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The map label for this gene is def

Identifier: 197103839

GI number: 197103839

Start: 377412

End: 377921

Strand: Direct

Name: def

Synonym: PHZ_c0373

Alternate gene names: 197103839

Gene position: 377412-377921 (Clockwise)

Preceding gene: 197103838

Following gene: 197103840

Centisome position: 9.44

GC content: 65.69

Gene sequence:

>510_bases
ATGGCCATCCGAGAGATCCTCGTCGTCCCGCACCCTGCCCTGAAGCAGGTCTCCCAGCCCGTCGAAAAGGTCGACGACGA
GCTGCGCGCGCTGATGGACGACATGCTGGAGACCATGTACGCGGCCCCGGGCATCGGCCTGGCCGCCATCCAGATCGGCG
TGCCCAAGCGCGTGATCGTCATGGACCTGGCCCGGGAGGGCGAGGAGAAGCAGCCCCGCTACTTCGTGAACCCCGAGATC
CTGTGGGCCTCCGACGATACCGCGCCGTACGAGGAGGGCTGCCTGTCGGTTCCCGAGTACTACGACGAGGTCGAGCGGCC
GGCGCGCGTGAAGCTGCGCTACCTGAACTACCAGGGCGAACAGGTCGAGGAGGACGCCGAGGGCCTCTTCGCGGTCTGTA
TCCAGCACGAGATGGACCACCTGGAAGGCGTCCTGTTCATCGACCACCTGTCGCGCCTGAAGCGCGAGCAGGCGATCAAG
AAGGTGAAGAAGCACGCCAAAGCGGCCTAA

Upstream 100 bases:

>100_bases
GACGAGCCTCAGCCCAGCGTGCGGCTCGCCGCCGCCGGCAAGCCCGACTGAGGCCTTCTCCCACCTTGACGCTCAGGCAT
TCCGAACCTACCTCGCGCCC

Downstream 100 bases:

>100_bases
AAGCCGCTTCGTGGCCAAGCATCCGCTCATCCGTTTCGTCCGCCGGGGCCTCGAGGTCACCGGCCTGATCGCGCCCATCT
ACCGGGCGAAGGAACGCAAG

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MAIREILVVPHPALKQVSQPVEKVDDELRALMDDMLETMYAAPGIGLAAIQIGVPKRVIVMDLAREGEEKQPRYFVNPEI
LWASDDTAPYEEGCLSVPEYYDEVERPARVKLRYLNYQGEQVEEDAEGLFAVCIQHEMDHLEGVLFIDHLSRLKREQAIK
KVKKHAKAA

Sequences:

>Translated_169_residues
MAIREILVVPHPALKQVSQPVEKVDDELRALMDDMLETMYAAPGIGLAAIQIGVPKRVIVMDLAREGEEKQPRYFVNPEI
LWASDDTAPYEEGCLSVPEYYDEVERPARVKLRYLNYQGEQVEEDAEGLFAVCIQHEMDHLEGVLFIDHLSRLKREQAIK
KVKKHAKAA
>Mature_168_residues
AIREILVVPHPALKQVSQPVEKVDDELRALMDDMLETMYAAPGIGLAAIQIGVPKRVIVMDLAREGEEKQPRYFVNPEIL
WASDDTAPYEEGCLSVPEYYDEVERPARVKLRYLNYQGEQVEEDAEGLFAVCIQHEMDHLEGVLFIDHLSRLKREQAIKK
VKKHAKAA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=156, Percent_Identity=35.8974358974359, Blast_Score=85, Evalue=3e-17,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=46.3414634146341, Blast_Score=148, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=32.7272727272727, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=35.4430379746835, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PHEZH (B4RDT8)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129216.1
- GeneID:   6789863
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0373
- HOGENOM:   HBG665227
- OMA:   AADMAET
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19255; Mature: 19124

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIREILVVPHPALKQVSQPVEKVDDELRALMDDMLETMYAAPGIGLAAIQIGVPKRVIV
CCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
MDLAREGEEKQPRYFVNPEILWASDDTAPYEEGCLSVPEYYDEVERPARVKLRYLNYQGE
HHHHHCCCCCCCCEEECCEEEEECCCCCCHHHHCCCCHHHHHHHCCCHHEEEEEECCCCC
QVEEDAEGLFAVCIQHEMDHLEGVLFIDHLSRLKREQAIKKVKKHAKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIREILVVPHPALKQVSQPVEKVDDELRALMDDMLETMYAAPGIGLAAIQIGVPKRVIV
CCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
MDLAREGEEKQPRYFVNPEILWASDDTAPYEEGCLSVPEYYDEVERPARVKLRYLNYQGE
HHHHHCCCCCCCCEEECCEEEEECCCCCCHHHHCCCCHHHHHHHCCCHHEEEEEECCCCC
QVEEDAEGLFAVCIQHEMDHLEGVLFIDHLSRLKREQAIKKVKKHAKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA