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The map label for this gene is pmbA [H]

Identifier: 197103985

GI number: 197103985

Start: 544478

End: 545818

Strand: Reverse

Name: pmbA [H]

Synonym: PHZ_c0519

Alternate gene names: 197103985

Gene position: 545818-544478 (Counterclockwise)

Preceding gene: 197103986

Following gene: 197103984

Centisome position: 13.66

GC content: 74.65

Gene sequence:

>1341_bases
ATGCCGGGCATGGACGAAAACCTGCTGAACGACGTCGTGGCCGCCGCGCTGAAGGCCGGGGCGGACGCCGCCGAGGCCGT
GGGCGCCGAGCGCCGCAGCCTTTCGGTCACCGTGCGCCTGGGCGACCTCGAGGAGGTGGAGCGCGAGGAGTCCCGCGACC
TCGGCCTGCGGGTGTTCGTCGGCCGCCGGCAGGCGACCGTCTCCGGCTCCGACATTTCGCCGGAGGCGCGCGCCAAGCTG
GTCGAGCGGGCGGTCGCCATGGCCAAGCTCGCCCCGGAGGATCCCTACGCAGGCCTGGCCGACCCCGACGCCCTGGCCCG
CGGCGACCTCCCCGACCTCGACCTCTACGACCCGGCCGAGCCCACCGCGGCCGAGCTGGAGGATCGCGCCCGCGCCGCCG
AGGCCGCCGCCCGCGCCCAGCCCAGGGTCACCAACACCGACGGCGCCAGCGGCGCGTGGTCGTCCTCGCGCTGGACCATC
GTCACCAGCGGCGGCTTCACGGGCGTGCACCGCGCTTCGGGCTTCTCGATCGGCGCCTCGGCCATCGCCGGCGACGAGGG
CGGCATGGAGACCGGCTACGACGGCCGCTCGGCCCGCTGGCAGGCCGACCTGCCCGCGCCCGAGACCCTGGGCGCCGAGG
CCGGCCGCCGCGCCGCCGCCCGCCTGGGCGCGCGCAAGATCGCCTCGACCACCGCGCCGGTGATCTTCGAGAACCGCCTG
GCCGCCTCGCTGCTGTCGCCGTTGATCGGCGCGATCGCCGGCCCCTCCATCGCCCGCGGGACCTCGTTCCTGAAGGACAG
GCTGGGCCAGCCGGTGTTCGCCAAGGGGATCTTCGTCACCGACGATCCGCACCGTCCGCGCGGCCTCGGCTCCTCGCCGT
TCGACGACGAGGGGGTCCGCAACCAGCGCCGCGACCTGATCGAGGACGGGGTGCTCACCACCTGGCTGCTGAACTCGTCC
TCGGCCCGCCAGCTGGGCCTTTCGACGACCGGCCACGCCTCGCGCGGCCTGGCCGGCCCGCCCGGCGTCTCGCCCACCAA
CCTGACCCTGCGGCCGGGCGAGAAGGACCTCTCCGCCCTGATGAAGGACGCCGGCTCGGGCCTGCTCGTCACCTCGATGT
TCGGCCCTTCGCTGAACGGCAACACCGGCGACTGGTCGGTGGGCTGCTCGGGCTTCTGGTTCGAGGGCGGCGAGCTCGCC
TATCCGGTCAGCGAGATCACCGTCGCCGGCAACCTGATCGACATCTACGGCCGCCTCGTCCCGGGCTCGGACCTCGAAAT
CCGCGGCGCCTCCAACGCGCCCTCCCTGCTGGTGGACGCCCTGGCCATCGCGGGGCGCTGA

Upstream 100 bases:

>100_bases
GCGGGCGTCCTCGCCACCTGGATCGCCTATATCGCCGCGGCCGCCGCGCTGCTGTTCTACGCGGTGCCGTGAGCTTGTGA
ACGGGGCTCCGCTCGGCCAT

Downstream 100 bases:

>100_bases
TCCTGGACGACCTGGCCCTCCTCCTGGACGCCGCGCAGGAAGCGGGCGAGCTCGCGGCCCGCCTGCGCCGCGAGGGCCTG
GAGATCGACTACAAGGAGGG

Product: predicted Zn-dependent protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPGMDENLLNDVVAAALKAGADAAEAVGAERRSLSVTVRLGDLEEVEREESRDLGLRVFVGRRQATVSGSDISPEARAKL
VERAVAMAKLAPEDPYAGLADPDALARGDLPDLDLYDPAEPTAAELEDRARAAEAAARAQPRVTNTDGASGAWSSSRWTI
VTSGGFTGVHRASGFSIGASAIAGDEGGMETGYDGRSARWQADLPAPETLGAEAGRRAAARLGARKIASTTAPVIFENRL
AASLLSPLIGAIAGPSIARGTSFLKDRLGQPVFAKGIFVTDDPHRPRGLGSSPFDDEGVRNQRRDLIEDGVLTTWLLNSS
SARQLGLSTTGHASRGLAGPPGVSPTNLTLRPGEKDLSALMKDAGSGLLVTSMFGPSLNGNTGDWSVGCSGFWFEGGELA
YPVSEITVAGNLIDIYGRLVPGSDLEIRGASNAPSLLVDALAIAGR

Sequences:

>Translated_446_residues
MPGMDENLLNDVVAAALKAGADAAEAVGAERRSLSVTVRLGDLEEVEREESRDLGLRVFVGRRQATVSGSDISPEARAKL
VERAVAMAKLAPEDPYAGLADPDALARGDLPDLDLYDPAEPTAAELEDRARAAEAAARAQPRVTNTDGASGAWSSSRWTI
VTSGGFTGVHRASGFSIGASAIAGDEGGMETGYDGRSARWQADLPAPETLGAEAGRRAAARLGARKIASTTAPVIFENRL
AASLLSPLIGAIAGPSIARGTSFLKDRLGQPVFAKGIFVTDDPHRPRGLGSSPFDDEGVRNQRRDLIEDGVLTTWLLNSS
SARQLGLSTTGHASRGLAGPPGVSPTNLTLRPGEKDLSALMKDAGSGLLVTSMFGPSLNGNTGDWSVGCSGFWFEGGELA
YPVSEITVAGNLIDIYGRLVPGSDLEIRGASNAPSLLVDALAIAGR
>Mature_445_residues
PGMDENLLNDVVAAALKAGADAAEAVGAERRSLSVTVRLGDLEEVEREESRDLGLRVFVGRRQATVSGSDISPEARAKLV
ERAVAMAKLAPEDPYAGLADPDALARGDLPDLDLYDPAEPTAAELEDRARAAEAAARAQPRVTNTDGASGAWSSSRWTIV
TSGGFTGVHRASGFSIGASAIAGDEGGMETGYDGRSARWQADLPAPETLGAEAGRRAAARLGARKIASTTAPVIFENRLA
ASLLSPLIGAIAGPSIARGTSFLKDRLGQPVFAKGIFVTDDPHRPRGLGSSPFDDEGVRNQRRDLIEDGVLTTWLLNSSS
ARQLGLSTTGHASRGLAGPPGVSPTNLTLRPGEKDLSALMKDAGSGLLVTSMFGPSLNGNTGDWSVGCSGFWFEGGELAY
PVSEITVAGNLIDIYGRLVPGSDLEIRGASNAPSLLVDALAIAGR

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=448, Percent_Identity=39.7321428571429, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789640, Length=251, Percent_Identity=27.0916334661355, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46264; Mature: 46133

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGMDENLLNDVVAAALKAGADAAEAVGAERRSLSVTVRLGDLEEVEREESRDLGLRVFV
CCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCEEEEE
GRRQATVSGSDISPEARAKLVERAVAMAKLAPEDPYAGLADPDALARGDLPDLDLYDPAE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
PTAAELEDRARAAEAAARAQPRVTNTDGASGAWSSSRWTIVTSGGFTGVHRASGFSIGAS
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCH
AIAGDEGGMETGYDGRSARWQADLPAPETLGAEAGRRAAARLGARKIASTTAPVIFENRL
HCCCCCCCCCCCCCCCCCEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AASLLSPLIGAIAGPSIARGTSFLKDRLGQPVFAKGIFVTDDPHRPRGLGSSPFDDEGVR
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCCCCCCCHHH
NQRRDLIEDGVLTTWLLNSSSARQLGLSTTGHASRGLAGPPGVSPTNLTLRPGEKDLSAL
HHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
MKDAGSGLLVTSMFGPSLNGNTGDWSVGCSGFWFEGGELAYPVSEITVAGNLIDIYGRLV
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECCHHHHEECCCHHHHHHCCC
PGSDLEIRGASNAPSLLVDALAIAGR
CCCCEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PGMDENLLNDVVAAALKAGADAAEAVGAERRSLSVTVRLGDLEEVEREESRDLGLRVFV
CCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCEEEEE
GRRQATVSGSDISPEARAKLVERAVAMAKLAPEDPYAGLADPDALARGDLPDLDLYDPAE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
PTAAELEDRARAAEAAARAQPRVTNTDGASGAWSSSRWTIVTSGGFTGVHRASGFSIGAS
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCH
AIAGDEGGMETGYDGRSARWQADLPAPETLGAEAGRRAAARLGARKIASTTAPVIFENRL
HCCCCCCCCCCCCCCCCCEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AASLLSPLIGAIAGPSIARGTSFLKDRLGQPVFAKGIFVTDDPHRPRGLGSSPFDDEGVR
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCCCCCCCHHH
NQRRDLIEDGVLTTWLLNSSSARQLGLSTTGHASRGLAGPPGVSPTNLTLRPGEKDLSAL
HHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
MKDAGSGLLVTSMFGPSLNGNTGDWSVGCSGFWFEGGELAYPVSEITVAGNLIDIYGRLV
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECCHHHHEECCCHHHHHHCCC
PGSDLEIRGASNAPSLLVDALAIAGR
CCCCEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]