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The map label for this gene is recF [H]

Identifier: 195979034

GI number: 195979034

Start: 2005722

End: 2006831

Strand: Direct

Name: recF [H]

Synonym: Sez_1946

Alternate gene names: 195979034

Gene position: 2005722-2006831 (Clockwise)

Preceding gene: 195979033

Following gene: 195979040

Centisome position: 99.09

GC content: 40.27

Gene sequence:

>1110_bases
ATGTGGATCAAAGAACTAAACCTCACCCATTACCGTAATTATCAACAAGCCTCAGCAGCCTTTTCACCAGGCTTGAATGT
TTTCATTGGTGACAATGCGCAAGGAAAAACAAACTTTCTTGAAGCTATTTATTTTTTGTCAGTAACAAGAAGTCATCGCA
CCAAATCTGATAAGGACTTAATCTACTTTGACGAAAGGGATTGCTCTATATCTGGAACTCTTGAGCGCCTTAGTGGTCGT
GTTCAGCTAGAAATACTACTGTCTGATAAAGGACGGATCACCAAGATTAACACCCTAAAGCAGGCAAAGCTTTCTGATTA
TATCGGAGCAATGATGGTGGTCCTCTTTGCTCCTGAGGACCTACAGCTGGTCAAAGGCTCTCCTAGTCTTAGACGAAAAT
TTATGGATATTGACTTAGGTCAGATAAAGCCTGTTTACTTATCAGATTTATCACATTACAATCATGTCCTAAAGCAGCGA
AATGCCTATCTCAAAAGTGTTCATCAGCTGGATAGTGACTTTTTGTCTGTGTTAGATGAGCAGTTGGTTACCTATGGGAG
TCGTGTAATGGCGCATCGGTTGGCGTTTGTTCAATCACTAGCAAAAGAGGCAAACAAGCATCATCAAGCTATCTCTAATG
GCCTAGAGAAGCTGTCAATCTCCTATCAAGCCTCTGTAAGCTTTGAGCACCAGCAAGAGATTTATCAGCAATTCATGGAC
CAGCTCAAAACCACTCATCAAAGAGATTTCTTGAGAAAAAATACTGGTGTTGGTCCCCACCGCGATGATCTGATCTTTTA
TATTAATGGCATGAACGCTAATTTTGCTAGTCAAGGCCAGCATCGGAGCCTGATTTTATCACTGAAAATGGCAGAGGTTA
GTCTGATGAAGCAATTAACAGGTGACAACCCTATTCTTCTACTTGACGATGTTATGAGTGAGTTAGACAATATCAGGCAA
ACAAAACTTCTGGAGGCTGTCAAGAAAGAAAATGTTCAAACCTTTATCACAACAACTAGCTTAAAGCATTTATCTCAGCT
ACCCAAGGACATTAGTCTCTTTAAGGTTAATAAAGGGACGATTGCACTAGATTCTGTCATGATTGATTAA

Upstream 100 bases:

>100_bases
AAGCAAGCTGCTGCTACCAAAAGCAAGCGTGGTCAAGCTATCTCAGCTGTAAAAGCTAAGGCTAATCAACAAAAGCCTAT
TCGCTTCCCAGGATTGTAGC

Downstream 100 bases:

>100_bases
CTGAGTAAACCAAAAAAGGGCACCTTAACAGTGGCCCTTTTTATGGTTAAATCAGTTGATACTCTATGACTTGACGATAC
CTAAGAGGATAGCGCCAACA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MWIKELNLTHYRNYQQASAAFSPGLNVFIGDNAQGKTNFLEAIYFLSVTRSHRTKSDKDLIYFDERDCSISGTLERLSGR
VQLEILLSDKGRITKINTLKQAKLSDYIGAMMVVLFAPEDLQLVKGSPSLRRKFMDIDLGQIKPVYLSDLSHYNHVLKQR
NAYLKSVHQLDSDFLSVLDEQLVTYGSRVMAHRLAFVQSLAKEANKHHQAISNGLEKLSISYQASVSFEHQQEIYQQFMD
QLKTTHQRDFLRKNTGVGPHRDDLIFYINGMNANFASQGQHRSLILSLKMAEVSLMKQLTGDNPILLLDDVMSELDNIRQ
TKLLEAVKKENVQTFITTTSLKHLSQLPKDISLFKVNKGTIALDSVMID

Sequences:

>Translated_369_residues
MWIKELNLTHYRNYQQASAAFSPGLNVFIGDNAQGKTNFLEAIYFLSVTRSHRTKSDKDLIYFDERDCSISGTLERLSGR
VQLEILLSDKGRITKINTLKQAKLSDYIGAMMVVLFAPEDLQLVKGSPSLRRKFMDIDLGQIKPVYLSDLSHYNHVLKQR
NAYLKSVHQLDSDFLSVLDEQLVTYGSRVMAHRLAFVQSLAKEANKHHQAISNGLEKLSISYQASVSFEHQQEIYQQFMD
QLKTTHQRDFLRKNTGVGPHRDDLIFYINGMNANFASQGQHRSLILSLKMAEVSLMKQLTGDNPILLLDDVMSELDNIRQ
TKLLEAVKKENVQTFITTTSLKHLSQLPKDISLFKVNKGTIALDSVMID
>Mature_369_residues
MWIKELNLTHYRNYQQASAAFSPGLNVFIGDNAQGKTNFLEAIYFLSVTRSHRTKSDKDLIYFDERDCSISGTLERLSGR
VQLEILLSDKGRITKINTLKQAKLSDYIGAMMVVLFAPEDLQLVKGSPSLRRKFMDIDLGQIKPVYLSDLSHYNHVLKQR
NAYLKSVHQLDSDFLSVLDEQLVTYGSRVMAHRLAFVQSLAKEANKHHQAISNGLEKLSISYQASVSFEHQQEIYQQFMD
QLKTTHQRDFLRKNTGVGPHRDDLIFYINGMNANFASQGQHRSLILSLKMAEVSLMKQLTGDNPILLLDDVMSELDNIRQ
TKLLEAVKKENVQTFITTTSLKHLSQLPKDISLFKVNKGTIALDSVMID

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=364, Percent_Identity=26.0989010989011, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42028; Mature: 42028

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIKELNLTHYRNYQQASAAFSPGLNVFIGDNAQGKTNFLEAIYFLSVTRSHRTKSDKDL
CCCCCCCHHHHCCHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
IYFDERDCSISGTLERLSGRVQLEILLSDKGRITKINTLKQAKLSDYIGAMMVVLFAPED
EEECCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCC
LQLVKGSPSLRRKFMDIDLGQIKPVYLSDLSHYNHVLKQRNAYLKSVHQLDSDFLSVLDE
CEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVTYGSRVMAHRLAFVQSLAKEANKHHQAISNGLEKLSISYQASVSFEHQQEIYQQFMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHH
QLKTTHQRDFLRKNTGVGPHRDDLIFYINGMNANFASQGQHRSLILSLKMAEVSLMKQLT
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHC
GDNPILLLDDVMSELDNIRQTKLLEAVKKENVQTFITTTSLKHLSQLPKDISLFKVNKGT
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCEEEEECCCC
IALDSVMID
EEEEEEECC
>Mature Secondary Structure
MWIKELNLTHYRNYQQASAAFSPGLNVFIGDNAQGKTNFLEAIYFLSVTRSHRTKSDKDL
CCCCCCCHHHHCCHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
IYFDERDCSISGTLERLSGRVQLEILLSDKGRITKINTLKQAKLSDYIGAMMVVLFAPED
EEECCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCC
LQLVKGSPSLRRKFMDIDLGQIKPVYLSDLSHYNHVLKQRNAYLKSVHQLDSDFLSVLDE
CEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVTYGSRVMAHRLAFVQSLAKEANKHHQAISNGLEKLSISYQASVSFEHQQEIYQQFMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHH
QLKTTHQRDFLRKNTGVGPHRDDLIFYINGMNANFASQGQHRSLILSLKMAEVSLMKQLT
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHC
GDNPILLLDDVMSELDNIRQTKLLEAVKKENVQTFITTTSLKHLSQLPKDISLFKVNKGT
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCEEEEECCCC
IALDSVMID
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA