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The map label for this gene is fmt

Identifier: 197103843

GI number: 197103843

Start: 381658

End: 382584

Strand: Direct

Name: fmt

Synonym: PHZ_c0377

Alternate gene names: 197103843

Gene position: 381658-382584 (Clockwise)

Preceding gene: 197103840

Following gene: 197103844

Centisome position: 9.55

GC content: 75.19

Gene sequence:

>927_bases
ATGCGTCTCGCCTTCCTCGGCACCCCCGACTTCGCCGTCCAGGCCCTCGCCGAAATCGTGGAGGCCGGCCACGAGGTGGC
CTGCGTCTATTCCCAACCGCCCGCCCCGCGCGGGCGCGGCCACGAGCTGAGGCCCTCGCCGGTCCACGCCTATGCCGAGA
GCCGTGGCATCCCGGTGCGCACGCCGGCCTCCATGCGCGATCCCGCCGAGATCGAGGCCTTCCGCGCGCTCGGCCTGGAC
GCGGCCGTGGTGGTCGCCTTCGGCCAGATCCTGCCGCGCGAGGTGCTGGAGGCGCCGCGTCTGGGTTCGTTCAACGTCCA
CGCCTCGCTGCTGCCGAGGTGGCGGGGGGCCGCGCCGATCCAGCGGGCGATCATGGCCGGCGACGCCGTCACCGGGGTCC
AGGTGATGCGGATGACCGAGGGGCTGGACGAGGGGCCGGTGCTCTCCACCGCGACGGTGCGGATCGACGCCCTGGAAACC
GCCGCCACCCTGCACGATCGGCTGGCCGCCGCCGGCGCCGGCCTGATCGTCGAGACCCTGGCCCAGATCGCGGCCGGGCG
GGCGGTCGAGACGCCCCAGGCCGAGGCGGGCGTCACCTACGCCAAGAAGATCCGCCCGAAGGAGGCGCGGATCGACTGGA
CGCGCCCGGGTCCCGAGGTGGACCGCAAGATCCGCGGGCTCTCGCCCTTCCCGGGCGCCTGGTTCGAGTTGCCGACCGAG
AAGGGCCCGGTGCGGGTGAAGGCGCTCTTGTCGGCGTTCGAGGACGCCGAGGGGCCGGCGGGCGAGACGCTGGACGACCG
GCTGCTGGTGGGGACCGGGCACGGCGCGGTGCGCCTGCTGCGCGTGCAGCGCGAGGGGCGCGGGCCGCAGGACGCCGAGG
CCTTCCTGCGCGGGACCGCCGTCCCCGCGGGCGTGAAGCTGTCCTGA

Upstream 100 bases:

>100_bases
CTGTCTTCCTCAAGAGTGGCGTCGGGCTGGCGCTGGGGCAGCAACGGCCACGCTTGCGGCGAAGTTCCCTTGAGGCCCCG
GGGCCGCTAAACCGCCGCCC

Downstream 100 bases:

>100_bases
TGCCCCGCTACCGCCTCCTGATCGAATACGACGGCCGGCCGTTCAACGGCTTCCAGGCGCAGGACGGGCTGCCCACGATC
CAGGGCGCCATCGAGCGGGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRLAFLGTPDFAVQALAEIVEAGHEVACVYSQPPAPRGRGHELRPSPVHAYAESRGIPVRTPASMRDPAEIEAFRALGLD
AAVVVAFGQILPREVLEAPRLGSFNVHASLLPRWRGAAPIQRAIMAGDAVTGVQVMRMTEGLDEGPVLSTATVRIDALET
AATLHDRLAAAGAGLIVETLAQIAAGRAVETPQAEAGVTYAKKIRPKEARIDWTRPGPEVDRKIRGLSPFPGAWFELPTE
KGPVRVKALLSAFEDAEGPAGETLDDRLLVGTGHGAVRLLRVQREGRGPQDAEAFLRGTAVPAGVKLS

Sequences:

>Translated_308_residues
MRLAFLGTPDFAVQALAEIVEAGHEVACVYSQPPAPRGRGHELRPSPVHAYAESRGIPVRTPASMRDPAEIEAFRALGLD
AAVVVAFGQILPREVLEAPRLGSFNVHASLLPRWRGAAPIQRAIMAGDAVTGVQVMRMTEGLDEGPVLSTATVRIDALET
AATLHDRLAAAGAGLIVETLAQIAAGRAVETPQAEAGVTYAKKIRPKEARIDWTRPGPEVDRKIRGLSPFPGAWFELPTE
KGPVRVKALLSAFEDAEGPAGETLDDRLLVGTGHGAVRLLRVQREGRGPQDAEAFLRGTAVPAGVKLS
>Mature_308_residues
MRLAFLGTPDFAVQALAEIVEAGHEVACVYSQPPAPRGRGHELRPSPVHAYAESRGIPVRTPASMRDPAEIEAFRALGLD
AAVVVAFGQILPREVLEAPRLGSFNVHASLLPRWRGAAPIQRAIMAGDAVTGVQVMRMTEGLDEGPVLSTATVRIDALET
AATLHDRLAAAGAGLIVETLAQIAAGRAVETPQAEAGVTYAKKIRPKEARIDWTRPGPEVDRKIRGLSPFPGAWFELPTE
KGPVRVKALLSAFEDAEGPAGETLDDRLLVGTGHGAVRLLRVQREGRGPQDAEAFLRGTAVPAGVKLS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=247, Percent_Identity=31.5789473684211, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI238814322, Length=243, Percent_Identity=30.0411522633745, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI164663775, Length=247, Percent_Identity=29.5546558704453, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789683, Length=300, Percent_Identity=44.3333333333333, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1788589, Length=245, Percent_Identity=27.3469387755102, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=255, Percent_Identity=31.3725490196078, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319458, Length=278, Percent_Identity=27.6978417266187, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550868, Length=315, Percent_Identity=30.4761904761905, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571984, Length=267, Percent_Identity=32.2097378277154, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585660, Length=241, Percent_Identity=28.6307053941909, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PHEZH (B4RDU2)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129220.1
- GeneID:   6789867
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0377
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK889816
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32689; Mature: 32689

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAFLGTPDFAVQALAEIVEAGHEVACVYSQPPAPRGRGHELRPSPVHAYAESRGIPVR
CEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
TPASMRDPAEIEAFRALGLDAAVVVAFGQILPREVLEAPRLGSFNVHASLLPRWRGAAPI
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHH
QRAIMAGDAVTGVQVMRMTEGLDEGPVLSTATVRIDALETAATLHDRLAAAGAGLIVETL
HHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHH
AQIAAGRAVETPQAEAGVTYAKKIRPKEARIDWTRPGPEVDRKIRGLSPFPGAWFELPTE
HHHHCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCEECCCC
KGPVRVKALLSAFEDAEGPAGETLDDRLLVGTGHGAVRLLRVQREGRGPQDAEAFLRGTA
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEHHCCCCCHHHHHHHHCCC
VPAGVKLS
CCCCCCCC
>Mature Secondary Structure
MRLAFLGTPDFAVQALAEIVEAGHEVACVYSQPPAPRGRGHELRPSPVHAYAESRGIPVR
CEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
TPASMRDPAEIEAFRALGLDAAVVVAFGQILPREVLEAPRLGSFNVHASLLPRWRGAAPI
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHH
QRAIMAGDAVTGVQVMRMTEGLDEGPVLSTATVRIDALETAATLHDRLAAAGAGLIVETL
HHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHH
AQIAAGRAVETPQAEAGVTYAKKIRPKEARIDWTRPGPEVDRKIRGLSPFPGAWFELPTE
HHHHCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCEECCCC
KGPVRVKALLSAFEDAEGPAGETLDDRLLVGTGHGAVRLLRVQREGRGPQDAEAFLRGTA
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEHHCCCCCHHHHHHHHCCC
VPAGVKLS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA