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The map label for this gene is folC [H]

Identifier: 197103664

GI number: 197103664

Start: 190956

End: 192290

Strand: Direct

Name: folC [H]

Synonym: PHZ_c0198

Alternate gene names: 197103664

Gene position: 190956-192290 (Clockwise)

Preceding gene: 197103663

Following gene: 197103672

Centisome position: 4.78

GC content: 74.16

Gene sequence:

>1335_bases
ATGACGTCCGCCGACCCCGGTTTCGACCCGATCCGCGCCTCGGACGCGGTGATCCAGCGGCTGCGCCGCAGCCACCCCAC
GCTCATCGACCTGACGACGGGCCGCGTCGAGCGCCTGCTGGCGGCGCTGGACCATCCCGAACGGCGGCTTCCGCCGGTGA
TCCACGTGGCCGGCACCAACGGCAAGGGCTCGACCGTCGCCTACCTGCGCGCCATCGGCGAGGCGGCGGGCCTGCGGGTC
CACACCCTGACCTCGCCCCACCTCGTGCGGTTCGCCGAGCGGATCCGGCTGGCGGGCCGGCTGATCAGCGACGAGCAGCT
CCTGGCCCTCACCGAGCGGGTCGAGGCGGCCAATGCGGACCAGCCGATCAGCTTCTTCGAGATCACGACGGTGCTGGCCC
TGCTGGCCTTCGCCGAGACGCCGGCCGACCTGTGCGTGGTCGAGGTGGGGCTGGGCGGCCGGTTCGACGCCACCAACGTC
TTCGACGCCCCGGCGGTCAGCGTCATCACGCCCGTGGACTACGACCACCTGGAGATGCTGGGCCCCGGCCTCGCCAAGAT
CGCCTGGGAGAAGGCCGGGATCATCAAGCGCGGGCGGCCGGTGGTGGTGGCGCGCCAGCTGGACGAGGCGCTGGAGATGA
TCGAGCGCGAGGCCGACGACCGCATGGCGCCGATGCTGCTGATGGGCCGCGACTTCGACGCCTGGGAGGAGCGCGGCCGG
CTGCTGGTGCAGATGCCCGAGCGGCTGCTGGACCTGCCGCCGCCCAGCCTGTTCGGCGGCTACCAGTTCGCCAACGCGGG
CCTGGCCGTCGCCGCGGCCCTGACCTTCGACCACGACCTCCCGGACGAGGTGCTGGGCGACGGCGTCGCCTCGGCCGTCT
GGCCGGCGCGGATGCAGCGGCTGACCGCCGGGCCCCTGTTCGAGCTGGCGCGCGCCCGCGGCGCCGACATCTGGCTGGAC
GGCGGCCACAACCCGCATGCGGGCCGCGCGCTCGCCGAGGCCGCCTCGCGCATCGTCGACCGCGACCCGCGGCCGCTGAC
GCTGGTGGCGGGCATGTTCGCCCGCAAGGACGCCGAGGGCTTCTTCCGCCCCTTCGCCGAGATGAAGCCGCGGGTGATCG
CCACCACCTTCGACTCGCCCCAGGCGGCGAGCGCCGAGGACATCGCCGTGGCGGCGGTGAAGGCGGGCCTGCCGGCCGAG
ATGTCGCCGAACGTCGAGGACGCCGTGCGGCGGGCGCTTTCGGTGGACGGGCCCGCGCCGCACGTGCTGGTCTGCGGCGG
CCTGCATTTCGCGGGCGAGGTGCTCGCCATGAGCCCGGAGACCTGGCCCGCCTGA

Upstream 100 bases:

>100_bases
GAAGACGCTTATGATGGGGCGTGAACGCCTTTCCCCCGCCGCCTAGGTCCAAGCCGAGCCTGACCCCCGCTCCCCCCATC
GGAGAGGACGGGATAGGGCC

Downstream 100 bases:

>100_bases
TGCGTGCAAAGCCCTACAGCCTGCGCTTCGCCCTGCCGCGGCACTACCTGATCACCCTGGCGATCGCCCTGGTGGCGCTG
TGGGGACTGGTGGAGGTGTT

Product: bifunctional dihydrofolate synthase/folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTSADPGFDPIRASDAVIQRLRRSHPTLIDLTTGRVERLLAALDHPERRLPPVIHVAGTNGKGSTVAYLRAIGEAAGLRV
HTLTSPHLVRFAERIRLAGRLISDEQLLALTERVEAANADQPISFFEITTVLALLAFAETPADLCVVEVGLGGRFDATNV
FDAPAVSVITPVDYDHLEMLGPGLAKIAWEKAGIIKRGRPVVVARQLDEALEMIEREADDRMAPMLLMGRDFDAWEERGR
LLVQMPERLLDLPPPSLFGGYQFANAGLAVAAALTFDHDLPDEVLGDGVASAVWPARMQRLTAGPLFELARARGADIWLD
GGHNPHAGRALAEAASRIVDRDPRPLTLVAGMFARKDAEGFFRPFAEMKPRVIATTFDSPQAASAEDIAVAAVKAGLPAE
MSPNVEDAVRRALSVDGPAPHVLVCGGLHFAGEVLAMSPETWPA

Sequences:

>Translated_444_residues
MTSADPGFDPIRASDAVIQRLRRSHPTLIDLTTGRVERLLAALDHPERRLPPVIHVAGTNGKGSTVAYLRAIGEAAGLRV
HTLTSPHLVRFAERIRLAGRLISDEQLLALTERVEAANADQPISFFEITTVLALLAFAETPADLCVVEVGLGGRFDATNV
FDAPAVSVITPVDYDHLEMLGPGLAKIAWEKAGIIKRGRPVVVARQLDEALEMIEREADDRMAPMLLMGRDFDAWEERGR
LLVQMPERLLDLPPPSLFGGYQFANAGLAVAAALTFDHDLPDEVLGDGVASAVWPARMQRLTAGPLFELARARGADIWLD
GGHNPHAGRALAEAASRIVDRDPRPLTLVAGMFARKDAEGFFRPFAEMKPRVIATTFDSPQAASAEDIAVAAVKAGLPAE
MSPNVEDAVRRALSVDGPAPHVLVCGGLHFAGEVLAMSPETWPA
>Mature_443_residues
TSADPGFDPIRASDAVIQRLRRSHPTLIDLTTGRVERLLAALDHPERRLPPVIHVAGTNGKGSTVAYLRAIGEAAGLRVH
TLTSPHLVRFAERIRLAGRLISDEQLLALTERVEAANADQPISFFEITTVLALLAFAETPADLCVVEVGLGGRFDATNVF
DAPAVSVITPVDYDHLEMLGPGLAKIAWEKAGIIKRGRPVVVARQLDEALEMIEREADDRMAPMLLMGRDFDAWEERGRL
LVQMPERLLDLPPPSLFGGYQFANAGLAVAAALTFDHDLPDEVLGDGVASAVWPARMQRLTAGPLFELARARGADIWLDG
GHNPHAGRALAEAASRIVDRDPRPLTLVAGMFARKDAEGFFRPFAEMKPRVIATTFDSPQAASAEDIAVAAVKAGLPAEM
SPNVEDAVRRALSVDGPAPHVLVCGGLHFAGEVLAMSPETWPA

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=328, Percent_Identity=30.1829268292683, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI66932990, Length=328, Percent_Identity=30.1829268292683, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1788654, Length=426, Percent_Identity=30.5164319248826, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=365, Percent_Identity=28.2191780821918, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=365, Percent_Identity=28.2191780821918, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=365, Percent_Identity=28.2191780821918, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=428, Percent_Identity=28.7383177570093, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324815, Length=453, Percent_Identity=24.7240618101545, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24641571, Length=380, Percent_Identity=27.6315789473684, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24581568, Length=207, Percent_Identity=31.4009661835749, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47789; Mature: 47658

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSADPGFDPIRASDAVIQRLRRSHPTLIDLTTGRVERLLAALDHPERRLPPVIHVAGTN
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCHHHCCCEEEEECCC
GKGSTVAYLRAIGEAAGLRVHTLTSPHLVRFAERIRLAGRLISDEQLLALTERVEAANAD
CCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
QPISFFEITTVLALLAFAETPADLCVVEVGLGGRFDATNVFDAPAVSVITPVDYDHLEML
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHH
GPGLAKIAWEKAGIIKRGRPVVVARQLDEALEMIEREADDRMAPMLLMGRDFDAWEERGR
CCCHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHHHCHHHCCHHEECCCCHHHHHCCC
LLVQMPERLLDLPPPSLFGGYQFANAGLAVAAALTFDHDLPDEVLGDGVASAVWPARMQR
EEEECHHHHHCCCCCHHHCCCHHHCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHH
LTAGPLFELARARGADIWLDGGHNPHAGRALAEAASRIVDRDPRPLTLVAGMFARKDAEG
HCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHH
FFRPFAEMKPRVIATTFDSPQAASAEDIAVAAVKAGLPAEMSPNVEDAVRRALSVDGPAP
HHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCC
HVLVCGGLHFAGEVLAMSPETWPA
EEEEECCHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
TSADPGFDPIRASDAVIQRLRRSHPTLIDLTTGRVERLLAALDHPERRLPPVIHVAGTN
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCHHHCCCEEEEECCC
GKGSTVAYLRAIGEAAGLRVHTLTSPHLVRFAERIRLAGRLISDEQLLALTERVEAANAD
CCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
QPISFFEITTVLALLAFAETPADLCVVEVGLGGRFDATNVFDAPAVSVITPVDYDHLEML
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHH
GPGLAKIAWEKAGIIKRGRPVVVARQLDEALEMIEREADDRMAPMLLMGRDFDAWEERGR
CCCHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHHHCHHHCCHHEECCCCHHHHHCCC
LLVQMPERLLDLPPPSLFGGYQFANAGLAVAAALTFDHDLPDEVLGDGVASAVWPARMQR
EEEECHHHHHCCCCCHHHCCCHHHCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHH
LTAGPLFELARARGADIWLDGGHNPHAGRALAEAASRIVDRDPRPLTLVAGMFARKDAEG
HCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHH
FFRPFAEMKPRVIATTFDSPQAASAEDIAVAAVKAGLPAEMSPNVEDAVRRALSVDGPAP
HHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCC
HVLVCGGLHFAGEVLAMSPETWPA
EEEEECCHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]