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The map label for this gene is truA [H]

Identifier: 197103844

GI number: 197103844

Start: 382581

End: 383321

Strand: Direct

Name: truA [H]

Synonym: PHZ_c0378

Alternate gene names: 197103844

Gene position: 382581-383321 (Clockwise)

Preceding gene: 197103843

Following gene: 197103846

Centisome position: 9.57

GC content: 73.14

Gene sequence:

>741_bases
CTGATGCCCCGCTACCGCCTCCTGATCGAATACGACGGCCGGCCGTTCAACGGCTTCCAGGCGCAGGACGGGCTGCCCAC
GATCCAGGGCGCCATCGAGCGGGCGGTGAAGGCGTTCTGCGGCGAGGACCTGCGCCTGCAGGCGGCGGGGCGGACCGACA
CCGGGGTGCACGCCCGGGGCCAGGTGGTGCACGTGGACCTCGCCAAGGACTGGCCGGCCGAGACCGTCCGCAACGCGCTG
AACGCCCACCTCGTCCCCCAGCCGATCGCGGTGCTGGAGGCGAGCGTCGCGCCCGAGGGGTGGCACGCCCGCTTCTCGGC
CAAGGAGCGGCGCTATCTCTACCGCATCCTCAACCGCATGGCGCCGCCGGCCCTGGACCAGGGGCGCGTGTGGCACGTGA
AGAAGCCGTTGGACGCCGAGGCCATGCACGAGGCCGCCCAGGCGCTGGTGGGCCACCACGACTTCACCACCTTCCGCGAC
CTCGGCTGCCAGGCGAAGTCGCCGATGAAGACGCTGGACCTGGCCAGCGTGCGCCGCGAGGGCGACGAGGTGCTGCTGGA
GTTCGCCTCGCGCTCGTTCCTGCACCGGCAGGTGCGCTCGATGACCGGAACCCTGGCCGAGGTCGGCGTCGGCCGCTGGA
CCAGGGCCGACCTCGTCGCGGCCCTGGAGGCCCGCGACCGCCGCGCCTGCGGGCCCGTGGCGCCGGCCGAGGGGCTCTAT
TTGACAGGGGTGGCCTACTGA

Upstream 100 bases:

>100_bases
GCGCGGTGCGCCTGCTGCGCGTGCAGCGCGAGGGGCGCGGGCCGCAGGACGCCGAGGCCTTCCTGCGCGGGACCGCCGTC
CCCGCGGGCGTGAAGCTGTC

Downstream 100 bases:

>100_bases
CCGAGGGTCGCGCCCGCGCCGCCCGGAGGACCGCCTTGACCCGTACGCTCGTCCTCGCCGCGCTCTGCGCCCTGGCTGCG
GCGCCCGCCGTCGCGGCGGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MMPRYRLLIEYDGRPFNGFQAQDGLPTIQGAIERAVKAFCGEDLRLQAAGRTDTGVHARGQVVHVDLAKDWPAETVRNAL
NAHLVPQPIAVLEASVAPEGWHARFSAKERRYLYRILNRMAPPALDQGRVWHVKKPLDAEAMHEAAQALVGHHDFTTFRD
LGCQAKSPMKTLDLASVRREGDEVLLEFASRSFLHRQVRSMTGTLAEVGVGRWTRADLVAALEARDRRACGPVAPAEGLY
LTGVAY

Sequences:

>Translated_246_residues
MMPRYRLLIEYDGRPFNGFQAQDGLPTIQGAIERAVKAFCGEDLRLQAAGRTDTGVHARGQVVHVDLAKDWPAETVRNAL
NAHLVPQPIAVLEASVAPEGWHARFSAKERRYLYRILNRMAPPALDQGRVWHVKKPLDAEAMHEAAQALVGHHDFTTFRD
LGCQAKSPMKTLDLASVRREGDEVLLEFASRSFLHRQVRSMTGTLAEVGVGRWTRADLVAALEARDRRACGPVAPAEGLY
LTGVAY
>Mature_246_residues
MMPRYRLLIEYDGRPFNGFQAQDGLPTIQGAIERAVKAFCGEDLRLQAAGRTDTGVHARGQVVHVDLAKDWPAETVRNAL
NAHLVPQPIAVLEASVAPEGWHARFSAKERRYLYRILNRMAPPALDQGRVWHVKKPLDAEAMHEAAQALVGHHDFTTFRD
LGCQAKSPMKTLDLASVRREGDEVLLEFASRSFLHRQVRSMTGTLAEVGVGRWTRADLVAALEARDRRACGPVAPAEGLY
LTGVAY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=36.3970588235294, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=42, Blast_Score=172, Evalue=2e-44,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=29.2358803986711, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27272; Mature: 27272

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRYRLLIEYDGRPFNGFQAQDGLPTIQGAIERAVKAFCGEDLRLQAAGRTDTGVHARG
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
QVVHVDLAKDWPAETVRNALNAHLVPQPIAVLEASVAPEGWHARFSAKERRYLYRILNRM
CEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHH
APPALDQGRVWHVKKPLDAEAMHEAAQALVGHHDFTTFRDLGCQAKSPMKTLDLASVRRE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
GDEVLLEFASRSFLHRQVRSMTGTLAEVGVGRWTRADLVAALEARDRRACGPVAPAEGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEE
LTGVAY
EEEECC
>Mature Secondary Structure
MMPRYRLLIEYDGRPFNGFQAQDGLPTIQGAIERAVKAFCGEDLRLQAAGRTDTGVHARG
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
QVVHVDLAKDWPAETVRNALNAHLVPQPIAVLEASVAPEGWHARFSAKERRYLYRILNRM
CEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHH
APPALDQGRVWHVKKPLDAEAMHEAAQALVGHHDFTTFRDLGCQAKSPMKTLDLASVRRE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
GDEVLLEFASRSFLHRQVRSMTGTLAEVGVGRWTRADLVAALEARDRRACGPVAPAEGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEE
LTGVAY
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA