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The map label for this gene is gph [C]

Identifier: 195979023

GI number: 195979023

Start: 1996928

End: 1997563

Strand: Direct

Name: gph [C]

Synonym: Sez_1935

Alternate gene names: 195979023

Gene position: 1996928-1997563 (Clockwise)

Preceding gene: 195979022

Following gene: 195979033

Centisome position: 98.65

GC content: 37.26

Gene sequence:

>636_bases
ATGAAAGCAATCCTATTTGACTTAGATGGTACTTTGGTGGACTCTAGTATAGGAATACTAAACGCCTTTGAATACACCTT
TCAAAAAATGAAAATCCCTTGTCCTGATCATAGCATATTATCGTCTTATATCGGACCACCCTTAGAAGCAACCTTTTCAG
CATTTTTCAAGGATCAGCATGATGTCGAGCTTGCTATCACACACTTTAGAGATTTTTATAAGGAATCAGGTATTTACCAA
GCAAGGCTTTATCCTGAAATCACAGAGCTATTAGATACTCTCTCACAAAAAAACCTTGACCTTTATGTGACAACTAGTAA
ATATGAGCCTATGGCAAGGCAAATGCTTCAGGATTTAACAATTAGCCATTATTTCAAGGCTATCCTAGGCTCTATTCCTA
ATCGTTGCCACAAGTTGGATATCATCAAGGCATGCTTACAAGATTTTGCCATTAATAAAAAAGAGGCTATAATTATCGGA
GATACCAAATTTGATATGATTGGTGGTAAACAAGCAGGTATTGCAAGACTTGGTGTTACTTGGGGATTTGGTTGTCAAGA
GGATTTACAAGCACATGGCGCACAATACATTGGAGAAACACCCCAAGAGGTGATTGGGATTATTCAATCATTGTAA

Upstream 100 bases:

>100_bases
AGCTGAGGGAGGATTAGCAGCAACCCCAACAGGTAGACGGTATAGTAAAGAGATTTTCGGAGACTAATATAATCACGTAA
AGATAGACTGAGGAGACAGT

Downstream 100 bases:

>100_bases
AGCGATACTAAAAACCTATCATCTCGTGTGATGATAGGTTTTTTAACGATGTTATCTGTTCTTAATAGCCCCAAGCTGAC
AAGCCCTGTGCACGATAGGC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKAILFDLDGTLVDSSIGILNAFEYTFQKMKIPCPDHSILSSYIGPPLEATFSAFFKDQHDVELAITHFRDFYKESGIYQ
ARLYPEITELLDTLSQKNLDLYVTTSKYEPMARQMLQDLTISHYFKAILGSIPNRCHKLDIIKACLQDFAINKKEAIIIG
DTKFDMIGGKQAGIARLGVTWGFGCQEDLQAHGAQYIGETPQEVIGIIQSL

Sequences:

>Translated_211_residues
MKAILFDLDGTLVDSSIGILNAFEYTFQKMKIPCPDHSILSSYIGPPLEATFSAFFKDQHDVELAITHFRDFYKESGIYQ
ARLYPEITELLDTLSQKNLDLYVTTSKYEPMARQMLQDLTISHYFKAILGSIPNRCHKLDIIKACLQDFAINKKEAIIIG
DTKFDMIGGKQAGIARLGVTWGFGCQEDLQAHGAQYIGETPQEVIGIIQSL
>Mature_211_residues
MKAILFDLDGTLVDSSIGILNAFEYTFQKMKIPCPDHSILSSYIGPPLEATFSAFFKDQHDVELAITHFRDFYKESGIYQ
ARLYPEITELLDTLSQKNLDLYVTTSKYEPMARQMLQDLTISHYFKAILGSIPNRCHKLDIIKACLQDFAINKKEAIIIG
DTKFDMIGGKQAGIARLGVTWGFGCQEDLQAHGAQYIGETPQEVIGIIQSL

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=216, Percent_Identity=23.6111111111111, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 23720; Mature: 23720

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAILFDLDGTLVDSSIGILNAFEYTFQKMKIPCPDHSILSSYIGPPLEATFSAFFKDQH
CCEEEEECCCCCHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCC
DVELAITHFRDFYKESGIYQARLYPEITELLDTLSQKNLDLYVTTSKYEPMARQMLQDLT
CHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
ISHYFKAILGSIPNRCHKLDIIKACLQDFAINKKEAIIIGDTKFDMIGGKQAGIARLGVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCEEECCE
WGFGCQEDLQAHGAQYIGETPQEVIGIIQSL
ECCCCHHHHHHCCHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKAILFDLDGTLVDSSIGILNAFEYTFQKMKIPCPDHSILSSYIGPPLEATFSAFFKDQH
CCEEEEECCCCCHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCC
DVELAITHFRDFYKESGIYQARLYPEITELLDTLSQKNLDLYVTTSKYEPMARQMLQDLT
CHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
ISHYFKAILGSIPNRCHKLDIIKACLQDFAINKKEAIIIGDTKFDMIGGKQAGIARLGVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCEEECCE
WGFGCQEDLQAHGAQYIGETPQEVIGIIQSL
ECCCCHHHHHHCCHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]