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The map label for this gene is kefB [H]

Identifier: 197103784

GI number: 197103784

Start: 310723

End: 312582

Strand: Direct

Name: kefB [H]

Synonym: PHZ_c0318

Alternate gene names: 197103784

Gene position: 310723-312582 (Clockwise)

Preceding gene: 197103782

Following gene: 197103785

Centisome position: 7.78

GC content: 72.69

Gene sequence:

>1860_bases
ATGGCGGACGCGGCGGCGGGCATGGACCTGGGGCACGGGGTGGCCCTGCTGGCGGCCGCGGTGGTCGCCGTCCCGATCTT
CCGCAAGCTCGGCCTGGGCTCGGTCCTGGGCTACCTCGCCGCGGGCCTGGCCATCGGGCCGTTCGGCCTGCGGCTCTTCG
CCGATCCCGAGGCGATCCTGCACTTCGCCGAGCTCGGGGTGGTGCTGTTCCTGTTCCTGATCGGGCTGGAGATGCGGCCG
GCGCGGCTCTGGAGCCTGCGCAAGGAGATCTTCGGCCTGGGCGCGACCCAGGTGCTGGCCTGCTGCGCCCTGCTGTCGGG
GATCGCGCTGCTGGCGGGCCTGCCGTGGCAGGCGGCGGTGATCGGCGCCAGCGGCTTCGTGCTGTCGTCCACCGCGGTGA
TCATGAAGATGCTGGACGAGCAGGGCGAGACCGCCACCGAGGCGGGTCAGCGGGCGATCTCGATCCTGCTGCTGGAGGAC
ATCGCCGTCGTCCCGCTGCTGGCCCTGGTGGCCGTCCTGGCCGCCGCGTCCGGCTACGCCGACGCCGACGCCCGGCCCGC
CTGGCAGGCCCTCGCCGTCGCCGCCGGCTGCATCGCCGTGGTGGTGGCCGCCGGCAAGTGGCTGCTCAACCCGTTCTTCC
GGGTGCTGGCCCGCTCGGGCGCGCGGGAGATCATGACCGCCGCCGCCCTGCTGGTGGTGCTGGGGACGGCGCTGTTCATG
CAGTGGGGCGGCCTCTCCATGGCGATGGGCGCCTTCCTGGCCGGCGTCCTGCTGTCGGAGAGCGTCTTCCGCCATCAGCT
GGAGGCCGACATCGAGCCGTTCCGCGGCATCCTGCTGGGCCTGTTCTTCCTCAGCGTCGGCATGTCGCTGGACGTCGCGG
TGGTGGTCGCCGAGTGGGGCCTGGTGGTCGCGGGCCTGGCCGCCTTCATGGTCGCCAAGGCCGCCGGCATCTATCTCGTG
GCCCGCCTGTTCCGCGCCCCGCCGCGCGAGGCGGTCCAGCGCGCGGCCCTGTTCGCCCAAGGGGGCGAGTTCGCCTTCGT
CCTCTACGGCGCGGCGACGGCCGCGGGGGTGATGGCGCCTCGGGTCGGCGCCCTGATGAGCGCCGTCGTCATCCTCTCGA
TGGCGGTGACCCCGCTGGTGGTGATCCTGCTGAACCGGCTCATGCCCCCGCCGAAGGTCTCCATGGACGGGGTGGAGGCG
CCCGACGGCCTGGAGGGCCGCGTCCTGCTGATCGGCTTCGGCCGCTTCGCCCAGGTGGTCAGCCAGCCGCTGCTGGCCAA
GGGCTTCGAGATCTCGCTGATCGAATCCGACGTGGAGATGATCCGCGCGGCCGGATCGTTCGGCTTCAAGGTCTACTACG
GCGACGGCACCCGCCTGGACGTGCTGCGCGCCTCGGGCGCGGAGCGGGCCGAGGCGATCCTGGTATGCGTCGACAAGCCC
GACGCGGCCGATCGGATCGTCGAGCTGGTCAAGGCCGAGTTCCCGCTCGCCAAGGTGTACGCCCGCGCCTACGACCGCGG
CCACTCCATGCGGCTGGTCCAGGCCGGCGTCGATTTCCAGATCCGCGAGACCTTCGAGTCCGCCATCAAGATGGGCCGCC
ACGTCCTGCACGGCCTGGGCGTGGACGAGGCCGAGGCGGCCGAGATCATCCAGGACGTGCGCGAGCGCGACAACGAGCGC
TTCCAGCTCCAGCTCACCGGCGGGCTCGCCGCCGGCCGCTACCTGATGCGCGGCAACATGCCGACCCCGACGCCCGAGCC
GTTCTCGGTCCCCCGCCGCGGCGGCGAGGCGCTCAACCCCGAAGCGGCCGAGGCGCTGGCCCAGCGCAAGGCGGGCCGCG
TTCCGCACAAGGCGAAGTAG

Upstream 100 bases:

>100_bases
CGCGCCTCCAGCGGGCGCGGCGATCAGCCCTTCGCCCGCTCGCGTGTTGCCTCCCGGGGGCGTTAGCCGCCACATAGCGC
GGCGGGGAGCAGGAGCGGCG

Downstream 100 bases:

>100_bases
GATCCCTCGGCGAGGCGGCTGCGTCCCAGGCTCCGAGCGGGGCCTTTGTTCATCGATCGATGAATTTTTTGGTCGACCCC
GGTTCTCAACCGGACGTTTG

Product: glutathione-regulated potassium efflux protein B

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MADAAAGMDLGHGVALLAAAVVAVPIFRKLGLGSVLGYLAAGLAIGPFGLRLFADPEAILHFAELGVVLFLFLIGLEMRP
ARLWSLRKEIFGLGATQVLACCALLSGIALLAGLPWQAAVIGASGFVLSSTAVIMKMLDEQGETATEAGQRAISILLLED
IAVVPLLALVAVLAAASGYADADARPAWQALAVAAGCIAVVVAAGKWLLNPFFRVLARSGAREIMTAAALLVVLGTALFM
QWGGLSMAMGAFLAGVLLSESVFRHQLEADIEPFRGILLGLFFLSVGMSLDVAVVVAEWGLVVAGLAAFMVAKAAGIYLV
ARLFRAPPREAVQRAALFAQGGEFAFVLYGAATAAGVMAPRVGALMSAVVILSMAVTPLVVILLNRLMPPPKVSMDGVEA
PDGLEGRVLLIGFGRFAQVVSQPLLAKGFEISLIESDVEMIRAAGSFGFKVYYGDGTRLDVLRASGAERAEAILVCVDKP
DAADRIVELVKAEFPLAKVYARAYDRGHSMRLVQAGVDFQIRETFESAIKMGRHVLHGLGVDEAEAAEIIQDVRERDNER
FQLQLTGGLAAGRYLMRGNMPTPTPEPFSVPRRGGEALNPEAAEALAQRKAGRVPHKAK

Sequences:

>Translated_619_residues
MADAAAGMDLGHGVALLAAAVVAVPIFRKLGLGSVLGYLAAGLAIGPFGLRLFADPEAILHFAELGVVLFLFLIGLEMRP
ARLWSLRKEIFGLGATQVLACCALLSGIALLAGLPWQAAVIGASGFVLSSTAVIMKMLDEQGETATEAGQRAISILLLED
IAVVPLLALVAVLAAASGYADADARPAWQALAVAAGCIAVVVAAGKWLLNPFFRVLARSGAREIMTAAALLVVLGTALFM
QWGGLSMAMGAFLAGVLLSESVFRHQLEADIEPFRGILLGLFFLSVGMSLDVAVVVAEWGLVVAGLAAFMVAKAAGIYLV
ARLFRAPPREAVQRAALFAQGGEFAFVLYGAATAAGVMAPRVGALMSAVVILSMAVTPLVVILLNRLMPPPKVSMDGVEA
PDGLEGRVLLIGFGRFAQVVSQPLLAKGFEISLIESDVEMIRAAGSFGFKVYYGDGTRLDVLRASGAERAEAILVCVDKP
DAADRIVELVKAEFPLAKVYARAYDRGHSMRLVQAGVDFQIRETFESAIKMGRHVLHGLGVDEAEAAEIIQDVRERDNER
FQLQLTGGLAAGRYLMRGNMPTPTPEPFSVPRRGGEALNPEAAEALAQRKAGRVPHKAK
>Mature_618_residues
ADAAAGMDLGHGVALLAAAVVAVPIFRKLGLGSVLGYLAAGLAIGPFGLRLFADPEAILHFAELGVVLFLFLIGLEMRPA
RLWSLRKEIFGLGATQVLACCALLSGIALLAGLPWQAAVIGASGFVLSSTAVIMKMLDEQGETATEAGQRAISILLLEDI
AVVPLLALVAVLAAASGYADADARPAWQALAVAAGCIAVVVAAGKWLLNPFFRVLARSGAREIMTAAALLVVLGTALFMQ
WGGLSMAMGAFLAGVLLSESVFRHQLEADIEPFRGILLGLFFLSVGMSLDVAVVVAEWGLVVAGLAAFMVAKAAGIYLVA
RLFRAPPREAVQRAALFAQGGEFAFVLYGAATAAGVMAPRVGALMSAVVILSMAVTPLVVILLNRLMPPPKVSMDGVEAP
DGLEGRVLLIGFGRFAQVVSQPLLAKGFEISLIESDVEMIRAAGSFGFKVYYGDGTRLDVLRASGAERAEAILVCVDKPD
AADRIVELVKAEFPLAKVYARAYDRGHSMRLVQAGVDFQIRETFESAIKMGRHVLHGLGVDEAEAAEIIQDVRERDNERF
QLQLTGGLAAGRYLMRGNMPTPTPEPFSVPRRGGEALNPEAAEALAQRKAGRVPHKAK

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=393, Percent_Identity=28.2442748091603, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1786232, Length=556, Percent_Identity=44.4244604316547, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789749, Length=551, Percent_Identity=43.557168784029, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786685, Length=500, Percent_Identity=30.2, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65185; Mature: 65053

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00664 VINCULIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAAAGMDLGHGVALLAAAVVAVPIFRKLGLGSVLGYLAAGLAIGPFGLRLFADPEAIL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCHHHHH
HFAELGVVLFLFLIGLEMRPARLWSLRKEIFGLGATQVLACCALLSGIALLAGLPWQAAV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHE
IGASGFVLSSTAVIMKMLDEQGETATEAGQRAISILLLEDIAVVPLLALVAVLAAASGYA
ECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DADARPAWQALAVAAGCIAVVVAAGKWLLNPFFRVLARSGAREIMTAAALLVVLGTALFM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QWGGLSMAMGAFLAGVLLSESVFRHQLEADIEPFRGILLGLFFLSVGMSLDVAVVVAEWG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LVVAGLAAFMVAKAAGIYLVARLFRAPPREAVQRAALFAQGGEFAFVLYGAATAAGVMAP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
RVGALMSAVVILSMAVTPLVVILLNRLMPPPKVSMDGVEAPDGLEGRVLLIGFGRFAQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHH
SQPLLAKGFEISLIESDVEMIRAAGSFGFKVYYGDGTRLDVLRASGAERAEAILVCVDKP
CCHHHHCCCEEEEEHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCC
DAADRIVELVKAEFPLAKVYARAYDRGHSMRLVQAGVDFQIRETFESAIKMGRHVLHGLG
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
VDEAEAAEIIQDVRERDNERFQLQLTGGLAAGRYLMRGNMPTPTPEPFSVPRRGGEALNP
CCHHHHHHHHHHHHHCCCCEEEEEEECCHHHCHHHEECCCCCCCCCCCCCCCCCCCCCCH
EAAEALAQRKAGRVPHKAK
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADAAAGMDLGHGVALLAAAVVAVPIFRKLGLGSVLGYLAAGLAIGPFGLRLFADPEAIL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCHHHHH
HFAELGVVLFLFLIGLEMRPARLWSLRKEIFGLGATQVLACCALLSGIALLAGLPWQAAV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHE
IGASGFVLSSTAVIMKMLDEQGETATEAGQRAISILLLEDIAVVPLLALVAVLAAASGYA
ECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DADARPAWQALAVAAGCIAVVVAAGKWLLNPFFRVLARSGAREIMTAAALLVVLGTALFM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QWGGLSMAMGAFLAGVLLSESVFRHQLEADIEPFRGILLGLFFLSVGMSLDVAVVVAEWG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LVVAGLAAFMVAKAAGIYLVARLFRAPPREAVQRAALFAQGGEFAFVLYGAATAAGVMAP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
RVGALMSAVVILSMAVTPLVVILLNRLMPPPKVSMDGVEAPDGLEGRVLLIGFGRFAQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHH
SQPLLAKGFEISLIESDVEMIRAAGSFGFKVYYGDGTRLDVLRASGAERAEAILVCVDKP
CCHHHHCCCEEEEEHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCC
DAADRIVELVKAEFPLAKVYARAYDRGHSMRLVQAGVDFQIRETFESAIKMGRHVLHGLG
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
VDEAEAAEIIQDVRERDNERFQLQLTGGLAAGRYLMRGNMPTPTPEPFSVPRRGGEALNP
CCHHHHHHHHHHHHHCCCCEEEEEEECCHHHCHHHEECCCCCCCCCCCCCCCCCCCCCCH
EAAEALAQRKAGRVPHKAK
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]