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The map label for this gene is galS [H]

Identifier: 195978564

GI number: 195978564

Start: 1502352

End: 1503149

Strand: Direct

Name: galS [H]

Synonym: Sez_1462

Alternate gene names: 195978564

Gene position: 1502352-1503149 (Clockwise)

Preceding gene: 195978560

Following gene: 195978565

Centisome position: 74.22

GC content: 39.22

Gene sequence:

>798_bases
ATGCATAAGAAAGTTACGATCAAAGATGTCGCGCGCGAGGCGGGAGTTTCTGTGGCTACTGTCTCCTATGTCATCAATAA
CAGAGAAGATCAAAAAATTAGCCCTGAAACGCGCAATAAAGTGCTGCAAATGATTAATCTTTTAAATTATCGGCCAAACC
AAGCCGCAAAATCACTCGTGACACAAAAAAGACAGCTAATCGCCCTTTACTACAAAGAGCACAGCTCTGTTTTAAAAAGA
GCAGAGCAGCTTCACTTTATTACCCAGCTATCTGCCTTTCTTTACACAAAGCACTATCATTTGATTTGCCTTAATCAAGA
ATACATCGGAAAATATGATGTAGCAGATGTCATTCTCACCCTAGAGGTCACCAAAGAGGAATTTTACAAGATCGGAAATA
GCAACTTTGTTCCTTTGATCGCTATTGATAGTATTATTGATGATCCCTTATTTTTCCAGATTAATAGTGACCCTCATCTC
CTGTTCAATAAAGCTAGTGCTTATTTTGCAGGGGAGCCCTTTGAGCTGGTTAGCTTGCCGATTCAAAACCAAGAAATCAC
TCAGGGTCTCGCTCAACAGTTTGATAACATTTGCTGGATTCACTCTATTTGCGACATCGAAGGTGTGTCTAAGGGGAATA
TTCTAGTTATTGATGATACTGTCAATCATATTCTTAACAAGCACAAGAACAAGCTATACCTGCCAAGTATCTCAGAGGCT
AAGCTTCTGAAGCTTTTTCAGGCTATTGAGTGGGCTTCCAAAAGAGAAGCAATCAACAATCATAATATTTTGGTTTAA

Upstream 100 bases:

>100_bases
TTTCCAGATGTCTCCTATGGCGCTAGCAGTACAAGCTTGTGAGGCTTCCAAGGAGCCGCTTCTAGTTGACTTTTAAGCGT
TTTTTTATTATAACAAAGCT

Downstream 100 bases:

>100_bases
CCGTGATAAGAGCTTAGTCCTATGCTTCAACAAGCTTGCTGCTAAGCTCTTTTTGTCAGTCAAGAGGTCAGCTTTTTCAG
GTATTAGCCGATGTACCAGC

Product: transcriptional regulator LacI family

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MHKKVTIKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLVTQKRQLIALYYKEHSSVLKR
AEQLHFITQLSAFLYTKHYHLICLNQEYIGKYDVADVILTLEVTKEEFYKIGNSNFVPLIAIDSIIDDPLFFQINSDPHL
LFNKASAYFAGEPFELVSLPIQNQEITQGLAQQFDNICWIHSICDIEGVSKGNILVIDDTVNHILNKHKNKLYLPSISEA
KLLKLFQAIEWASKREAINNHNILV

Sequences:

>Translated_265_residues
MHKKVTIKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLVTQKRQLIALYYKEHSSVLKR
AEQLHFITQLSAFLYTKHYHLICLNQEYIGKYDVADVILTLEVTKEEFYKIGNSNFVPLIAIDSIIDDPLFFQINSDPHL
LFNKASAYFAGEPFELVSLPIQNQEITQGLAQQFDNICWIHSICDIEGVSKGNILVIDDTVNHILNKHKNKLYLPSISEA
KLLKLFQAIEWASKREAINNHNILV
>Mature_265_residues
MHKKVTIKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLVTQKRQLIALYYKEHSSVLKR
AEQLHFITQLSAFLYTKHYHLICLNQEYIGKYDVADVILTLEVTKEEFYKIGNSNFVPLIAIDSIIDDPLFFQINSDPHL
LFNKASAYFAGEPFELVSLPIQNQEITQGLAQQFDNICWIHSICDIEGVSKGNILVIDDTVNHILNKHKNKLYLPSISEA
KLLKLFQAIEWASKREAINNHNILV

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=65, Percent_Identity=49.2307692307692, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 30365; Mature: 30365

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKVTIKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLV
CCCEEHHHHHHHHHCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
TQKRQLIALYYKEHSSVLKRAEQLHFITQLSAFLYTKHYHLICLNQEYIGKYDVADVILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHCCCCCHHHEEEE
LEVTKEEFYKIGNSNFVPLIAIDSIIDDPLFFQINSDPHLLFNKASAYFAGEPFELVSLP
EEECHHHHHHCCCCCEEEEEEEHHHHCCCEEEEECCCCCEEEECCCCEECCCCHHEEECC
IQNQEITQGLAQQFDNICWIHSICDIEGVSKGNILVIDDTVNHILNKHKNKLYLPSISEA
CCCHHHHHHHHHHHCCEEEEHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCEEECCCCCHH
KLLKLFQAIEWASKREAINNHNILV
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHKKVTIKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLV
CCCEEHHHHHHHHHCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
TQKRQLIALYYKEHSSVLKRAEQLHFITQLSAFLYTKHYHLICLNQEYIGKYDVADVILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHCCCCCHHHEEEE
LEVTKEEFYKIGNSNFVPLIAIDSIIDDPLFFQINSDPHLLFNKASAYFAGEPFELVSLP
EEECHHHHHHCCCCCEEEEEEEHHHHCCCEEEEECCCCCEEEECCCCEECCCCHHEEECC
IQNQEITQGLAQQFDNICWIHSICDIEGVSKGNILVIDDTVNHILNKHKNKLYLPSISEA
CCCHHHHHHHHHHHCCEEEEHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCEEECCCCCHH
KLLKLFQAIEWASKREAINNHNILV
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3146019 [H]