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The map label for this gene is hslU

Identifier: 197103501

GI number: 197103501

Start: 29659

End: 30963

Strand: Direct

Name: hslU

Synonym: PHZ_c0035

Alternate gene names: 197103501

Gene position: 29659-30963 (Clockwise)

Preceding gene: 197103500

Following gene: 197103502

Centisome position: 0.74

GC content: 70.8

Gene sequence:

>1305_bases
ATGACCGAATTTTCCCCGCGCGAGATCGTCTCCGAACTCGACCGCTTCATCGTCGGCCATCCGGAAGCCAAGCGCGCCGT
GGCCGTGGCCCTGCGCAACCGCTGGCGCCGCCGCCGCGTGCCCGACGACCTGCGCGACGAGGTGACGCCCAAGAACATCC
TGATGATCGGCCCGACCGGCGTCGGCAAGACCGAGATCGCCCGCCGGCTGGCCAAGCTGGCCCAGGCGCCGTTCCTGAAG
GTCGAGGCCACGAAGTTCACCGAGGTGGGCTACGTCGGCCGCGACGTGGACCAGATCGTCCGCGACCTGGTCGAGAGCGC
CATCCAGATGGTGCGCGAGAAGCGCCGGGCCGCCGTGCGCGCCCGCGCCGAGGCCGCCGCCGAGGAGCGGATTCTGGACG
CCCTCACCGGCCCCGGCTCCACCGCCGCCCGCGAGGCCTTCCGCAGGAAGCTGCGGGCCGGCGAGCTGGACGACAAGGAG
GTGGAGCTGCAGCTCGCCGACACCTCCTCGCCCTTCGGCGCCATGGACATCCCCGGCCAGCCCGGCGCGTCCATGGGCGT
GCTGAACCTGGGCGACATGTTCGGCAAGGCCTTCGGCGGCCGCACCAAGACCCACAAGACCACCGTCTCGGGCGCCTGGG
TCCCGCTGATCGCCGAGGAGAGCGACAAGCTGGTCGACCAGGAGGCCCTGACCCAGGAGGCGCTGGAGCTGGCCGAGAAC
CAGGGCATCGTCTTCCTGGACGAGATCGACAAGGTCGCCTCCCGCCAGGACCGCGCCGGCGCCGACGTCTCCCGCGAGGG
GGTGCAGCGCGACCTGCTGCCGCTGATCGAGGGGACCACCGTCTCGACCAAGCACGGGCCGGTGAAGACCGACCACATCC
TGTTCATCGCCTCGGGGGCCTTCCACGTGGCCAAGCCCTCGGACCTGCTGCCCGAGCTGCAGGGCCGCCTGCCGATCCGG
GTGGAGCTGAAGGCGCTGTCCCGCCAGGACATGCGCCGCATCCTGACCGAACCCGAGGCCAACCTGATCCGCCAGCACCA
GGCCCTGCTGGCCACCGAAGGCGTGACGCTGACCTTCACCGAGGACGCCATCGACGCCCTGGCCGACGCCGCCGTTGCGG
TGAACGGCTCGGTGGAGAACATCGGCGCCCGGCGGCTGCAGACCATCCTGGAGAAGGTGCTGGAGGAGGTGAGCTTCACC
GCCGCCGACCGCGACGGCGAGACGATCACGGTGGACGCGGCCTATGTGAACGGCCGGATCGGCGACCTGGCCGGCAACGC
CGACCTGTCGAAGTTCATCCTCTAG

Upstream 100 bases:

>100_bases
GGCGGCTGCTGTTCGACGAGCTGCTGGATGCGCCGGCTCCGACACCCCCCTCCGCAGCGCCGTCGCAAGCGGCCGGATCG
TGACTATCTAACCAGGCGAC

Downstream 100 bases:

>100_bases
GCGATGTGCGGGATCTGCCTGTCGCGGCGCGGAGTGCTGGCGGGCCTGGGGGCCCTGCCGCTCGGCGCCGCGTCCCTGGC
GGCCTGCAGCGAGAACGCCG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTEFSPREIVSELDRFIVGHPEAKRAVAVALRNRWRRRRVPDDLRDEVTPKNILMIGPTGVGKTEIARRLAKLAQAPFLK
VEATKFTEVGYVGRDVDQIVRDLVESAIQMVREKRRAAVRARAEAAAEERILDALTGPGSTAAREAFRRKLRAGELDDKE
VELQLADTSSPFGAMDIPGQPGASMGVLNLGDMFGKAFGGRTKTHKTTVSGAWVPLIAEESDKLVDQEALTQEALELAEN
QGIVFLDEIDKVASRQDRAGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELKALSRQDMRRILTEPEANLIRQHQALLATEGVTLTFTEDAIDALADAAVAVNGSVENIGARRLQTILEKVLEEVSFT
AADRDGETITVDAAYVNGRIGDLAGNADLSKFIL

Sequences:

>Translated_434_residues
MTEFSPREIVSELDRFIVGHPEAKRAVAVALRNRWRRRRVPDDLRDEVTPKNILMIGPTGVGKTEIARRLAKLAQAPFLK
VEATKFTEVGYVGRDVDQIVRDLVESAIQMVREKRRAAVRARAEAAAEERILDALTGPGSTAAREAFRRKLRAGELDDKE
VELQLADTSSPFGAMDIPGQPGASMGVLNLGDMFGKAFGGRTKTHKTTVSGAWVPLIAEESDKLVDQEALTQEALELAEN
QGIVFLDEIDKVASRQDRAGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELKALSRQDMRRILTEPEANLIRQHQALLATEGVTLTFTEDAIDALADAAVAVNGSVENIGARRLQTILEKVLEEVSFT
AADRDGETITVDAAYVNGRIGDLAGNADLSKFIL
>Mature_433_residues
TEFSPREIVSELDRFIVGHPEAKRAVAVALRNRWRRRRVPDDLRDEVTPKNILMIGPTGVGKTEIARRLAKLAQAPFLKV
EATKFTEVGYVGRDVDQIVRDLVESAIQMVREKRRAAVRARAEAAAEERILDALTGPGSTAAREAFRRKLRAGELDDKEV
ELQLADTSSPFGAMDIPGQPGASMGVLNLGDMFGKAFGGRTKTHKTTVSGAWVPLIAEESDKLVDQEALTQEALELAENQ
GIVFLDEIDKVASRQDRAGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIRV
ELKALSRQDMRRILTEPEANLIRQHQALLATEGVTLTFTEDAIDALADAAVAVNGSVENIGARRLQTILEKVLEEVSFTA
ADRDGETITVDAAYVNGRIGDLAGNADLSKFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=111, Percent_Identity=37.8378378378378, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=58.7443946188341, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1786642, Length=255, Percent_Identity=32.156862745098, Blast_Score=98, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648291, Length=308, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648289, Length=308, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_PHEZH (B4RBI9)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002128878.1
- GeneID:   6789522
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0035
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 47344; Mature: 47212

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 247-247 BINDING 312-312 BINDING 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFSPREIVSELDRFIVGHPEAKRAVAVALRNRWRRRRVPDDLRDEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLAQAPFLKVEATKFTEVGYVGRDVDQIVRDLVESAIQMVREKRRAAVR
CCHHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARAEAAAEERILDALTGPGSTAAREAFRRKLRAGELDDKEVELQLADTSSPFGAMDIPGQ
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCC
PGASMGVLNLGDMFGKAFGGRTKTHKTTVSGAWVPLIAEESDKLVDQEALTQEALELAEN
CCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
QGIVFLDEIDKVASRQDRAGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGA
CCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCC
FHVAKPSDLLPELQGRLPIRVELKALSRQDMRRILTEPEANLIRQHQALLATEGVTLTFT
EECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEC
EDAIDALADAAVAVNGSVENIGARRLQTILEKVLEEVSFTAADRDGETITVDAAYVNGRI
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCC
GDLAGNADLSKFIL
CCCCCCCCHHHHCC
>Mature Secondary Structure 
TEFSPREIVSELDRFIVGHPEAKRAVAVALRNRWRRRRVPDDLRDEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLAQAPFLKVEATKFTEVGYVGRDVDQIVRDLVESAIQMVREKRRAAVR
CCHHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARAEAAAEERILDALTGPGSTAAREAFRRKLRAGELDDKEVELQLADTSSPFGAMDIPGQ
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCC
PGASMGVLNLGDMFGKAFGGRTKTHKTTVSGAWVPLIAEESDKLVDQEALTQEALELAEN
CCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
QGIVFLDEIDKVASRQDRAGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGA
CCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCC
FHVAKPSDLLPELQGRLPIRVELKALSRQDMRRILTEPEANLIRQHQALLATEGVTLTFT
EECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEC
EDAIDALADAAVAVNGSVENIGARRLQTILEKVLEEVSFTAADRDGETITVDAAYVNGRI
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCC
GDLAGNADLSKFIL
CCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA