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The map label for this gene is yclM [H]

Identifier: 195978827

GI number: 195978827

Start: 1779641

End: 1781005

Strand: Direct

Name: yclM [H]

Synonym: Sez_1731

Alternate gene names: 195978827

Gene position: 1779641-1781005 (Clockwise)

Preceding gene: 195978821

Following gene: 195978834

Centisome position: 87.92

GC content: 42.86

Gene sequence:

>1365_bases
ATGAAGGTAACAAAATTTGGCGGCAGCTCCTTAGCATCTGCCCAGCAGTTAGAAAAGGTCTTGGCTATTATCAAGGACGA
TCCTGAGCGTAAAATCATCGTTGTCTCCGCACCAGGCAAACGATCCCCTACAGACACAAAGGTGACTGATGCACTCATTG
CTTATTACAAAGCCTACGTCTCAGACCGTCATTATCACAAGCATTTGGAATGGATTATCAATCGTTACCAACAAATCTGT
AATGACCTAACTCTAGATAGCAAAATCATCACCCAAATCACAGCTTCTTTAGAGCAATTGGCACGGCTTCCAAAAGAACA
TAATCCCTATTTATACGATACCTTTCTTGCGGCCGGTGAGGACAATAATGCTAAGCTAGTAGCTGCCTACCTCAAGGCAA
ATGGCTTAACTGCAAGCTATCTCCACCCCAAAGATGCAGGTCTAATCGTAAGCAGTGAGCCACAGCATGCACGTCTTCTT
CCAATCAGCTACGATAAGCTAGAGGCCTTAAAGGATCATGATGATATTCTTGTTATTCCTGGATTCTTTGGTGTGACAAA
TGAAAACCAGATTTGTACCTTCTCACGTGGTGGCTCTGATATTACCGGCTCCATTATTGCAGCAGGAGTCACAGCTGACA
GCTATGAAAATTTTACAGATGTCAATGGCATCTTTGCTGCCCACCCTGGTGTTGTTAAGCAGCCACAGACGATCACTGAA
TTAACCTACAGAGAAATGCGCGAGTTAGCCTATGCTGGCTTTTCTGTCCTCCATGATGAAGCATTAATCCCTGCTTACCG
AGGGAAAATCCCAATCGTCATCAAAAACACTAACAATCCCAGCCACCCAGGTACAACAATTGTTCTTGAGCACCAGAGGA
AATCCAATCCCGTTGTCGGTATATCAGGTGATGATCACTTTGTCAGCATTAACTTATCTAAATACCTCATGAACAGAGAA
ATTGGATTTGGGCGCAAGCTCCTTCAGATATTAGAGGATCTCAATATCAGATGGGAGCACATGCCTACAGGTATTGATGA
CCTATCAATTGTACTTAGAGAAAGAGAATTGACCCCCATCAAAGAAGAAGAAATCCTTCGCAGACTTGTCCAGCAATTAG
AGGTTGATGAGGTATCCATCACCAGAAACCTTTCTATTATCATGATAGTCGGTGAAAACATGAAAAGTCATATTGGTGTT
ACATCTGCCGCAACAAAGGCTCTATCTGAACATCATATCAATATTGCTATGATTTCCCAAGGCTCAAGCGAGGTATCTGT
CATGTTTGTCATCAATAGCACCGATGAAAAAAAGGCCATTAGAGCCTTATATCAAACCTTTTTTAGTCCTGATCGGCCCC
TGTAA

Upstream 100 bases:

>100_bases
CAAGCTTGATTTGACGCCAACAGGCAAAAAAAGGCTACTGACAAGGACTAGCTTTTCTGCTATAATTTCTCTAATATTCA
ATAAGTAGACTGGAGAGCAA

Downstream 100 bases:

>100_bases
AGCTCCATATAACATCTCTTGTTAACATCAAGCTTCAAAAACAGCTCACAAGCTAGACAATGTCTCTAGTCTATGAGCTG
CTTTTATAATCTATAAGCTA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKVTKFGGSSLASAQQLEKVLAIIKDDPERKIIVVSAPGKRSPTDTKVTDALIAYYKAYVSDRHYHKHLEWIINRYQQIC
NDLTLDSKIITQITASLEQLARLPKEHNPYLYDTFLAAGEDNNAKLVAAYLKANGLTASYLHPKDAGLIVSSEPQHARLL
PISYDKLEALKDHDDILVIPGFFGVTNENQICTFSRGGSDITGSIIAAGVTADSYENFTDVNGIFAAHPGVVKQPQTITE
LTYREMRELAYAGFSVLHDEALIPAYRGKIPIVIKNTNNPSHPGTTIVLEHQRKSNPVVGISGDDHFVSINLSKYLMNRE
IGFGRKLLQILEDLNIRWEHMPTGIDDLSIVLRERELTPIKEEEILRRLVQQLEVDEVSITRNLSIIMIVGENMKSHIGV
TSAATKALSEHHINIAMISQGSSEVSVMFVINSTDEKKAIRALYQTFFSPDRPL

Sequences:

>Translated_454_residues
MKVTKFGGSSLASAQQLEKVLAIIKDDPERKIIVVSAPGKRSPTDTKVTDALIAYYKAYVSDRHYHKHLEWIINRYQQIC
NDLTLDSKIITQITASLEQLARLPKEHNPYLYDTFLAAGEDNNAKLVAAYLKANGLTASYLHPKDAGLIVSSEPQHARLL
PISYDKLEALKDHDDILVIPGFFGVTNENQICTFSRGGSDITGSIIAAGVTADSYENFTDVNGIFAAHPGVVKQPQTITE
LTYREMRELAYAGFSVLHDEALIPAYRGKIPIVIKNTNNPSHPGTTIVLEHQRKSNPVVGISGDDHFVSINLSKYLMNRE
IGFGRKLLQILEDLNIRWEHMPTGIDDLSIVLRERELTPIKEEEILRRLVQQLEVDEVSITRNLSIIMIVGENMKSHIGV
TSAATKALSEHHINIAMISQGSSEVSVMFVINSTDEKKAIRALYQTFFSPDRPL
>Mature_454_residues
MKVTKFGGSSLASAQQLEKVLAIIKDDPERKIIVVSAPGKRSPTDTKVTDALIAYYKAYVSDRHYHKHLEWIINRYQQIC
NDLTLDSKIITQITASLEQLARLPKEHNPYLYDTFLAAGEDNNAKLVAAYLKANGLTASYLHPKDAGLIVSSEPQHARLL
PISYDKLEALKDHDDILVIPGFFGVTNENQICTFSRGGSDITGSIIAAGVTADSYENFTDVNGIFAAHPGVVKQPQTITE
LTYREMRELAYAGFSVLHDEALIPAYRGKIPIVIKNTNNPSHPGTTIVLEHQRKSNPVVGISGDDHFVSINLSKYLMNRE
IGFGRKLLQILEDLNIRWEHMPTGIDDLSIVLRERELTPIKEEEILRRLVQQLEVDEVSITRNLSIIMIVGENMKSHIGV
TSAATKALSEHHINIAMISQGSSEVSVMFVINSTDEKKAIRALYQTFFSPDRPL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=480, Percent_Identity=25.625, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=23.2104121475054, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790376, Length=298, Percent_Identity=28.1879194630872, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=498, Percent_Identity=26.3052208835341, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50598; Mature: 50598

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTKFGGSSLASAQQLEKVLAIIKDDPERKIIVVSAPGKRSPTDTKVTDALIAYYKAYV
CEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
SDRHYHKHLEWIINRYQQICNDLTLDSKIITQITASLEQLARLPKEHNPYLYDTFLAAGE
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
DNNAKLVAAYLKANGLTASYLHPKDAGLIVSSEPQHARLLPISYDKLEALKDHDDILVIP
CCCCEEEEEEECCCCCEEEECCCCCCCEEEECCCCCCEEEECCHHHHHHHCCCCCEEEEC
GFFGVTNENQICTFSRGGSDITGSIIAAGVTADSYENFTDVNGIFAAHPGVVKQPQTITE
CEECCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
LTYREMRELAYAGFSVLHDEALIPAYRGKIPIVIKNTNNPSHPGTTIVLEHQRKSNPVVG
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEE
ISGDDHFVSINLSKYLMNREIGFGRKLLQILEDLNIRWEHMPTGIDDLSIVLRERELTPI
ECCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHEECCCCCCC
KEEEILRRLVQQLEVDEVSITRNLSIIMIVGENMKSHIGVTSAATKALSEHHINIAMISQ
CHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCHHHCCCHHHHHHHHHHCCEEEEEEEC
GSSEVSVMFVINSTDEKKAIRALYQTFFSPDRPL
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVTKFGGSSLASAQQLEKVLAIIKDDPERKIIVVSAPGKRSPTDTKVTDALIAYYKAYV
CEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
SDRHYHKHLEWIINRYQQICNDLTLDSKIITQITASLEQLARLPKEHNPYLYDTFLAAGE
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
DNNAKLVAAYLKANGLTASYLHPKDAGLIVSSEPQHARLLPISYDKLEALKDHDDILVIP
CCCCEEEEEEECCCCCEEEECCCCCCCEEEECCCCCCEEEECCHHHHHHHCCCCCEEEEC
GFFGVTNENQICTFSRGGSDITGSIIAAGVTADSYENFTDVNGIFAAHPGVVKQPQTITE
CEECCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
LTYREMRELAYAGFSVLHDEALIPAYRGKIPIVIKNTNNPSHPGTTIVLEHQRKSNPVVG
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEE
ISGDDHFVSINLSKYLMNREIGFGRKLLQILEDLNIRWEHMPTGIDDLSIVLRERELTPI
ECCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHEECCCCCCC
KEEEILRRLVQQLEVDEVSITRNLSIIMIVGENMKSHIGVTSAATKALSEHHINIAMISQ
CHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCHHHCCCHHHHHHHHHHCCEEEEEEEC
GSSEVSVMFVINSTDEKKAIRALYQTFFSPDRPL
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]