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The map label for this gene is insK [H]

Identifier: 195978622

GI number: 195978622

Start: 1561793

End: 1562629

Strand: Direct

Name: insK [H]

Synonym: Sez_1520

Alternate gene names: 195978622

Gene position: 1561793-1562629 (Clockwise)

Preceding gene: 195978621

Following gene: 195978625

Centisome position: 77.16

GC content: 35.72

Gene sequence:

>837_bases
TTGATCAAAGAATTTAACCTAAGGATATTGCTTGATATTCTGAATCTATCTCGTTCAACGTATTATTACCACCTTAAACG
GTTAGCTCAAAAAGATAAAGATAGCGACTTAAAAAAAACAATTCAAGACATCTATACCGAGCACAAAGGGAGATATGGCT
ACCGTAGAGTACATCTTGAGCTTAAAAATCGAGGGCTTCATGTTAATCATAAAAAAGTTCAGCGCTTAATGACTGAACTT
GGTTTGAAAGCTCGGATTCGTGCGAAACGTCGCTATAATTCCTATAAAGGAGAAATTGGTAAGAAAGCTGATAATCTGAT
TAAGCGTCAATTCAAAGCTACACAACCACTTCAGAAGTGCTACACAGATGTGACTGAATTTTCCATTCCTGCTAGTGACC
AGAAGCTCTATCTCTCACCAGTTCTTGATGGCTATAACAGTGACATTATTGCTTATAATCTCTCTGTATCACCAAACTTA
CAACAACTACAAACAATGCTTAACGAAGCCTTTCCTGAAAAAACTTATCAAGATACTATTTTGCATAGTGATCAAGGTTG
GCAGTATCAACATGTTTACTATCATAATTTTCTTGAGAGCCGTGGAATGAAATCCTCAATGTCTCGTAAGGGAAACAGTT
TAGATAACGGTATGATGGAATCATTCTTTGGAACGCTGAAGACAGAAATGTTTTATGGATTTGAGAAAGAGTTTACTTCT
CTTGAGGATTTGGCAATAGCTATTTCAGAGTATATTGATTATTACAACAACAAACGAATCAAACTAACATTAAAAGGACT
GAGCCCTGTACAATACAGAACTCAATCCTTAGCTTAA

Upstream 100 bases:

>100_bases
ATGAATACCTTAGAGCGGAGATTGCTGTACTAAAAAAGCTGAGAGAATACCGCTTGAGGGACGAGGCAAAGCTCGAAGAG
CAACGGAAATCATTCAGGGA

Downstream 100 bases:

>100_bases
CTATTTTGTCTAACTTTTTGGGGGCATTTCATTTGATAGCTAGTAGCGCAGATGATGTTTCTTTTCCAAGCTCAATAAGA
CTCAAAATTAAGTTCTTTGT

Product: transposase IS861 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIKEFNLRILLDILNLSRSTYYYHLKRLAQKDKDSDLKKTIQDIYTEHKGRYGYRRVHLELKNRGLHVNHKKVQRLMTEL
GLKARIRAKRRYNSYKGEIGKKADNLIKRQFKATQPLQKCYTDVTEFSIPASDQKLYLSPVLDGYNSDIIAYNLSVSPNL
QQLQTMLNEAFPEKTYQDTILHSDQGWQYQHVYYHNFLESRGMKSSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTS
LEDLAIAISEYIDYYNNKRIKLTLKGLSPVQYRTQSLA

Sequences:

>Translated_278_residues
MIKEFNLRILLDILNLSRSTYYYHLKRLAQKDKDSDLKKTIQDIYTEHKGRYGYRRVHLELKNRGLHVNHKKVQRLMTEL
GLKARIRAKRRYNSYKGEIGKKADNLIKRQFKATQPLQKCYTDVTEFSIPASDQKLYLSPVLDGYNSDIIAYNLSVSPNL
QQLQTMLNEAFPEKTYQDTILHSDQGWQYQHVYYHNFLESRGMKSSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTS
LEDLAIAISEYIDYYNNKRIKLTLKGLSPVQYRTQSLA
>Mature_278_residues
MIKEFNLRILLDILNLSRSTYYYHLKRLAQKDKDSDLKKTIQDIYTEHKGRYGYRRVHLELKNRGLHVNHKKVQRLMTEL
GLKARIRAKRRYNSYKGEIGKKADNLIKRQFKATQPLQKCYTDVTEFSIPASDQKLYLSPVLDGYNSDIIAYNLSVSPNL
QQLQTMLNEAFPEKTYQDTILHSDQGWQYQHVYYHNFLESRGMKSSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTS
LEDLAIAISEYIDYYNNKRIKLTLKGLSPVQYRTQSLA

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=243, Percent_Identity=30.0411522633745, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789981, Length=268, Percent_Identity=52.2388059701493, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1788405, Length=281, Percent_Identity=29.5373665480427, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787263, Length=281, Percent_Identity=29.5373665480427, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786753, Length=281, Percent_Identity=29.5373665480427, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786570, Length=281, Percent_Identity=29.5373665480427, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786490, Length=281, Percent_Identity=29.5373665480427, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32740; Mature: 32740

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEFNLRILLDILNLSRSTYYYHLKRLAQKDKDSDLKKTIQDIYTEHKGRYGYRRVHLE
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEE
LKNRGLHVNHKKVQRLMTELGLKARIRAKRRYNSYKGEIGKKADNLIKRQFKATQPLQKC
EECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHH
YTDVTEFSIPASDQKLYLSPVLDGYNSDIIAYNLSVSPNLQQLQTMLNEAFPEKTYQDTI
HHHHHHCCCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHH
LHSDQGWQYQHVYYHNFLESRGMKSSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTS
EECCCCCEEEHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
LEDLAIAISEYIDYYNNKRIKLTLKGLSPVQYRTQSLA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MIKEFNLRILLDILNLSRSTYYYHLKRLAQKDKDSDLKKTIQDIYTEHKGRYGYRRVHLE
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEE
LKNRGLHVNHKKVQRLMTELGLKARIRAKRRYNSYKGEIGKKADNLIKRQFKATQPLQKC
EECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHH
YTDVTEFSIPASDQKLYLSPVLDGYNSDIIAYNLSVSPNLQQLQTMLNEAFPEKTYQDTI
HHHHHHCCCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHH
LHSDQGWQYQHVYYHNFLESRGMKSSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTS
EECCCCCEEEHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
LEDLAIAISEYIDYYNNKRIKLTLKGLSPVQYRTQSLA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]