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The map label for this gene is purA

Identifier: 197103873

GI number: 197103873

Start: 412219

End: 413505

Strand: Direct

Name: purA

Synonym: PHZ_c0407

Alternate gene names: 197103873

Gene position: 412219-413505 (Clockwise)

Preceding gene: 197103870

Following gene: 197103874

Centisome position: 10.32

GC content: 71.41

Gene sequence:

>1287_bases
ATGGCCAACGTGACGGTGATCGGCGCCCAGTGGGGCGACGAGGGCAAGGGCAAGCTGGTGGACTGGCTGTCCAACCGCGC
CGACGTGGTGGTGCGTTTCCAGGGCGGCCACAACGCGGGCCACACCCTGGTGGTGGGGAACCAGACCTACAAGCTCTCGC
TGCTGCCGTCGGGCGTGGTGCAGGGCAAGCTGTCGATCATCGGCAACGGCGTGGTGGTCGATCCCTGGCACCTGCTGAAG
GAGATCGAGACCCTGGGCGCGCAGGGCGTGTCGATCGGGCCGGATCTGCTGGTGCTGGCGGACAACGCCTGCCTGATCCT
GCCCCTGCACCGCGACCTCGACCAGGCGCGCGAGGCGGCCGCGACGAACAAGATCGGTACGACGGGCCGCGGCATCGGGC
CCGCCTACGAGGACAAGGTCGGCCGCCGCGCCATCCGGGTCGCCGACCTGCACGATCGCGAGGCGCTGGAGGCCAAGATC
GACCGCCTGCTCGCCCACCACACCCCGCTGCGCCGCGGTCTCGGGCTGCCCGAGTACGACGGCCCGGCGCTGCTGGCCGA
GCTGGAGGCCCTTGCGCCGAAGATCCTGCCCTACGCGCGGCCCGCCTGGCTGATGCTGGACGAGATCGTCAAGGCCGGCA
GCCGCGTGCTGTTCGAGGGCGCGCAAGGGGCGCTGCTGGACGTGGATCACGGCACCTATCCGTACGTGACCTCCTCGAAC
ACCGTGGCCGGCCAGGCCGCGGCCGGCTCGGGCCTGGGGCCGAAGGGACCGGGCTACGTCCTGGGGATCGTCAAGGCCTA
CACCACCCGCGTGGGCGAGGGACCGTTCCCGACCGAGCTGAACGACGAGGTCGGCCAGCACCTGGGCACGGTGGGCCGCG
AGTTCGGCACCGTCACCGGCCGCTCGCGCCGGTGCGGCTGGTTCGACGCCGCCCTGGTGCGCCAGTCGGTGGCCCTGAAC
GGCATCGCCGGAATTGCGCTGACCAAGCTCGATGTCCTCGACGGCCTGCCGACGCTGAAGATCTGCACGGGCTACCGGCT
CGGGGACCGCGAGATCGGCTACCTGCCCGCCGGCCTGAAGGCGCAGCGCGAGCTCGCGCCGGTCTACGAGGAGATGGAGG
GCTGGTCCGAGACCACCCAGGGCGCCCGCTCGTGGAAGGACCTGCCGGCCAACGCCGTGAAGTACGTCCGGCGCATCGAG
GAGCTGATCGGCGCGCCGGTGGCCCTGCTCTCCACCAGCCCGCAGCGCGACGACACGATCCTGATGCGCGACCCCTTCCA
GGACTGA

Upstream 100 bases:

>100_bases
TTGGTCTAAACGGGCGCCCGCAATCGATCTCCGCCTCCCCGCCGAGCACCTGTCGAAGGGCCGGGAGGCGTCTTCCGGGC
AGGTGGGGAGATCGTATCTC

Downstream 100 bases:

>100_bases
CGCTGCGACGATCGGCGTCACGCCGCGTCAAGCGGTTCTTTACCGGAACGCGTTATGCGGGCCCCCAGGGAGCGGAGCCC
GCCGTGTTCGACGACGAGAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MANVTVIGAQWGDEGKGKLVDWLSNRADVVVRFQGGHNAGHTLVVGNQTYKLSLLPSGVVQGKLSIIGNGVVVDPWHLLK
EIETLGAQGVSIGPDLLVLADNACLILPLHRDLDQAREAAATNKIGTTGRGIGPAYEDKVGRRAIRVADLHDREALEAKI
DRLLAHHTPLRRGLGLPEYDGPALLAELEALAPKILPYARPAWLMLDEIVKAGSRVLFEGAQGALLDVDHGTYPYVTSSN
TVAGQAAAGSGLGPKGPGYVLGIVKAYTTRVGEGPFPTELNDEVGQHLGTVGREFGTVTGRSRRCGWFDAALVRQSVALN
GIAGIALTKLDVLDGLPTLKICTGYRLGDREIGYLPAGLKAQRELAPVYEEMEGWSETTQGARSWKDLPANAVKYVRRIE
ELIGAPVALLSTSPQRDDTILMRDPFQD

Sequences:

>Translated_428_residues
MANVTVIGAQWGDEGKGKLVDWLSNRADVVVRFQGGHNAGHTLVVGNQTYKLSLLPSGVVQGKLSIIGNGVVVDPWHLLK
EIETLGAQGVSIGPDLLVLADNACLILPLHRDLDQAREAAATNKIGTTGRGIGPAYEDKVGRRAIRVADLHDREALEAKI
DRLLAHHTPLRRGLGLPEYDGPALLAELEALAPKILPYARPAWLMLDEIVKAGSRVLFEGAQGALLDVDHGTYPYVTSSN
TVAGQAAAGSGLGPKGPGYVLGIVKAYTTRVGEGPFPTELNDEVGQHLGTVGREFGTVTGRSRRCGWFDAALVRQSVALN
GIAGIALTKLDVLDGLPTLKICTGYRLGDREIGYLPAGLKAQRELAPVYEEMEGWSETTQGARSWKDLPANAVKYVRRIE
ELIGAPVALLSTSPQRDDTILMRDPFQD
>Mature_427_residues
ANVTVIGAQWGDEGKGKLVDWLSNRADVVVRFQGGHNAGHTLVVGNQTYKLSLLPSGVVQGKLSIIGNGVVVDPWHLLKE
IETLGAQGVSIGPDLLVLADNACLILPLHRDLDQAREAAATNKIGTTGRGIGPAYEDKVGRRAIRVADLHDREALEAKID
RLLAHHTPLRRGLGLPEYDGPALLAELEALAPKILPYARPAWLMLDEIVKAGSRVLFEGAQGALLDVDHGTYPYVTSSNT
VAGQAAAGSGLGPKGPGYVLGIVKAYTTRVGEGPFPTELNDEVGQHLGTVGREFGTVTGRSRRCGWFDAALVRQSVALNG
IAGIALTKLDVLDGLPTLKICTGYRLGDREIGYLPAGLKAQRELAPVYEEMEGWSETTQGARSWKDLPANAVKYVRRIEE
LIGAPVALLSTSPQRDDTILMRDPFQD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=41.2735849056604, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=40.2810304449649, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=39.3939393939394, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=51.4018691588785, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=43.1603773584906, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=43.1603773584906, Blast_Score=331, Evalue=6e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=40.5092592592593, Blast_Score=318, Evalue=8e-88,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=41.7661097852029, Blast_Score=313, Evalue=1e-85,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PHEZH (B4RDX2)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129250.1
- GeneID:   6789897
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0407
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 45828; Mature: 45697

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 222-222 BINDING 237-237 BINDING 301-301 BINDING 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVTVIGAQWGDEGKGKLVDWLSNRADVVVRFQGGHNAGHTLVVGNQTYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEEECCCCHH
QGKLSIIGNGVVVDPWHLLKEIETLGAQGVSIGPDLLVLADNACLILPLHRDLDQAREAA
CCEEEEEECCEEECHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEECHHCHHHHHHHH
ATNKIGTTGRGIGPAYEDKVGRRAIRVADLHDREALEAKIDRLLAHHTPLRRGLGLPEYD
HHCCCCCCCCCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCC
GPALLAELEALAPKILPYARPAWLMLDEIVKAGSRVLFEGAQGALLDVDHGTYPYVTSSN
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECCC
TVAGQAAAGSGLGPKGPGYVLGIVKAYTTRVGEGPFPTELNDEVGQHLGTVGREFGTVTG
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RSRRCGWFDAALVRQSVALNGIAGIALTKLDVLDGLPTLKICTGYRLGDREIGYLPAGLK
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCC
AQRELAPVYEEMEGWSETTQGARSWKDLPANAVKYVRRIEELIGAPVALLSTSPQRDDTI
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
LMRDPFQD
EECCCCCC
>Mature Secondary Structure 
ANVTVIGAQWGDEGKGKLVDWLSNRADVVVRFQGGHNAGHTLVVGNQTYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEEECCCCHH
QGKLSIIGNGVVVDPWHLLKEIETLGAQGVSIGPDLLVLADNACLILPLHRDLDQAREAA
CCEEEEEECCEEECHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEECHHCHHHHHHHH
ATNKIGTTGRGIGPAYEDKVGRRAIRVADLHDREALEAKIDRLLAHHTPLRRGLGLPEYD
HHCCCCCCCCCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCC
GPALLAELEALAPKILPYARPAWLMLDEIVKAGSRVLFEGAQGALLDVDHGTYPYVTSSN
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECCC
TVAGQAAAGSGLGPKGPGYVLGIVKAYTTRVGEGPFPTELNDEVGQHLGTVGREFGTVTG
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RSRRCGWFDAALVRQSVALNGIAGIALTKLDVLDGLPTLKICTGYRLGDREIGYLPAGLK
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCC
AQRELAPVYEEMEGWSETTQGARSWKDLPANAVKYVRRIEELIGAPVALLSTSPQRDDTI
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
LMRDPFQD
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA