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The map label for this gene is murE [H]

Identifier: 195978717

GI number: 195978717

Start: 1663451

End: 1664896

Strand: Direct

Name: murE [H]

Synonym: Sez_1617

Alternate gene names: 195978717

Gene position: 1663451-1664896 (Clockwise)

Preceding gene: 195978694

Following gene: 195978718

Centisome position: 82.18

GC content: 45.02

Gene sequence:

>1446_bases
ATGATAACAATTGAGCACTTATTAACCATTTTAAAAAAGGACCATAATTTCCGAGAGGTTCTTGATCAAGACGGCTACCA
CTTTAACTATAGCGGTCTACAGTTTGACAGCCTTAGCTACGACAGCAGACAAGCAGATGAGCAGACGCTCTTTTTTGCTA
AGGGAGCTACTTTTAAGGCTGACTTTTTGAAACAGGCCATTGCCAAAGGACTAAAAATCTATGTTTCAGAGATTGACTAT
CAGGTGGGGATCCCTGTTATCCTTGTTAACAATATTAAGCACGCTATGAGTCTAGCTGCGATGGCCTTTTATGATTACCC
ACAGCATAAGCTCAAGCTATTGGCATTTACTGGAACAAAGGGCAAGACAACTGCTGCCTACTTTGCCTATCATATGCTAA
AGGAATCTCATAAGCCTGCCCTGCTCTCTACAATGAATACAACCTTAGATGGCAAAAGCTTTTTTAAATCTCAATTAACC
ACACCTGAAAGCCTTGATTTATTTGCAATGATGGCCCAGTGTGTGGCTAATGGCATGACGCATTTGGTGATGGAGGTCTC
TAGTCAAGCCTACTTGGTTGGGCGTGTCTACGGCTTAATCTTTGATGTCGGTGTCTTTCTTAATATTAGCCCCGATCACA
TCGGACCAATCGAGCACCCAAGCTTTGAGGATTATTTTTACCATAAGCGGCTCTTAATGGAAAATAGCCGAGCTGTTGTG
GTCAATAGCGGTATGGAGCATTTTAATATCCTAGCAGAGCAGGTGGCTGATAAGCCTCATGATTTTTATGGCAAGGGGGC
TTGTAACAGGCTGCTGCAGCGTCAGGCCTTTTCATTTCAAGCAGCCGGCAAGCTAGCTGGAGACTATGCGATTCAATTGA
TTGGCAGCTTCAACCAAGAAAATGCTATGGCTGCTGGACTGGCCTGTCTTCGACTGGGGGCAAGCCTAGAGGATATTCGA
TTAGGAATTGCTAAGACAAGGGTGCCAGGCCGCATGGAGGTCTTAACCATGACCAATCAGGCCAAGATTGTTGTGGATTA
TGCACATAACGGTGATAGCCTTGAAAAGCTCCTATCTGTTGTCGAAGAGCACCAGAAAGGCCAGATTTACTTGATTTTGG
GAGCACCGGGCAATAAAGGTGAGAGCCGTCGGGCTGATTTTGGGCAGGTCATCAACCAGCACCCCAATGTTCGTATTATT
CTGACTGCTGATGATCCTAATTTTGAGGACCCACAGCTGATTTCAGAGGAGATTGCTAGCTATATTCACCGCGAGGTTGA
TATTATCATTGACCGTAAGGAAGCAATCAAGGCTGGCATGGAGCGCTGTCAGAATGAGCAGGACGCCCTTATTATTGCTG
GTAAGGGAGCTGATGCTTATCAGATTGTCAAGGGACAAAAGACTGCCTATGCAGGTGATTTAGCGATTGCAAAGAGCTAC
CTATAG

Upstream 100 bases:

>100_bases
TTTTTCTGTCGACATATCTGTTCTTCCTAGTTTTTATAGCTTTATTATAAACTTTTGCTTGTGACTTGTAAAACAAGAAC
ATTAAGGCTAAAATAAGGCT

Downstream 100 bases:

>100_bases
ACTGGTCTAACAGGCTAAACAGTAATGATTCTTAAGATTACTGATAATGATTCTAAGCTGACTCCTTTTTTTAGCATAAG
CTCAGTAATTAGATTCTGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MITIEHLLTILKKDHNFREVLDQDGYHFNYSGLQFDSLSYDSRQADEQTLFFAKGATFKADFLKQAIAKGLKIYVSEIDY
QVGIPVILVNNIKHAMSLAAMAFYDYPQHKLKLLAFTGTKGKTTAAYFAYHMLKESHKPALLSTMNTTLDGKSFFKSQLT
TPESLDLFAMMAQCVANGMTHLVMEVSSQAYLVGRVYGLIFDVGVFLNISPDHIGPIEHPSFEDYFYHKRLLMENSRAVV
VNSGMEHFNILAEQVADKPHDFYGKGACNRLLQRQAFSFQAAGKLAGDYAIQLIGSFNQENAMAAGLACLRLGASLEDIR
LGIAKTRVPGRMEVLTMTNQAKIVVDYAHNGDSLEKLLSVVEEHQKGQIYLILGAPGNKGESRRADFGQVINQHPNVRII
LTADDPNFEDPQLISEEIASYIHREVDIIIDRKEAIKAGMERCQNEQDALIIAGKGADAYQIVKGQKTAYAGDLAIAKSY
L

Sequences:

>Translated_481_residues
MITIEHLLTILKKDHNFREVLDQDGYHFNYSGLQFDSLSYDSRQADEQTLFFAKGATFKADFLKQAIAKGLKIYVSEIDY
QVGIPVILVNNIKHAMSLAAMAFYDYPQHKLKLLAFTGTKGKTTAAYFAYHMLKESHKPALLSTMNTTLDGKSFFKSQLT
TPESLDLFAMMAQCVANGMTHLVMEVSSQAYLVGRVYGLIFDVGVFLNISPDHIGPIEHPSFEDYFYHKRLLMENSRAVV
VNSGMEHFNILAEQVADKPHDFYGKGACNRLLQRQAFSFQAAGKLAGDYAIQLIGSFNQENAMAAGLACLRLGASLEDIR
LGIAKTRVPGRMEVLTMTNQAKIVVDYAHNGDSLEKLLSVVEEHQKGQIYLILGAPGNKGESRRADFGQVINQHPNVRII
LTADDPNFEDPQLISEEIASYIHREVDIIIDRKEAIKAGMERCQNEQDALIIAGKGADAYQIVKGQKTAYAGDLAIAKSY
L
>Mature_481_residues
MITIEHLLTILKKDHNFREVLDQDGYHFNYSGLQFDSLSYDSRQADEQTLFFAKGATFKADFLKQAIAKGLKIYVSEIDY
QVGIPVILVNNIKHAMSLAAMAFYDYPQHKLKLLAFTGTKGKTTAAYFAYHMLKESHKPALLSTMNTTLDGKSFFKSQLT
TPESLDLFAMMAQCVANGMTHLVMEVSSQAYLVGRVYGLIFDVGVFLNISPDHIGPIEHPSFEDYFYHKRLLMENSRAVV
VNSGMEHFNILAEQVADKPHDFYGKGACNRLLQRQAFSFQAAGKLAGDYAIQLIGSFNQENAMAAGLACLRLGASLEDIR
LGIAKTRVPGRMEVLTMTNQAKIVVDYAHNGDSLEKLLSVVEEHQKGQIYLILGAPGNKGESRRADFGQVINQHPNVRII
LTADDPNFEDPQLISEEIASYIHREVDIIIDRKEAIKAGMERCQNEQDALIIAGKGADAYQIVKGQKTAYAGDLAIAKSY
L

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=497, Percent_Identity=29.1750503018109, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.7 [H]

Molecular weight: Translated: 53537; Mature: 53537

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIEHLLTILKKDHNFREVLDQDGYHFNYSGLQFDSLSYDSRQADEQTLFFAKGATFKA
CEEHHHHHHHHHCCCCHHHHHCCCCCEEECCCEEECCCCCCCCCCCCCEEEEECCCCCHH
DFLKQAIAKGLKIYVSEIDYQVGIPVILVNNIKHAMSLAAMAFYDYPQHKLKLLAFTGTK
HHHHHHHHHHHEEEHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GKTTAAYFAYHMLKESHKPALLSTMNTTLDGKSFFKSQLTTPESLDLFAMMAQCVANGMT
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
HLVMEVSSQAYLVGRVYGLIFDVGVFLNISPDHIGPIEHPSFEDYFYHKRLLMENSRAVV
HHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
VNSGMEHFNILAEQVADKPHDFYGKGACNRLLQRQAFSFQAAGKLAGDYAIQLIGSFNQE
EECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
NAMAAGLACLRLGASLEDIRLGIAKTRVPGRMEVLTMTNQAKIVVDYAHNGDSLEKLLSV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHH
VEEHQKGQIYLILGAPGNKGESRRADFGQVINQHPNVRIILTADDPNFEDPQLISEEIAS
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
YIHREVDIIIDRKEAIKAGMERCQNEQDALIIAGKGADAYQIVKGQKTAYAGDLAIAKSY
HHHHHHHEEEECHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCEECCCHHHHHCC
L
C
>Mature Secondary Structure
MITIEHLLTILKKDHNFREVLDQDGYHFNYSGLQFDSLSYDSRQADEQTLFFAKGATFKA
CEEHHHHHHHHHCCCCHHHHHCCCCCEEECCCEEECCCCCCCCCCCCCEEEEECCCCCHH
DFLKQAIAKGLKIYVSEIDYQVGIPVILVNNIKHAMSLAAMAFYDYPQHKLKLLAFTGTK
HHHHHHHHHHHEEEHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GKTTAAYFAYHMLKESHKPALLSTMNTTLDGKSFFKSQLTTPESLDLFAMMAQCVANGMT
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
HLVMEVSSQAYLVGRVYGLIFDVGVFLNISPDHIGPIEHPSFEDYFYHKRLLMENSRAVV
HHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
VNSGMEHFNILAEQVADKPHDFYGKGACNRLLQRQAFSFQAAGKLAGDYAIQLIGSFNQE
EECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
NAMAAGLACLRLGASLEDIRLGIAKTRVPGRMEVLTMTNQAKIVVDYAHNGDSLEKLLSV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHH
VEEHQKGQIYLILGAPGNKGESRRADFGQVINQHPNVRIILTADDPNFEDPQLISEEIAS
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
YIHREVDIIIDRKEAIKAGMERCQNEQDALIIAGKGADAYQIVKGQKTAYAGDLAIAKSY
HHHHHHHEEEECHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCEECCCHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]