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The map label for this gene is aroE

Identifier: 197103469

GI number: 197103469

Start: 1460

End: 2323

Strand: Direct

Name: aroE

Synonym: PHZ_c0003

Alternate gene names: 197103469

Gene position: 1460-2323 (Clockwise)

Preceding gene: 197103468

Following gene: 197103470

Centisome position: 0.04

GC content: 75.12

Gene sequence:

>864_bases
ATGAAGCCGCCGATCACGGGACGCACGGTCGTGGCCGGAGTCGCGGGCCAGCCCGTGGCCCATTCGCTCTCCCCGCTGAT
CCACAACGCCTGGCTGGCGGCGGCGGGGATCGACGGGGTCTATGTGGCCTTCGCCCCGGACGCCGACCGGTTCGAGGTGT
TCGTGGACGGCCTGCGCGGCGGGGCGATCCGCGGCCTGAACGTCACCATTCCCTTCAAGGAGGTGGCGCTCTCCATCGCC
GACGTCGTGAGCCCGCGGGCCGAGGCGGCGGCGGCGGCCAACCTGATCACCTTCGAGGCCGACGGCAGGGTCCTGGCCGA
CAACACCGACGGCGTGGGGCTGCTGGGCGCCCTTCAGGCCCAGGCCCCCGGCTGGCGGGCCGACGCCGGACCCGTGGCCG
TGCTCGGCGCGGGCGGGGCGGCGCGCGGGGCGGTGGCCGCCCTCGTCGCCGCCGGCGCGCCGCAGGTGCGGATCGTCAAC
CGCACCCTGGCCAAGGCCGAGGCCGTCGCCGGGGCGGTGGGCCGCATCGCCGTCGCCTGCCCGCTGGACGCGGCGGCCCA
AGCCTTCGAGGGCGCGACGGCGGTGATCAACGCCACCTCGGCCGGCCTCTCGGGTTCGCCCGGCCTGGACGTGCCGCTGG
AGGCGACCCCCGAGACGGCGGTCGTCATGGATATGGTCTACAAGCCGCTGGTCACCCCGTTCCTGCAGCGGGCCCAGGAC
CTTAGGCGGCGCACGGTGGACGGGCTGGAGATGCTGATCCGCCAGGCCGAACCCTCGTTCCAGGCCTTCTACGGCGCCAA
TCCGCCGGCGGGCGCGGACGTCCGCGCGCTGGCCCTGAAGACGCTGGAGGGCCGAGCCCCATGA

Upstream 100 bases:

>100_bases
CCCAGCTCTTCGAGAAGATCGACGGGGACTATTTCGCCATCCTGGGCCTGCCGCTGCTGGGCCTGCTCGACCTCCTCAGA
CGACATGGAGCCCTCGCCGC

Downstream 100 bases:

>100_bases
AGATCGTCGGCCTCACCGGCTCCATCGGCATGGGCAAGTCCACCACCGCGGCGATGTTCCGCGACGCCGGAATCCCCGTC
TACGACGCCGACGCGGCGGT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKPPITGRTVVAGVAGQPVAHSLSPLIHNAWLAAAGIDGVYVAFAPDADRFEVFVDGLRGGAIRGLNVTIPFKEVALSIA
DVVSPRAEAAAAANLITFEADGRVLADNTDGVGLLGALQAQAPGWRADAGPVAVLGAGGAARGAVAALVAAGAPQVRIVN
RTLAKAEAVAGAVGRIAVACPLDAAAQAFEGATAVINATSAGLSGSPGLDVPLEATPETAVVMDMVYKPLVTPFLQRAQD
LRRRTVDGLEMLIRQAEPSFQAFYGANPPAGADVRALALKTLEGRAP

Sequences:

>Translated_287_residues
MKPPITGRTVVAGVAGQPVAHSLSPLIHNAWLAAAGIDGVYVAFAPDADRFEVFVDGLRGGAIRGLNVTIPFKEVALSIA
DVVSPRAEAAAAANLITFEADGRVLADNTDGVGLLGALQAQAPGWRADAGPVAVLGAGGAARGAVAALVAAGAPQVRIVN
RTLAKAEAVAGAVGRIAVACPLDAAAQAFEGATAVINATSAGLSGSPGLDVPLEATPETAVVMDMVYKPLVTPFLQRAQD
LRRRTVDGLEMLIRQAEPSFQAFYGANPPAGADVRALALKTLEGRAP
>Mature_287_residues
MKPPITGRTVVAGVAGQPVAHSLSPLIHNAWLAAAGIDGVYVAFAPDADRFEVFVDGLRGGAIRGLNVTIPFKEVALSIA
DVVSPRAEAAAAANLITFEADGRVLADNTDGVGLLGALQAQAPGWRADAGPVAVLGAGGAARGAVAALVAAGAPQVRIVN
RTLAKAEAVAGAVGRIAVACPLDAAAQAFEGATAVINATSAGLSGSPGLDVPLEATPETAVVMDMVYKPLVTPFLQRAQD
LRRRTVDGLEMLIRQAEPSFQAFYGANPPAGADVRALALKTLEGRAP

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=265, Percent_Identity=36.2264150943396, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787983, Length=277, Percent_Identity=29.6028880866426, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320332, Length=276, Percent_Identity=27.536231884058, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_PHEZH (B4RBF7)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002128846.1
- GeneID:   6789489
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0003
- HOGENOM:   HBG553408
- OMA:   CEITITN
- ProtClustDB:   CLSK889644
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 28989; Mature: 28989

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: ACT_SITE 73-73

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPITGRTVVAGVAGQPVAHSLSPLIHNAWLAAAGIDGVYVAFAPDADRFEVFVDGLRG
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHEEECCCC
GAIRGLNVTIPFKEVALSIADVVSPRAEAAAAANLITFEADGRVLADNTDGVGLLGALQA
CEEECEEEEECHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCEEEECCCCCCEEEEEHHC
QAPGWRADAGPVAVLGAGGAARGAVAALVAAGAPQVRIVNRTLAKAEAVAGAVGRIAVAC
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHEEEEC
PLDAAAQAFEGATAVINATSAGLSGSPGLDVPLEATPETAVVMDMVYKPLVTPFLQRAQD
CCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRRRTVDGLEMLIRQAEPSFQAFYGANPPAGADVRALALKTLEGRAP
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPPITGRTVVAGVAGQPVAHSLSPLIHNAWLAAAGIDGVYVAFAPDADRFEVFVDGLRG
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHEEECCCC
GAIRGLNVTIPFKEVALSIADVVSPRAEAAAAANLITFEADGRVLADNTDGVGLLGALQA
CEEECEEEEECHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCEEEECCCCCCEEEEEHHC
QAPGWRADAGPVAVLGAGGAARGAVAALVAAGAPQVRIVNRTLAKAEAVAGAVGRIAVAC
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHEEEEC
PLDAAAQAFEGATAVINATSAGLSGSPGLDVPLEATPETAVVMDMVYKPLVTPFLQRAQD
CCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRRRTVDGLEMLIRQAEPSFQAFYGANPPAGADVRALALKTLEGRAP
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA