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The map label for this gene is def [H]

Identifier: 195978856

GI number: 195978856

Start: 1816708

End: 1817322

Strand: Direct

Name: def [H]

Synonym: Sez_1760

Alternate gene names: 195978856

Gene position: 1816708-1817322 (Clockwise)

Preceding gene: 195978844

Following gene: 195978871

Centisome position: 89.75

GC content: 43.09

Gene sequence:

>615_bases
ATGTCTGCACAAGATAAATTAATTAAAGCACAGCATCTCATTGATATGAATGATATTATCCGCGAAGGCAATCCTACGCT
TCGTGCTGTGGCTAAGGAGGTCGAGTTTCCTCTTAGTGATGATGACATCATTTTAGGTGAAAAAATGATGCAATTTTTAA
AGCATTCTCAAGACCCTGTCATGGGAGAAAAATTAGGGCTTCGAGCAGGAGTTGGTCTAGCAGCACCTCAGATTGATGTG
TCAAAGCGTATCATCGCTGTTCTGGTTCCAAATCCTGAAGACAGCGAGGGCAATCCACCTAAGGAGGCTTACAGCATGGA
GGAAGTGCTCTATAATCCTAAAATTGTGTCTCATTCTGTTCAAGACGCTGCTCTTGCTGACGGTGAGGGGTGTTTGTCTG
TTGATCGTGTGGTGGAAGGCTATGTCGTCCGTCATGCGCGTGTGACAGTGGAATATTACGATAAACACAACGAAAAACAC
CGCATCAAGCTAAAGGGCTACAATGCTATCGTAGTCCAGCATGAGATTGATCACATTAACGGTGTCTTATTTTATGACCG
TATTAATGCAAAAAACCCCTTTGAGGCTAAAGAGGGTATGCTAATTCTAGAGTAA

Upstream 100 bases:

>100_bases
AGTTAGCTAATATCGTCATCACTGGCCTGCCTTATCTTTTAAAAATAAGGAATTTGTGATATAGTAAAGCCATTATCAAA
TATAAAAAAAGGGAGGCAAA

Downstream 100 bases:

>100_bases
GCCACTATTATAGAACAAAGTCTTTCAAAAAGAGCTTCAAACCAGAATCGGTTTGAAGCTCTTTTGTGTGTCAAGCTGCT
TCTGAAGATGAGATACGGCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSAQDKLIKAQHLIDMNDIIREGNPTLRAVAKEVEFPLSDDDIILGEKMMQFLKHSQDPVMGEKLGLRAGVGLAAPQIDV
SKRIIAVLVPNPEDSEGNPPKEAYSMEEVLYNPKIVSHSVQDAALADGEGCLSVDRVVEGYVVRHARVTVEYYDKHNEKH
RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFEAKEGMLILE

Sequences:

>Translated_204_residues
MSAQDKLIKAQHLIDMNDIIREGNPTLRAVAKEVEFPLSDDDIILGEKMMQFLKHSQDPVMGEKLGLRAGVGLAAPQIDV
SKRIIAVLVPNPEDSEGNPPKEAYSMEEVLYNPKIVSHSVQDAALADGEGCLSVDRVVEGYVVRHARVTVEYYDKHNEKH
RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFEAKEGMLILE
>Mature_203_residues
SAQDKLIKAQHLIDMNDIIREGNPTLRAVAKEVEFPLSDDDIILGEKMMQFLKHSQDPVMGEKLGLRAGVGLAAPQIDVS
KRIIAVLVPNPEDSEGNPPKEAYSMEEVLYNPKIVSHSVQDAALADGEGCLSVDRVVEGYVVRHARVTVEYYDKHNEKHR
IKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFEAKEGMLILE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22837; Mature: 22706

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQDKLIKAQHLIDMNDIIREGNPTLRAVAKEVEFPLSDDDIILGEKMMQFLKHSQDPV
CCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCC
MGEKLGLRAGVGLAAPQIDVSKRIIAVLVPNPEDSEGNPPKEAYSMEEVLYNPKIVSHSV
CCHHHCHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHCCCC
QDAALADGEGCLSVDRVVEGYVVRHARVTVEYYDKHNEKHRIKLKGYNAIVVQHEIDHIN
CHHHHCCCCCCEEHHHHHHHHEEEEEEEEEEEECCCCCCEEEEEECCEEEEEEEECCCCC
GVLFYDRINAKNPFEAKEGMLILE
CEEEEEECCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
SAQDKLIKAQHLIDMNDIIREGNPTLRAVAKEVEFPLSDDDIILGEKMMQFLKHSQDPV
CCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCC
MGEKLGLRAGVGLAAPQIDVSKRIIAVLVPNPEDSEGNPPKEAYSMEEVLYNPKIVSHSV
CCHHHCHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHCCCC
QDAALADGEGCLSVDRVVEGYVVRHARVTVEYYDKHNEKHRIKLKGYNAIVVQHEIDHIN
CHHHHCCCCCCEEHHHHHHHHEEEEEEEEEEEECCCCCCEEEEEECCEEEEEEEECCCCC
GVLFYDRINAKNPFEAKEGMLILE
CEEEEEECCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA