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The map label for this gene is nth [H]

Identifier: 197103504

GI number: 197103504

Start: 32311

End: 32985

Strand: Direct

Name: nth [H]

Synonym: PHZ_c0038

Alternate gene names: 197103504

Gene position: 32311-32985 (Clockwise)

Preceding gene: 197103502

Following gene: 197103505

Centisome position: 0.81

GC content: 69.19

Gene sequence:

>675_bases
ATGGCCAAGGCCCGCAAGACAACCCCGCTGAAGCCCGCCGAACGGGCCCGGATCGCCGAACTGTTCTCACGTTTCGAGAG
CCTGGAGGGCGATCCCCGCACCGAGCTGGACTACCAGGACCCCTATACCCTGGTCGTGGCCGTGGCCCTCTCGGCCCAGG
CCACCGACGTCTCGGTCAACAAGGCCACCGAGAAGCTGTTCGCCGTCGCCGACACGCCGCAGAAGATGCTGGCCCTGGGC
GAGGAGGGGCTGAAGCCCTTCATCTCCTCGATCGGCCTCTTCAACACCAAGGCCAAGAACGTCATCCGGATGGCGCAGAT
CCTCGTCGACCAGTACGGCGGCGAGGTCCCGCTCGAGCGCGAGAAGCTGCAGGCCCTGCCGGGCGTGGGGCGCAAGACCG
CCTCGGTGGTGCTGAACGAGCTGCGGATCGAGCCGGCCATCGCCGTGGACACCCACGTCTTCCGCGTCTCGCACCGGCTG
GAGCTGTCCGGCGGCAAGACGCCGGACGCGGTGGAGGCCGACCTGATGGCGATCGTGCCCGAGCCCTACCTGACGCGGGC
TCACCACTGGCTGATCCTGCACGGCCGCTACACCTGCACGGCCCGGCGGCCGAAGTGCGAGGACTGCCCCGTCGCCGACC
TCTGCCCCAGCCGGCATCTGTTCGTCGGCCGCTAG

Upstream 100 bases:

>100_bases
AGCGAGCATGGCTGAGCCGGAAAATACCGCGTCCGCGGGCGGCGCTCCACCGTGAACTCGCGCTGGCGGAGCCGGGCGGC
GCAGGCCATCTTCCGCGCCC

Downstream 100 bases:

>100_bases
CCGCCCCTAGCTCGTAGGGCGATTTCCCTGTAATCGGCCCCGCACGTCCCCGGGGAAGACATCCAGCAATGCCAGAGCTC
TACGACGTCGCCGCCATCGG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MAKARKTTPLKPAERARIAELFSRFESLEGDPRTELDYQDPYTLVVAVALSAQATDVSVNKATEKLFAVADTPQKMLALG
EEGLKPFISSIGLFNTKAKNVIRMAQILVDQYGGEVPLEREKLQALPGVGRKTASVVLNELRIEPAIAVDTHVFRVSHRL
ELSGGKTPDAVEADLMAIVPEPYLTRAHHWLILHGRYTCTARRPKCEDCPVADLCPSRHLFVGR

Sequences:

>Translated_224_residues
MAKARKTTPLKPAERARIAELFSRFESLEGDPRTELDYQDPYTLVVAVALSAQATDVSVNKATEKLFAVADTPQKMLALG
EEGLKPFISSIGLFNTKAKNVIRMAQILVDQYGGEVPLEREKLQALPGVGRKTASVVLNELRIEPAIAVDTHVFRVSHRL
ELSGGKTPDAVEADLMAIVPEPYLTRAHHWLILHGRYTCTARRPKCEDCPVADLCPSRHLFVGR
>Mature_223_residues
AKARKTTPLKPAERARIAELFSRFESLEGDPRTELDYQDPYTLVVAVALSAQATDVSVNKATEKLFAVADTPQKMLALGE
EGLKPFISSIGLFNTKAKNVIRMAQILVDQYGGEVPLEREKLQALPGVGRKTASVVLNELRIEPAIAVDTHVFRVSHRLE
LSGGKTPDAVEADLMAIVPEPYLTRAHHWLILHGRYTCTARRPKCEDCPVADLCPSRHLFVGR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=30.7262569832402, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=53.4653465346535, Blast_Score=232, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324530, Length=189, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550361, Length=202, Percent_Identity=30.1980198019802, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24754; Mature: 24623

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKARKTTPLKPAERARIAELFSRFESLEGDPRTELDYQDPYTLVVAVALSAQATDVSVN
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHEEHHHCCCCCCCHH
KATEKLFAVADTPQKMLALGEEGLKPFISSIGLFNTKAKNVIRMAQILVDQYGGEVPLER
HHHHHHEEECCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
EKLQALPGVGRKTASVVLNELRIEPAIAVDTHVFRVSHRLELSGGKTPDAVEADLMAIVP
HHHHHCCCCCHHHHHHHHHHHHCCCCEEEHHHEEEEEEEEEECCCCCCCHHHHHHHEECC
EPYLTRAHHWLILHGRYTCTARRPKCEDCPVADLCPSRHLFVGR
CHHHHCCEEEEEEECCEEEECCCCCCCCCCHHHCCCCCCEECCC
>Mature Secondary Structure 
AKARKTTPLKPAERARIAELFSRFESLEGDPRTELDYQDPYTLVVAVALSAQATDVSVN
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHEEHHHCCCCCCCHH
KATEKLFAVADTPQKMLALGEEGLKPFISSIGLFNTKAKNVIRMAQILVDQYGGEVPLER
HHHHHHEEECCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
EKLQALPGVGRKTASVVLNELRIEPAIAVDTHVFRVSHRLELSGGKTPDAVEADLMAIVP
HHHHHCCCCCHHHHHHHHHHHHCCCCEEEHHHEEEEEEEEEECCCCCCCHHHHHHHEECC
EPYLTRAHHWLILHGRYTCTARRPKCEDCPVADLCPSRHLFVGR
CHHHHCCEEEEEEECCEEEECCCCCCCCCCHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA