPEEAMVTMTIVGRAFGFIGHGDPQDIVTVMTGPMYHSAPNAYGLAAARLGAKVILQPRFDPEELLQL
IAREKVTHLHMVPIMFHRLLKLPDEVKAKYDLSSLRFVVHAAAPCPPPIKRAMIEWWGPVINEYYGSTETSAVVFCTSED
WLAHPGTVGKAWPEADVRVIDAEGNSLPPGEVGEVVARIRGMADFTYHGDDAKRRASERVGLIAPGDIGYFDKDGFLYLC
DRAKDMIISGGVNIYPAEIEAELLKMPGVADCAVFGIPDEEFGEAVCAIVQPQPGVEMDEAAVKAYLRAHVAGYKVPKRV
ELAAELPREDSGKIFKRKLREPYWAGLERQI
>Mature_510_residues
GELTSGARRLPMGDLQQRAARAAAGLASLGVGRGDLVALYLRNDFPFFEASFAAGLIGAYPTPVNWHYTADEARYLFENS
GAKAIVIHADLIEGIREALPPGVPVLAVATPAEIAEAYGVEAAPVPPGMTDWSAWLEGFPPAEATTTEAPGTVIYTSGTT
GHPKGVRRAPPTPEEAMVTMTIVGRAFGFIGHGDPQDIVTVMTGPMYHSAPNAYGLAAARLGAKVILQPRFDPEELLQLI
AREKVTHLHMVPIMFHRLLKLPDEVKAKYDLSSLRFVVHAAAPCPPPIKRAMIEWWGPVINEYYGSTETSAVVFCTSEDW
LAHPGTVGKAWPEADVRVIDAEGNSLPPGEVGEVVARIRGMADFTYHGDDAKRRASERVGLIAPGDIGYFDKDGFLYLCD
RAKDMIISGGVNIYPAEIEAELLKMPGVADCAVFGIPDEEFGEAVCAIVQPQPGVEMDEAAVKAYLRAHVAGYKVPKRVE
LAAELPREDSGKIFKRKLREPYWAGLERQI

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=28.952380952381, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI42544132, Length=510, Percent_Identity=27.2549019607843, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI38505220, Length=514, Percent_Identity=28.7937743190661, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI122937307, Length=515, Percent_Identity=28.1553398058252, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=26.7716535433071, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI157311624, Length=518, Percent_Identity=27.4131274131274, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI157311622, Length=518, Percent_Identity=27.4131274131274, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI58082049, Length=521, Percent_Identity=27.2552783109405, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI187761345, Length=530, Percent_Identity=25.2830188679245, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI187761343, Length=530, Percent_Identity=25.2830188679245, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI195947378, Length=411, Percent_Identity=26.0340632603406, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI13325057, Length=547, Percent_Identity=24.8628884826325, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI13375727, Length=540, Percent_Identity=22.5925925925926, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1788107, Length=513, Percent_Identity=29.4346978557505, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=25.6756756756757, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI145693145, Length=509, Percent_Identity=27.1119842829077, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=24.3494423791822, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786810, Length=515, Percent_Identity=26.6019417475728, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=512, Percent_Identity=27.5390625, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71994694, Length=512, Percent_Identity=27.5390625, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=512, Percent_Identity=27.5390625, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17560308, Length=511, Percent_Identity=29.3542074363992, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=29.0513833992095, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=507, Percent_Identity=25.8382642998028, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=505, Percent_Identity=27.1287128712871, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=509, Percent_Identity=26.5225933202358, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=25.8823529411765, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17551278, Length=575, Percent_Identity=23.4782608695652, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=351, Percent_Identity=26.4957264957265, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=25.5376344086022, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=25.6214149139579, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=530, Percent_Identity=23.3962264150943, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25141359, Length=371, Percent_Identity=25.3369272237197, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=528, Percent_Identity=23.2954545454545, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=528, Percent_Identity=23.2954545454545, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=510, Percent_Identity=25.0980392156863, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=519, Percent_Identity=25.626204238921, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355181, Length=499, Percent_Identity=29.0581162324649, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=27.2556390977444, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922652, Length=514, Percent_Identity=27.0428015564202, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24656500, Length=515, Percent_Identity=25.0485436893204, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648253, Length=509, Percent_Identity=24.75442043222, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=27.2727272727273, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648255, Length=492, Percent_Identity=25.2032520325203, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648260, Length=351, Percent_Identity=27.3504273504274, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281365686, Length=466, Percent_Identity=23.8197424892704, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358303, Length=471, Percent_Identity=23.1422505307856, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21356441, Length=475, Percent_Identity=24, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356947, Length=492, Percent_Identity=22.9674796747967, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653035, Length=357, Percent_Identity=25.2100840336134, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648257, Length=358, Percent_Identity=25.4189944134078, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573631, Length=550, Percent_Identity=24, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI20130353, Length=550, Percent_Identity=24, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161076582, Length=364, Percent_Identity=25, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI20130357, Length=372, Percent_Identity=24.4623655913978, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21355225, Length=541, Percent_Identity=24.5841035120148, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648676, Length=387, Percent_Identity=24.5478036175711, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 55265; Mature: 55134

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELTSGARRLPMGDLQQRAARAAAGLASLGVGRGDLVALYLRNDFPFFEASFAAGLIGA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCC
YPTPVNWHYTADEARYLFENSGAKAIVIHADLIEGIREALPPGVPVLAVATPAEIAEAYG
CCCCCCEEEEHHHHHHHEECCCCCEEEEEHHHHHHHHHHCCCCCCEEEEECHHHHHHHHC
VEAAPVPPGMTDWSAWLEGFPPAEATTTEAPGTVIYTSGTTGHPKGVRRAPPTPEEAMVT
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHEEE
MTIVGRAFGFIGHGDPQDIVTVMTGPMYHSAPNAYGLAAARLGAKVILQPRFDPEELLQL
EEEHHHHHHCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHH
IAREKVTHLHMVPIMFHRLLKLPDEVKAKYDLSSLRFVVHAAAPCPPPIKRAMIEWWGPV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHHHHHH
INEYYGSTETSAVVFCTSEDWLAHPGTVGKAWPEADVRVIDAEGNSLPPGEVGEVVARIR
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GMADFTYHGDDAKRRASERVGLIAPGDIGYFDKDGFLYLCDRAKDMIISGGVNIYPAEIE
CCCCEEECCCHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCCEEEECCCEEEEHHHH
AELLKMPGVADCAVFGIPDEEFGEAVCAIVQPQPGVEMDEAAVKAYLRAHVAGYKVPKRV
HHHHHCCCCCCEEEECCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
ELAAELPREDSGKIFKRKLREPYWAGLERQI
HHHHHCCCCCCHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
GELTSGARRLPMGDLQQRAARAAAGLASLGVGRGDLVALYLRNDFPFFEASFAAGLIGA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCC
YPTPVNWHYTADEARYLFENSGAKAIVIHADLIEGIREALPPGVPVLAVATPAEIAEAYG
CCCCCCEEEEHHHHHHHEECCCCCEEEEEHHHHHHHHHHCCCCCCEEEEECHHHHHHHHC
VEAAPVPPGMTDWSAWLEGFPPAEATTTEAPGTVIYTSGTTGHPKGVRRAPPTPEEAMVT
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHEEE
MTIVGRAFGFIGHGDPQDIVTVMTGPMYHSAPNAYGLAAARLGAKVILQPRFDPEELLQL
EEEHHHHHHCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHH
IAREKVTHLHMVPIMFHRLLKLPDEVKAKYDLSSLRFVVHAAAPCPPPIKRAMIEWWGPV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHHHHHH
INEYYGSTETSAVVFCTSEDWLAHPGTVGKAWPEADVRVIDAEGNSLPPGEVGEVVARIR
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GMADFTYHGDDAKRRASERVGLIAPGDIGYFDKDGFLYLCDRAKDMIISGGVNIYPAEIE
CCCCEEECCCHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCCEEEECCCEEEEHHHH
AELLKMPGVADCAVFGIPDEEFGEAVCAIVQPQPGVEMDEAAVKAYLRAHVAGYKVPKRV
HHHHHCCCCCCEEEECCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
ELAAELPREDSGKIFKRKLREPYWAGLERQI
HHHHHCCCCCCHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]