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The map label for this gene is fabI [H]

Identifier: 197103492

GI number: 197103492

Start: 22839

End: 23657

Strand: Reverse

Name: fabI [H]

Synonym: PHZ_c0026

Alternate gene names: 197103492

Gene position: 23657-22839 (Counterclockwise)

Preceding gene: 197103493

Following gene: 197103488

Centisome position: 0.59

GC content: 69.23

Gene sequence:

>819_bases
GTGGCCGACGACTATCAGATGCCGACCGGGACCCTGATGAAGGGCAAGAAGGGGATCGTCACGGGCGTGGCGAACCACCA
GTCCATCGCCTGGGGCATCGCCCGCCAGCTGGCCGCCCAGGGCGCCGAGATCGCCTTCCTGCACCTGCCGGGCATGGAGC
GGCGCGTGCAGCCGCTCGCCGACGAGATCGGCGTGAAGGTGCTGGCGCCCGTCGACGTCACCGACGACGGCGCCATGGAC
GCGGCCTTCCAGACGGTGAAAGACGCCTTCGGCCAGATCGACTTCTTCGTCCACGCCATCGCCTTCGCCAACAAGGACGA
GCTGAAGGGCAGCTTCGTGGACAACACCACCCGCGAAGGCTTCCTGCGGGCGATGAACATCTCGGCGTTCAGCTTCGTGG
ACTGCGCGCGGCGCGCGGCGGCGATGATGCCCGAGACCGGCGGCTCAATCATCACGCTCACCTACATGGGCTCGGAGCGG
GCGATCCCGAACTACAACACCATGGGCGTGGCCAAGGCCGCGCTCGAGGCCGCCGTGCGCTACGCCGCCCGCGACCTGGG
GCCCAAGGGCATCCGGGTCAACGCCGTCTCGCCGGGCGCCATGCGGACGCTGTCCCTGGCCGGCATCCAGGGCGGCCGCG
GCATGCTGGCCAAGGGCCGCTCGCTCAGCGCCATGAAGGAGGACACCTCGATGGAGGGCGTCGCCGGCTGCGCACTGTGG
CTCCTGTCCGACCTCGGCAAGTCCACCACCGGCGAATGCATCCACGTGGACGCCGGCTTCCACATCATGGGCTTCACGGA
AGACGAGGCCGAGGGCTGA

Upstream 100 bases:

>100_bases
GCGGCGCCCCGTCCGGCGACGGCCAGTGAAAGCTGAGTGATGACAGCGGCAGGTGGATCGGCCTAAACCGCGCCAATTCC
GACCACGAAGGGAGAGACAC

Downstream 100 bases:

>100_bases
GACCGCCTCAGGCGGCGTAGGCCTTCATGAAGCGGGCGGCGGCCTCGGTCGCCACCCGGTCCACCAGCGCCTCGGTCAGC
GGCCGTTCCACGCCCAGCAG

Product: enoyl-(acyl-carrier protein) reductase (NADH)

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MADDYQMPTGTLMKGKKGIVTGVANHQSIAWGIARQLAAQGAEIAFLHLPGMERRVQPLADEIGVKVLAPVDVTDDGAMD
AAFQTVKDAFGQIDFFVHAIAFANKDELKGSFVDNTTREGFLRAMNISAFSFVDCARRAAAMMPETGGSIITLTYMGSER
AIPNYNTMGVAKAALEAAVRYAARDLGPKGIRVNAVSPGAMRTLSLAGIQGGRGMLAKGRSLSAMKEDTSMEGVAGCALW
LLSDLGKSTTGECIHVDAGFHIMGFTEDEAEG

Sequences:

>Translated_272_residues
MADDYQMPTGTLMKGKKGIVTGVANHQSIAWGIARQLAAQGAEIAFLHLPGMERRVQPLADEIGVKVLAPVDVTDDGAMD
AAFQTVKDAFGQIDFFVHAIAFANKDELKGSFVDNTTREGFLRAMNISAFSFVDCARRAAAMMPETGGSIITLTYMGSER
AIPNYNTMGVAKAALEAAVRYAARDLGPKGIRVNAVSPGAMRTLSLAGIQGGRGMLAKGRSLSAMKEDTSMEGVAGCALW
LLSDLGKSTTGECIHVDAGFHIMGFTEDEAEG
>Mature_271_residues
ADDYQMPTGTLMKGKKGIVTGVANHQSIAWGIARQLAAQGAEIAFLHLPGMERRVQPLADEIGVKVLAPVDVTDDGAMDA
AFQTVKDAFGQIDFFVHAIAFANKDELKGSFVDNTTREGFLRAMNISAFSFVDCARRAAAMMPETGGSIITLTYMGSERA
IPNYNTMGVAKAALEAAVRYAARDLGPKGIRVNAVSPGAMRTLSLAGIQGGRGMLAKGRSLSAMKEDTSMEGVAGCALWL
LSDLGKSTTGECIHVDAGFHIMGFTEDEAEG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=42.8571428571429, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=27.0588235294118, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI2367365, Length=237, Percent_Identity=27.8481012658228, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=202, Percent_Identity=29.7029702970297, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=194, Percent_Identity=28.8659793814433, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=199, Percent_Identity=30.6532663316583, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=211, Percent_Identity=24.6445497630332, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21357041, Length=181, Percent_Identity=28.7292817679558, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571526, Length=206, Percent_Identity=29.126213592233, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17737361, Length=218, Percent_Identity=30.2752293577982, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28756; Mature: 28625

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDYQMPTGTLMKGKKGIVTGVANHQSIAWGIARQLAAQGAEIAFLHLPGMERRVQPLA
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
DEIGVKVLAPVDVTDDGAMDAAFQTVKDAFGQIDFFVHAIAFANKDELKGSFVDNTTREG
HHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHH
FLRAMNISAFSFVDCARRAAAMMPETGGSIITLTYMGSERAIPNYNTMGVAKAALEAAVR
HHEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
YAARDLGPKGIRVNAVSPGAMRTLSLAGIQGGRGMLAKGRSLSAMKEDTSMEGVAGCALW
HHHHHCCCCCEEEEEECCCHHHEEEEECCCCCCCCEECCCCCHHHHHCCCCHHHHHHHHH
LLSDLGKSTTGECIHVDAGFHIMGFTEDEAEG
HHHHHCCCCCCCEEEEECCEEEEEECCCCCCC
>Mature Secondary Structure 
ADDYQMPTGTLMKGKKGIVTGVANHQSIAWGIARQLAAQGAEIAFLHLPGMERRVQPLA
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
DEIGVKVLAPVDVTDDGAMDAAFQTVKDAFGQIDFFVHAIAFANKDELKGSFVDNTTREG
HHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHH
FLRAMNISAFSFVDCARRAAAMMPETGGSIITLTYMGSERAIPNYNTMGVAKAALEAAVR
HHEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
YAARDLGPKGIRVNAVSPGAMRTLSLAGIQGGRGMLAKGRSLSAMKEDTSMEGVAGCALW
HHHHHCCCCCEEEEEECCCHHHEEEEECCCCCCCCEECCCCCHHHHHCCCCHHHHHHHHH
LLSDLGKSTTGECIHVDAGFHIMGFTEDEAEG
HHHHHCCCCCCCEEEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]