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The map label for this gene is hisS [H]

Identifier: 197103798

GI number: 197103798

Start: 328941

End: 330458

Strand: Direct

Name: hisS [H]

Synonym: PHZ_c0332

Alternate gene names: 197103798

Gene position: 328941-330458 (Clockwise)

Preceding gene: 197103797

Following gene: 197103799

Centisome position: 8.23

GC content: 73.98

Gene sequence:

>1518_bases
TTGAGCGCGCAGGCCGCAGCCGGTAAGCGCCCAGCCATGGCCCAAGACCCGCAACGCCCCGAAGCCCGCACGCCGCGCGG
CTTCGTCGACCGCCGAGCCCGCGACGTGGTCGCCGAGCGGCAGATCCTGACCGCCGTCTCAGGAGTCTATGAGCGCTGGG
GCTTCGAGCCGCTGGAGACCGGGGCGTTCGAATACGCCGACGCCCTGGGCAAGTTCCTGCCCGACGCCGACCGTCCGAAC
GAGGGCGTCTTCGCCCTGCAGGACGACGACGAGCAGTGGATGGCGCTGCGCTACGACCTGACTGCGCCGCTGGCCCGCTT
CGCCGCCCAGAACTGGGAGACCCTGCCCAAGCCGTTCCGCCGCTACGCCTACGGGCCGGTCTGGCGCAACGAGAAGCCGG
GGCCGGGGCGCTTCCGCGAGTTCGTCCAGTGCGACGCCGACACGGTGGGCTCGGCCCGTCCCGAGGCCGACGCCGAGATC
ATCGCCATGGCCGTGCAGGGCCTGGAGGCCGCGGGCCTGCCGGCCGGCTCGGCGGTCATCAAGATCAACAACCGCAAGCT
GCTGAACGGCCTGATGGCGGCGGCCGGGGTGACCGACGAGGCCCAGAAGCTCGGCGTGCTGCGCGCGGTCGACAAGCTGG
ACCGCCTGGGCGCGGACGGCGTGCGCCTGCTGCTGGGCGAGGGCCGCCTCGACGAGTCCGGCGCCTTCACCAAGGGGGCG
GGGCTCTCGGCCCAGTCCGCCGAACGGGTGCTGGCCTTCACCGCCGCCGGCGTCGGCGGCCGGGCCGAAACCCTGGCCAA
GCTGGCCGAGGTCGTCGGCGGCTCGGCCGAGGGCGACGAGGGCCTGGCCGAGCTGGCCGCCATCGACGCGGCCCTGTCGG
CGCTCGGCGTCGGGCCCGACCGGGCGCTGTTCGACCCGGCCGTCGTGCGGGGCCTGGAGTACTACACGGGCGCGGTGTTC
GAGGCCGAGCTCTTGCTCTCCACCACGGACGAGAAAGGACAGAAGGTCACCTTCGGCTCGATCGGCGGCGGCGGGCGCTA
CGATGACCTCGTGGCCCGCTTCACCGGCGAGCGGACGCCCGCCACCGGCTTCTCCTTCGGCGTCTCGCGCCTGGCCGCGG
CCCTGCGCGCCGCCGGCCGTGACGCAGCCCGAAAGGGTCCCTGGGCCGTCCGCGGCCCGGTGGTGGTCATCGCCTTCGAC
CAGGCGCGCATGGCCGACTACTTCGCCCTGGCCGGCGAGATCCGCGCGGCGGGCGTCCCGGCGGAGGTCTACCTGGGAAG
CTCGGGCATGAAGGCCCAGATGAAGTACGCCGACCGGCGCCTGTCGCCCGCGGCGGTCATCCTGGGCGAGGACGAGATCG
CCGCCGGCACGGTCACCGTGAAGGACCTCGACCTCGGCCGCGAGCTGGCCAGCAAGGTGACCGACAACGCCGAGTGGCGC
GAGCAGCGGCCCGGCCAGCAGACCATCGCCCGCGCCGAGCTGATCCCCGCCCTGCGCCGCATCCTGGACGCCGGCTGA

Upstream 100 bases:

>100_bases
AGCCCCGCCGATGCGGACGGGGCTGTTCTTCTGACAAGCGCCGTCCGTCCCTCAGACTTCCCGAGAGACGACGATGACCG
AACCTTCCCCAACTGCCTCC

Downstream 100 bases:

>100_bases
TGCGCGTCGAGCCGCCCGTGCCGGAGGCGGCGCTCGCCGCCATCCGCGCGCCCCTGGAGGCCGCCGGCGCGACTCGCGCC
GACGCGCCCCTGCTGCAGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSAQAAAGKRPAMAQDPQRPEARTPRGFVDRRARDVVAERQILTAVSGVYERWGFEPLETGAFEYADALGKFLPDADRPN
EGVFALQDDDEQWMALRYDLTAPLARFAAQNWETLPKPFRRYAYGPVWRNEKPGPGRFREFVQCDADTVGSARPEADAEI
IAMAVQGLEAAGLPAGSAVIKINNRKLLNGLMAAAGVTDEAQKLGVLRAVDKLDRLGADGVRLLLGEGRLDESGAFTKGA
GLSAQSAERVLAFTAAGVGGRAETLAKLAEVVGGSAEGDEGLAELAAIDAALSALGVGPDRALFDPAVVRGLEYYTGAVF
EAELLLSTTDEKGQKVTFGSIGGGGRYDDLVARFTGERTPATGFSFGVSRLAAALRAAGRDAARKGPWAVRGPVVVIAFD
QARMADYFALAGEIRAAGVPAEVYLGSSGMKAQMKYADRRLSPAAVILGEDEIAAGTVTVKDLDLGRELASKVTDNAEWR
EQRPGQQTIARAELIPALRRILDAG

Sequences:

>Translated_505_residues
MSAQAAAGKRPAMAQDPQRPEARTPRGFVDRRARDVVAERQILTAVSGVYERWGFEPLETGAFEYADALGKFLPDADRPN
EGVFALQDDDEQWMALRYDLTAPLARFAAQNWETLPKPFRRYAYGPVWRNEKPGPGRFREFVQCDADTVGSARPEADAEI
IAMAVQGLEAAGLPAGSAVIKINNRKLLNGLMAAAGVTDEAQKLGVLRAVDKLDRLGADGVRLLLGEGRLDESGAFTKGA
GLSAQSAERVLAFTAAGVGGRAETLAKLAEVVGGSAEGDEGLAELAAIDAALSALGVGPDRALFDPAVVRGLEYYTGAVF
EAELLLSTTDEKGQKVTFGSIGGGGRYDDLVARFTGERTPATGFSFGVSRLAAALRAAGRDAARKGPWAVRGPVVVIAFD
QARMADYFALAGEIRAAGVPAEVYLGSSGMKAQMKYADRRLSPAAVILGEDEIAAGTVTVKDLDLGRELASKVTDNAEWR
EQRPGQQTIARAELIPALRRILDAG
>Mature_504_residues
SAQAAAGKRPAMAQDPQRPEARTPRGFVDRRARDVVAERQILTAVSGVYERWGFEPLETGAFEYADALGKFLPDADRPNE
GVFALQDDDEQWMALRYDLTAPLARFAAQNWETLPKPFRRYAYGPVWRNEKPGPGRFREFVQCDADTVGSARPEADAEII
AMAVQGLEAAGLPAGSAVIKINNRKLLNGLMAAAGVTDEAQKLGVLRAVDKLDRLGADGVRLLLGEGRLDESGAFTKGAG
LSAQSAERVLAFTAAGVGGRAETLAKLAEVVGGSAEGDEGLAELAAIDAALSALGVGPDRALFDPAVVRGLEYYTGAVFE
AELLLSTTDEKGQKVTFGSIGGGGRYDDLVARFTGERTPATGFSFGVSRLAAALRAAGRDAARKGPWAVRGPVVVIAFDQ
ARMADYFALAGEIRAAGVPAEVYLGSSGMKAQMKYADRRLSPAAVILGEDEIAAGTVTVKDLDLGRELASKVTDNAEWRE
QRPGQQTIARAELIPALRRILDAG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=457, Percent_Identity=29.7592997811816, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI6996014, Length=484, Percent_Identity=28.7190082644628, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1788861, Length=398, Percent_Identity=26.3819095477387, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71993686, Length=481, Percent_Identity=30.1455301455301, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71993693, Length=481, Percent_Identity=30.1455301455301, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325290, Length=480, Percent_Identity=28.9583333333333, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24643061, Length=487, Percent_Identity=30.5954825462012, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24643059, Length=506, Percent_Identity=29.2490118577075, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 53594; Mature: 53463

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQAAAGKRPAMAQDPQRPEARTPRGFVDRRARDVVAERQILTAVSGVYERWGFEPLET
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GAFEYADALGKFLPDADRPNEGVFALQDDDEQWMALRYDLTAPLARFAAQNWETLPKPFR
CHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHCCCHHCCHHHH
RYAYGPVWRNEKPGPGRFREFVQCDADTVGSARPEADAEIIAMAVQGLEAAGLPAGSAVI
HHCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHCHHHCCCCCCCEEE
KINNRKLLNGLMAAAGVTDEAQKLGVLRAVDKLDRLGADGVRLLLGEGRLDESGAFTKGA
EECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCC
GLSAQSAERVLAFTAAGVGGRAETLAKLAEVVGGSAEGDEGLAELAAIDAALSALGVGPD
CCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
RALFDPAVVRGLEYYTGAVFEAELLLSTTDEKGQKVTFGSIGGGGRYDDLVARFTGERTP
HHHHCHHHHHHHHHHHCHHEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
ATGFSFGVSRLAAALRAAGRDAARKGPWAVRGPVVVIAFDQARMADYFALAGEIRAAGVP
CCCHHHHHHHHHHHHHHHCCHHHCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHCCCC
AEVYLGSSGMKAQMKYADRRLSPAAVILGEDEIAAGTVTVKDLDLGRELASKVTDNAEWR
CEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHH
EQRPGQQTIARAELIPALRRILDAG
HCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAQAAAGKRPAMAQDPQRPEARTPRGFVDRRARDVVAERQILTAVSGVYERWGFEPLET
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GAFEYADALGKFLPDADRPNEGVFALQDDDEQWMALRYDLTAPLARFAAQNWETLPKPFR
CHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHCCCHHCCHHHH
RYAYGPVWRNEKPGPGRFREFVQCDADTVGSARPEADAEIIAMAVQGLEAAGLPAGSAVI
HHCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHCHHHCCCCCCCEEE
KINNRKLLNGLMAAAGVTDEAQKLGVLRAVDKLDRLGADGVRLLLGEGRLDESGAFTKGA
EECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCC
GLSAQSAERVLAFTAAGVGGRAETLAKLAEVVGGSAEGDEGLAELAAIDAALSALGVGPD
CCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
RALFDPAVVRGLEYYTGAVFEAELLLSTTDEKGQKVTFGSIGGGGRYDDLVARFTGERTP
HHHHCHHHHHHHHHHHCHHEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
ATGFSFGVSRLAAALRAAGRDAARKGPWAVRGPVVVIAFDQARMADYFALAGEIRAAGVP
CCCHHHHHHHHHHHHHHHCCHHHCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHCCCC
AEVYLGSSGMKAQMKYADRRLSPAAVILGEDEIAAGTVTVKDLDLGRELASKVTDNAEWR
CEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHH
EQRPGQQTIARAELIPALRRILDAG
HCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]