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The map label for this gene is dus [H]

Identifier: 195978929

GI number: 195978929

Start: 1902167

End: 1903108

Strand: Direct

Name: dus [H]

Synonym: Sez_1833

Alternate gene names: 195978929

Gene position: 1902167-1903108 (Clockwise)

Preceding gene: 195978928

Following gene: 195978930

Centisome position: 93.97

GC content: 47.56

Gene sequence:

>942_bases
GTGGAAATCCCTCACCGGACAGTGCTTGCACCTATGGCTGGTGTGACCAATTCGGCCTTTCGCACGATTGCCAAGGAATT
TGGGGCAGGTCTTGTTGTCATGGAGATGATTTCTGAAAAAGGACTTCTCTATAACAACGAAAAAACCCTGCATATGCTTC
ATATTGATGACAATGAACACCCCATATCAATCCAATTATTTGGTGGTAATGCTGAAGGGCTAAAGCGCGCAGCTGATTTT
ATCCAAAGCCATGCCAAGGCTGACATTGTTGACATCAATATGGGCTGTCCTGTTAACAAGGTGGTTAAAAATGAAGCAGG
TGCCAAGTGGCTTCGCGATCCCGATAAGATTTATCATATCATTCGGGAGGTAACCTCTGTCCTAGACATTCCGCTTACTG
TCAAAATGCGTACCGGCTGGGCAGACAGCTCACTAGCTGTTGAGAATGCCTTGGCTGCTGAAGCTGCTGGTGTGTCAGCT
CTTGCCATGCATGGCCGTACTCGTGAGCAGATGTATACTGGCACCTGTGACCATGAAACCCTTGCACGCGTCTCAAGAGC
TATTACCAGGATTCCATTTATTGGAAATGGAGATGTCAGAACCGTTCAGGACGCTGCATTTATGATTGAGGAGATCGGTG
TAGACGCGGTCATGATTGGCCGCGCAGCTATGAACAACCCTTATCTCTTCACCCAAATTAATCACTTCTTTGAGACAGGA
GAGGTTTTGCCAGAGCTGCCCTTTACTAAAAAGCTAGACATTGCAAGAGAGCACCTCAAGCGCCTCATTAGGCTCAAGGG
GGAGACTATTGCTGTTCGTGAATTTCGTGGGCTAGCTCCTCATTACCTGCGAGGTACAGCAGGCGCTGCCAAAATTCGCA
GTGCCGTTTCACGCGCTAATACCTTAGCTGAGGTTGAAACGATTTTTGCACAAATACACTAA

Upstream 100 bases:

>100_bases
ATTGTTTGCCAATTTTGTGGCACCAAGTACCAATTTGACCAAGCTGATTTGGAGGTGCTGATCAATGATAAGACTTAACT
CATCTTTTAAGATCGGCTCT

Downstream 100 bases:

>100_bases
GACTACATAGAAGGAGAAACATGTTGATTGTATTAGCTGGAACAATCGGCGCAGGCAAGAGTTCCCTTGCTGCTGCACTG
GGTGAGCATTTAGGAACAGA

Product: probable tRNA-dihydrouridine synthase Dus

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDDNEHPISIQLFGGNAEGLKRAADF
IQSHAKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHIIREVTSVLDIPLTVKMRTGWADSSLAVENALAAEAAGVSA
LAMHGRTREQMYTGTCDHETLARVSRAITRIPFIGNGDVRTVQDAAFMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETG
EVLPELPFTKKLDIAREHLKRLIRLKGETIAVREFRGLAPHYLRGTAGAAKIRSAVSRANTLAEVETIFAQIH

Sequences:

>Translated_313_residues
MEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDDNEHPISIQLFGGNAEGLKRAADF
IQSHAKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHIIREVTSVLDIPLTVKMRTGWADSSLAVENALAAEAAGVSA
LAMHGRTREQMYTGTCDHETLARVSRAITRIPFIGNGDVRTVQDAAFMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETG
EVLPELPFTKKLDIAREHLKRLIRLKGETIAVREFRGLAPHYLRGTAGAAKIRSAVSRANTLAEVETIFAQIH
>Mature_313_residues
MEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDDNEHPISIQLFGGNAEGLKRAADF
IQSHAKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHIIREVTSVLDIPLTVKMRTGWADSSLAVENALAAEAAGVSA
LAMHGRTREQMYTGTCDHETLARVSRAITRIPFIGNGDVRTVQDAAFMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETG
EVLPELPFTKKLDIAREHLKRLIRLKGETIAVREFRGLAPHYLRGTAGAAKIRSAVSRANTLAEVETIFAQIH

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=262, Percent_Identity=32.0610687022901, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI239788462, Length=261, Percent_Identity=32.183908045977, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=26.9911504424779, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI40807366, Length=258, Percent_Identity=27.906976744186, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=27.3092369477912, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1789660, Length=295, Percent_Identity=37.2881355932203, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1788462, Length=268, Percent_Identity=29.8507462686567, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693211, Length=242, Percent_Identity=22.3140495867769, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=29.6610169491525, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=251, Percent_Identity=27.4900398406374, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=26.8722466960352, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=243, Percent_Identity=24.2798353909465, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=28.3261802575107, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=286, Percent_Identity=25.1748251748252, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=23.1441048034935, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585320, Length=233, Percent_Identity=33.9055793991416, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=29.4372294372294, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=29.4372294372294, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921524, Length=253, Percent_Identity=28.4584980237154, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=29.7959183673469, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34505; Mature: 34505

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDDNEH
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCCEEEEEEECCCCC
PISIQLFGGNAEGLKRAADFIQSHAKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHI
EEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCHHCCCHHHHHHH
IREVTSVLDIPLTVKMRTGWADSSLAVENALAAEAAGVSALAMHGRTREQMYTGTCDHET
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHCCCCCCHHH
LARVSRAITRIPFIGNGDVRTVQDAAFMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHCCCCEEEHHHHHHHHHC
EVLPELPFTKKLDIAREHLKRLIRLKGETIAVREFRGLAPHYLRGTAGAAKIRSAVSRAN
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCHHHCCCCHHHHHHHHHHHHH
TLAEVETIFAQIH
HHHHHHHHHHHCC
>Mature Secondary Structure
MEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDDNEH
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCCEEEEEEECCCCC
PISIQLFGGNAEGLKRAADFIQSHAKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHI
EEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCHHCCCHHHHHHH
IREVTSVLDIPLTVKMRTGWADSSLAVENALAAEAAGVSALAMHGRTREQMYTGTCDHET
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHCCCCCCHHH
LARVSRAITRIPFIGNGDVRTVQDAAFMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHCCCCEEEHHHHHHHHHC
EVLPELPFTKKLDIAREHLKRLIRLKGETIAVREFRGLAPHYLRGTAGAAKIRSAVSRAN
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCHHHCCCCHHHHHHHHHHHHH
TLAEVETIFAQIH
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]