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The map label for this gene is rpoH [H]

Identifier: 197103875

GI number: 197103875

Start: 414395

End: 415282

Strand: Reverse

Name: rpoH [H]

Synonym: PHZ_c0409

Alternate gene names: 197103875

Gene position: 415282-414395 (Counterclockwise)

Preceding gene: 197103876

Following gene: 197103872

Centisome position: 10.39

GC content: 67.12

Gene sequence:

>888_bases
ATGGCCGCCAATGCGCTCGCCGTGATGTCGCCGGAAGGCGGCCTCAGCCGGTATCTGACTGAAATCCGCAAGTTCCCGAT
GCTCGCGAAGGACGAGGAGTTCATGCTGGCCAAGCGCTGGCAGGAGCACCAGGATCCCGAGGCGGCGCACCGGCTCGTCA
CCAGCCACCTGCGGCTCGTGGCCAAGATCGCCATGGGCTATCGCGGCTACGGCCTGCCGATCGGCGAGGTGATCTCCGAG
GGCAACGTCGGCCTGATGCAGGCGGTCAAGAAGTTCGACCCCGACAAGGGCTTCCGCCTGGCCACCTACGCCATGTGGTG
GATCCGCGCCTCGATCCAGGAATACATCCTCCGCTCCTGGTCGCTGGTCAAAATGGGCACGACCGCCGCCCAGAAGAAGC
TTTTCTTCAATTTGAGAAAGGCCAAGAGCGAAATCTCGGCTCTGGAGGAGGGCGACCTGCGTCCCGAGCAGGTCCGCCAG
ATCGCCACCAAGCTCGGCGTGCTGGACGAGGAAGTCATCTCCATGAACCGGCGCCTCGCCGGCGGGGACGCCTCGCTGAA
CTCGCCGCTGCGGGCCGATTCCGAGAGCGAGTGGCAGGACTGGCTGGTCGACGAGGACACGCCCTCGCAGGAGAGCGTCG
TCGCCGAGGCCGAGGAGCGCGGCATCCGCATGGAGCTGCTGCAGGAGGCCATGGCCGAGCTCACCGACCGCGAGCGCCAC
ATCCTGACCGAGCGGCGGCTGAAGGACGAGCCCACGACCCTCGAGGAGCTCGCCTCGCAGTACGGCGTCAGCCGCGAGCG
GGTGCGCCAGATCGAGGTCCGCGCCTTCGAGAAGCTGCAGAAGTCGATGCGCGCGGCGGCCGAGGAGCGGAACCTGGTCG
AGGCCTAG

Upstream 100 bases:

>100_bases
CCTGCAACCTGGCGCCTATCTGAAAGGTTGGGGGGCGACGGTCCTTGCGCGCGGGTGTCGCGCGCCGGCCTGAAGTCCGG
TCCGAGAGGGGATAGTGTCC

Downstream 100 bases:

>100_bases
GCCGCGACCCCCTCGTCCTTGGGCATGTAGGCCATCAGCGAGGCGGCGTGCCTGGCCACGTCGGTCGCGCTGATGGCGAC
GCCGGTCTTGCGCGACAGCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAANALAVMSPEGGLSRYLTEIRKFPMLAKDEEFMLAKRWQEHQDPEAAHRLVTSHLRLVAKIAMGYRGYGLPIGEVISE
GNVGLMQAVKKFDPDKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSEISALEEGDLRPEQVRQ
IATKLGVLDEEVISMNRRLAGGDASLNSPLRADSESEWQDWLVDEDTPSQESVVAEAEERGIRMELLQEAMAELTDRERH
ILTERRLKDEPTTLEELASQYGVSRERVRQIEVRAFEKLQKSMRAAAEERNLVEA

Sequences:

>Translated_295_residues
MAANALAVMSPEGGLSRYLTEIRKFPMLAKDEEFMLAKRWQEHQDPEAAHRLVTSHLRLVAKIAMGYRGYGLPIGEVISE
GNVGLMQAVKKFDPDKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSEISALEEGDLRPEQVRQ
IATKLGVLDEEVISMNRRLAGGDASLNSPLRADSESEWQDWLVDEDTPSQESVVAEAEERGIRMELLQEAMAELTDRERH
ILTERRLKDEPTTLEELASQYGVSRERVRQIEVRAFEKLQKSMRAAAEERNLVEA
>Mature_294_residues
AANALAVMSPEGGLSRYLTEIRKFPMLAKDEEFMLAKRWQEHQDPEAAHRLVTSHLRLVAKIAMGYRGYGLPIGEVISEG
NVGLMQAVKKFDPDKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSEISALEEGDLRPEQVRQI
ATKLGVLDEEVISMNRRLAGGDASLNSPLRADSESEWQDWLVDEDTPSQESVVAEAEERGIRMELLQEAMAELTDRERHI
LTERRLKDEPTTLEELASQYGVSRERVRQIEVRAFEKLQKSMRAAAEERNLVEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=292, Percent_Identity=39.3835616438356, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=34.5588235294118, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=31.0483870967742, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33636; Mature: 33505

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANALAVMSPEGGLSRYLTEIRKFPMLAKDEEFMLAKRWQEHQDPEAAHRLVTSHLRLV
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFDPDKGFRLATYAMWWIRASIQEYILRSW
HHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLVKMGTTAAQKKLFFNLRKAKSEISALEEGDLRPEQVRQIATKLGVLDEEVISMNRRLA
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
GGDASLNSPLRADSESEWQDWLVDEDTPSQESVVAEAEERGIRMELLQEAMAELTDRERH
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ILTERRLKDEPTTLEELASQYGVSRERVRQIEVRAFEKLQKSMRAAAEERNLVEA
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AANALAVMSPEGGLSRYLTEIRKFPMLAKDEEFMLAKRWQEHQDPEAAHRLVTSHLRLV
CCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFDPDKGFRLATYAMWWIRASIQEYILRSW
HHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLVKMGTTAAQKKLFFNLRKAKSEISALEEGDLRPEQVRQIATKLGVLDEEVISMNRRLA
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
GGDASLNSPLRADSESEWQDWLVDEDTPSQESVVAEAEERGIRMELLQEAMAELTDRERH
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ILTERRLKDEPTTLEELASQYGVSRERVRQIEVRAFEKLQKSMRAAAEERNLVEA
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]