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The map label for this gene is ptrB [C]

Identifier: 197103591

GI number: 197103591

Start: 116574

End: 118655

Strand: Reverse

Name: ptrB [C]

Synonym: PHZ_c0125

Alternate gene names: 197103591

Gene position: 118655-116574 (Counterclockwise)

Preceding gene: 197103596

Following gene: 197103588

Centisome position: 2.97

GC content: 71.23

Gene sequence:

>2082_bases
ATGAAACGCCTCGCCTGCGCCCTCGCCGCCCTGTCGATTTCCGCGCCCGCCGCCCTCGCTCCCCCCGCCCTGGCCCAAGA
CGATCCGCACCTGTGGCTCGAGGAGGTGGAAAGCCCCAGGGCGCTCGCCTGGGCCGAGGCCGAGAACGCCAAGACCGCAG
CTGTCCTGGAGACGGACCCCCGTTACCAGGCGATGCTGGCCGAGGCGCGCGCCATCTTCACCGCCAAGGACCGCATCCCC
ACGCCGGGCTTCCGGGCCGGCGGGATCGACAACCTGTGGCAGGACGCCGAGCACCCGCACGGCCTGTGGCGGCATACGAC
CCGGGCCTCGTACGGCGCCGCCCAGCCTGCCTGGGAGACGCTGATCGACATGGACGCCCTGGCCAAGACCGAGGGCCGGC
GCTGGTTCTTCAAGGGCGCGGTGTGCCTGAAGCCCGAGGAGACGACCTGCCTCGTCCGCCTCTCCGACGGCGGCGGCGAC
GCGGTCGAACTGCGCGAGTTCGACACCCGCACCCGACGGTTCGTGGAGGGCGGCTTCCGCTTCGCCGAGGGCAAGCAGTA
CGTCCAGTGGATCGACCGCCACACGCTGGTCGTCGGCCGCGAATGGACCCCCGGCGAGGTCACCCAGTCCGGCTATCCCT
ATGTGCTGAAGACGGTCGCCCGCGACGGGACGGCGCGCGAGGTCTTCCGCGGCCAGAAGGCTGACGTCTGGGCCCAGCCC
GCCGTGCTCTACGGCGAGGGCGGCCGGGCCGACGGCCTGGTGGTCCAGCGGGGCCTGTCGTTCTTCGAGTCCGAGTTCAG
CCTCGTCACTGATCGGGGCCTCGTCCGCATCCAGCTGCCGAAGAAGGCGCAGGAGGAGGCCTATGTGGACGGCCAGGTGG
TGTTCAGCCTGCAGGAGGCCTGGGGCGGCTTCCAGCCCGGCGCCCTCGTCGCCTACGACCTGGCGGCCCTGAAGGCCGAC
CCCGCCGGCGCGCGCCCGACGCTCGTCTTCCAGCCCGGCCCGCGCCAGGCGGTGCAGCAGGTCTCGGCCACCGACGGGAA
GCTGGTGGTGACGCTGCTCGAGGACGTGAAGGGCGCGGTCGACGCCTACGACTTCGCGGACGGCCGCTGGACCGCCCGGC
GCCTGCCGCTGCCGAAGGACGCCAACATCACCCTGGTGGACACCTCCGGGAAGGACGACGCCCTGTTCGTCACGGTCGAG
GGCTTCCTGACCCCCACCAGCCTCTGGCTCGCCGACGCCGCGAGCGGGACGGCGCAGCAGGTGAAAACCCTGCCGCCCCG
TTTCGACGCCTCGACCCACGTCGTGGAGCAGTTCTGGGCGACCTCCTCCGACGGCGCGAAGATCCCCTACTTCATCGTGC
GCCCGAAGGCGGCGAAGCTGGACGGCTCCCTGCCCACCCTGATGTACGGCTACGGCGGCTTCGAGGTCGCCAAGCCGCCC
ATCTACATGCCCGAGGCCGGCAAGCTGTGGATGGAGCGGGGCGGCGCCTACGTCATCGCCAACATCCGCGGCGGCGGCGA
GTTCGGACCCGCCTGGCACCAGGCGGTGCTGCGCGAGAAGCGCCAGCTCGCCTTCGACGACTTCGCCGCCGTGGCCCGCG
ACCTGTTCGCACGCAAGATCACCTCGGCGCGCAGGCTCGGCATCTACGGCCGCTCCAACGGCGGGGTGCTGACCACCGTG
TCCATGACCCAGCATCCCGAGCTGTGGAACGCCGTCGTCGTCGAGAGCCCGCTGGTCGACATGCTGCGCTACCACAAGCT
CTCGGCCGGGGCGTCCTGGGTCGGCGAGTACGGCGATCCCGACGTGGCCGAGGACCGCGCGTTCATCGCGAAATACTCCG
GCTACCAGAACCTGAAGCCGGGCGTGCGCTACCCGCTGGCCTACATCACCACGAACACCCGCGACGACCGGGTCCATCCG
GGCCACGCGCGCAAGTACGCCGCGCGCCTCGCCGAGCTGGGGGTCCCCTACCTCTACTACGAGCAGACCTTCGGCGGGCA
CGCCAACGACGCCGATCCCGAGCTGAACGCCCGGCGTTGGGCGCGGCACTACGTCTACCTGGCCCAGCGGCTGATGGACT
GA

Upstream 100 bases:

>100_bases
TTAATCAAGCGTTCCAATCAGGCCGCCTTTTTCGAATCAGGCGTACGGCGGCCGGCGAGGAGGGGCAGACAAGCCCGTGG
CGGCCCGGCTAGATGAGGCC

Downstream 100 bases:

>100_bases
AGGGGGGGGGCCTCAGGCCTCCTTCAGCATGTCGGCCAGCAGCCGGCCCTCCGCCCGGCGGTTCGAGCCGGCGCGCCAGG
CGACGACGATCTCGCGCTCG

Product: serine protease of the peptidase family S9A

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MKRLACALAALSISAPAALAPPALAQDDPHLWLEEVESPRALAWAEAENAKTAAVLETDPRYQAMLAEARAIFTAKDRIP
TPGFRAGGIDNLWQDAEHPHGLWRHTTRASYGAAQPAWETLIDMDALAKTEGRRWFFKGAVCLKPEETTCLVRLSDGGGD
AVELREFDTRTRRFVEGGFRFAEGKQYVQWIDRHTLVVGREWTPGEVTQSGYPYVLKTVARDGTAREVFRGQKADVWAQP
AVLYGEGGRADGLVVQRGLSFFESEFSLVTDRGLVRIQLPKKAQEEAYVDGQVVFSLQEAWGGFQPGALVAYDLAALKAD
PAGARPTLVFQPGPRQAVQQVSATDGKLVVTLLEDVKGAVDAYDFADGRWTARRLPLPKDANITLVDTSGKDDALFVTVE
GFLTPTSLWLADAASGTAQQVKTLPPRFDASTHVVEQFWATSSDGAKIPYFIVRPKAAKLDGSLPTLMYGYGGFEVAKPP
IYMPEAGKLWMERGGAYVIANIRGGGEFGPAWHQAVLREKRQLAFDDFAAVARDLFARKITSARRLGIYGRSNGGVLTTV
SMTQHPELWNAVVVESPLVDMLRYHKLSAGASWVGEYGDPDVAEDRAFIAKYSGYQNLKPGVRYPLAYITTNTRDDRVHP
GHARKYAARLAELGVPYLYYEQTFGGHANDADPELNARRWARHYVYLAQRLMD

Sequences:

>Translated_693_residues
MKRLACALAALSISAPAALAPPALAQDDPHLWLEEVESPRALAWAEAENAKTAAVLETDPRYQAMLAEARAIFTAKDRIP
TPGFRAGGIDNLWQDAEHPHGLWRHTTRASYGAAQPAWETLIDMDALAKTEGRRWFFKGAVCLKPEETTCLVRLSDGGGD
AVELREFDTRTRRFVEGGFRFAEGKQYVQWIDRHTLVVGREWTPGEVTQSGYPYVLKTVARDGTAREVFRGQKADVWAQP
AVLYGEGGRADGLVVQRGLSFFESEFSLVTDRGLVRIQLPKKAQEEAYVDGQVVFSLQEAWGGFQPGALVAYDLAALKAD
PAGARPTLVFQPGPRQAVQQVSATDGKLVVTLLEDVKGAVDAYDFADGRWTARRLPLPKDANITLVDTSGKDDALFVTVE
GFLTPTSLWLADAASGTAQQVKTLPPRFDASTHVVEQFWATSSDGAKIPYFIVRPKAAKLDGSLPTLMYGYGGFEVAKPP
IYMPEAGKLWMERGGAYVIANIRGGGEFGPAWHQAVLREKRQLAFDDFAAVARDLFARKITSARRLGIYGRSNGGVLTTV
SMTQHPELWNAVVVESPLVDMLRYHKLSAGASWVGEYGDPDVAEDRAFIAKYSGYQNLKPGVRYPLAYITTNTRDDRVHP
GHARKYAARLAELGVPYLYYEQTFGGHANDADPELNARRWARHYVYLAQRLMD
>Mature_693_residues
MKRLACALAALSISAPAALAPPALAQDDPHLWLEEVESPRALAWAEAENAKTAAVLETDPRYQAMLAEARAIFTAKDRIP
TPGFRAGGIDNLWQDAEHPHGLWRHTTRASYGAAQPAWETLIDMDALAKTEGRRWFFKGAVCLKPEETTCLVRLSDGGGD
AVELREFDTRTRRFVEGGFRFAEGKQYVQWIDRHTLVVGREWTPGEVTQSGYPYVLKTVARDGTAREVFRGQKADVWAQP
AVLYGEGGRADGLVVQRGLSFFESEFSLVTDRGLVRIQLPKKAQEEAYVDGQVVFSLQEAWGGFQPGALVAYDLAALKAD
PAGARPTLVFQPGPRQAVQQVSATDGKLVVTLLEDVKGAVDAYDFADGRWTARRLPLPKDANITLVDTSGKDDALFVTVE
GFLTPTSLWLADAASGTAQQVKTLPPRFDASTHVVEQFWATSSDGAKIPYFIVRPKAAKLDGSLPTLMYGYGGFEVAKPP
IYMPEAGKLWMERGGAYVIANIRGGGEFGPAWHQAVLREKRQLAFDDFAAVARDLFARKITSARRLGIYGRSNGGVLTTV
SMTQHPELWNAVVVESPLVDMLRYHKLSAGASWVGEYGDPDVAEDRAFIAKYSGYQNLKPGVRYPLAYITTNTRDDRVHP
GHARKYAARLAELGVPYLYYEQTFGGHANDADPELNARRWARHYVYLAQRLMD

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=343, Percent_Identity=34.6938775510204, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI284172438, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI284172431, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI284172420, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI284172413, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI70778815, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI108860686, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI108860692, Length=213, Percent_Identity=27.2300469483568, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788150, Length=254, Percent_Identity=28.740157480315, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24583414, Length=321, Percent_Identity=34.2679127725857, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI221510989, Length=347, Percent_Identity=32.5648414985591, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 76412; Mature: 76412

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLACALAALSISAPAALAPPALAQDDPHLWLEEVESPRALAWAEAENAKTAAVLETDP
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCEEEEEECCC
RYQAMLAEARAIFTAKDRIPTPGFRAGGIDNLWQDAEHPHGLWRHTTRASYGAAQPAWET
HHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCCCCCHHHHH
LIDMDALAKTEGRRWFFKGAVCLKPEETTCLVRLSDGGGDAVELREFDTRTRRFVEGGFR
HHHHHHHHHCCCCEEEEECEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCCE
FAEGKQYVQWIDRHTLVVGREWTPGEVTQSGYPYVLKTVARDGTAREVFRGQKADVWAQP
ECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCC
AVLYGEGGRADGLVVQRGLSFFESEFSLVTDRGLVRIQLPKKAQEEAYVDGQVVFSLQEA
EEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCHHHCCCCCEEEEEHHH
WGGFQPGALVAYDLAALKADPAGARPTLVFQPGPRQAVQQVSATDGKLVVTLLEDVKGAV
CCCCCCCCEEEEHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHH
DAYDFADGRWTARRLPLPKDANITLVDTSGKDDALFVTVEGFLTPTSLWLADAASGTAQQ
HHCCCCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHCCCCCHHH
VKTLPPRFDASTHVVEQFWATSSDGAKIPYFIVRPKAAKLDGSLPTLMYGYGGFEVAKPP
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCHHCCCCCCCEEEEECCCCEECCCC
IYMPEAGKLWMERGGAYVIANIRGGGEFGPAWHQAVLREKRQLAFDDFAAVARDLFARKI
EECCCCCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSARRLGIYGRSNGGVLTTVSMTQHPELWNAVVVESPLVDMLRYHKLSAGASWVGEYGDP
HHHHHEEEEECCCCCEEEEEECCCCCCHHCEEEECCHHHHHHHHHHHCCCCHHCCCCCCC
DVAEDRAFIAKYSGYQNLKPGVRYPLAYITTNTRDDRVHPGHARKYAARLAELGVPYLYY
CCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
EQTFGGHANDADPELNARRWARHYVYLAQRLMD
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLACALAALSISAPAALAPPALAQDDPHLWLEEVESPRALAWAEAENAKTAAVLETDP
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCEEEEEECCC
RYQAMLAEARAIFTAKDRIPTPGFRAGGIDNLWQDAEHPHGLWRHTTRASYGAAQPAWET
HHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCCCCCHHHHH
LIDMDALAKTEGRRWFFKGAVCLKPEETTCLVRLSDGGGDAVELREFDTRTRRFVEGGFR
HHHHHHHHHCCCCEEEEECEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCCE
FAEGKQYVQWIDRHTLVVGREWTPGEVTQSGYPYVLKTVARDGTAREVFRGQKADVWAQP
ECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCC
AVLYGEGGRADGLVVQRGLSFFESEFSLVTDRGLVRIQLPKKAQEEAYVDGQVVFSLQEA
EEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCHHHCCCCCEEEEEHHH
WGGFQPGALVAYDLAALKADPAGARPTLVFQPGPRQAVQQVSATDGKLVVTLLEDVKGAV
CCCCCCCCEEEEHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHH
DAYDFADGRWTARRLPLPKDANITLVDTSGKDDALFVTVEGFLTPTSLWLADAASGTAQQ
HHCCCCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHCCCCCHHH
VKTLPPRFDASTHVVEQFWATSSDGAKIPYFIVRPKAAKLDGSLPTLMYGYGGFEVAKPP
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCHHCCCCCCCEEEEECCCCEECCCC
IYMPEAGKLWMERGGAYVIANIRGGGEFGPAWHQAVLREKRQLAFDDFAAVARDLFARKI
EECCCCCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSARRLGIYGRSNGGVLTTVSMTQHPELWNAVVVESPLVDMLRYHKLSAGASWVGEYGDP
HHHHHEEEEECCCCCEEEEEECCCCCCHHCEEEECCHHHHHHHHHHHCCCCHHCCCCCCC
DVAEDRAFIAKYSGYQNLKPGVRYPLAYITTNTRDDRVHPGHARKYAARLAELGVPYLYY
CCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
EQTFGGHANDADPELNARRWARHYVYLAQRLMD
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]