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The map label for this gene is malE [H]

Identifier: 195978366

GI number: 195978366

Start: 1289778

End: 1291031

Strand: Direct

Name: malE [H]

Synonym: Sez_1257

Alternate gene names: 195978366

Gene position: 1289778-1291031 (Clockwise)

Preceding gene: 195978361

Following gene: 195978367

Centisome position: 63.72

GC content: 42.82

Gene sequence:

>1254_bases
ATGAAATCATGGCAAAAAGTTATCGTCAGTGGGGCAAGCTTGACACTTGCTAGTGCCTTATTAGTAGGGTGTGCATCTGG
TTCAAAGGACAAAACAGAAACTGCATCAGGTACTGATTCAAAAACAATTAAGCTTTGGGTACCAACAGGGGCTAAAAAGT
CATACACAGATGTTGTCGCTAAATTTGAAAAGGACTCTGGCTACAAGGTTAAGGTGATCGAGTCAGAGGATCCAAAAGCA
CAAGAAAAAATCAAAAAAGATGCTTCTACTGCTGCCGATGTCTTTTCACTTCCTCATGACCAGCTTGGTCAGCTCGTAGA
ATCTGGCATTATCCAAGAAGTCCCAGAGGAATACACTAAAGAGATCGCTGATACAGCAACTGATCAAGCTATCCTTGGTG
CTCAATACAAGGGTAAAACCTATGCCTTCCCATTTGGTATTGAGTCACAGGTTCTCTTCTACAACAAAACTAAGCTAGCT
GCTGACGATGTTGCTTCATACGATACCATCACGTCTAAGGCTACCTTTGGTGGTACCTTCAAGCAGGCTAATGCTTATGC
TACTGGTCCATTGTTCATGTCTGTTGGTAACACATTATTTGGTGAAAGCGGTGAAGACACTAAAGGAACTAACTGGGGCA
ATGAAAAAGGGGTTGCTGTTCTTAAATGGATTTCAGACCAAGCCTCAAACAAAGGATTTGTTAACTTAGATGCCAACAAC
GTCATGTCTCAGTTTGGCGACGGTTCAGTTGCTTCATTTGAATCAGGTCCTTGGGACTACGAGGCAGCACAAAAGGCTAT
CGGTAAAGAAAATCTTGGTGTTGCTGTTTATCCAAAGGTAACTATCGGTGGCGAAACCGTTCAACAAAAGGCCTTCTTGG
GAGTGAAGCTCTATGCGGTTAACCAAGCTCCTGCTAAGGGAGATACCAAGCGTATCGCAGCCAGCTACAAGCTTGCTTCA
TACCTAACAAATGCTGAAAGCCAAGAAAACCAATTCAAGACACGTAACATCGTACCAGCTAACAAGACCGTTCAAGCTTC
AGAGGCTGTTCAATCAAACGAGCTTGCAAAAACAGTTATCACAATGGGATCTTCTTCAGATTATACTGTTGTTATGCCTA
AGCTTAGTCAAATGGGTACCTTCTGGACTGAAAGTGCTGCAATCCTTAGCGATGCCTTCAATGGTAAAATCAAAGAAGGT
GACTACCTTGCTAAGCTACAGCAGTTTGACAAGGACATTGCAGCAACAAAATAA

Upstream 100 bases:

>100_bases
GTTTTAAAAAAATCAACTTTTTTTAAAAAAAGACTTGACTTTTTCTGCAAGCGGTTGCATAATATTAAGTGTAAGAAATA
TATCTTAGGAGGAAATCGCT

Downstream 100 bases:

>100_bases
GCTATCTCATTTATAATACTATCCTAAACAAGTACAGAGGTGGGCTATTTTAACGCTATCCTTTCTACTCATCTCTAAAG
GGGTGGAGCTAAGCATCATT

Product: maltose/maltodextrin ABC transporter, substrate binding periplasmic protein MalE

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MKSWQKVIVSGASLTLASALLVGCASGSKDKTETASGTDSKTIKLWVPTGAKKSYTDVVAKFEKDSGYKVKVIESEDPKA
QEKIKKDASTAADVFSLPHDQLGQLVESGIIQEVPEEYTKEIADTATDQAILGAQYKGKTYAFPFGIESQVLFYNKTKLA
ADDVASYDTITSKATFGGTFKQANAYATGPLFMSVGNTLFGESGEDTKGTNWGNEKGVAVLKWISDQASNKGFVNLDANN
VMSQFGDGSVASFESGPWDYEAAQKAIGKENLGVAVYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLAS
YLTNAESQENQFKTRNIVPANKTVQASEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKEG
DYLAKLQQFDKDIAATK

Sequences:

>Translated_417_residues
MKSWQKVIVSGASLTLASALLVGCASGSKDKTETASGTDSKTIKLWVPTGAKKSYTDVVAKFEKDSGYKVKVIESEDPKA
QEKIKKDASTAADVFSLPHDQLGQLVESGIIQEVPEEYTKEIADTATDQAILGAQYKGKTYAFPFGIESQVLFYNKTKLA
ADDVASYDTITSKATFGGTFKQANAYATGPLFMSVGNTLFGESGEDTKGTNWGNEKGVAVLKWISDQASNKGFVNLDANN
VMSQFGDGSVASFESGPWDYEAAQKAIGKENLGVAVYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLAS
YLTNAESQENQFKTRNIVPANKTVQASEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKEG
DYLAKLQQFDKDIAATK
>Mature_417_residues
MKSWQKVIVSGASLTLASALLVGCASGSKDKTETASGTDSKTIKLWVPTGAKKSYTDVVAKFEKDSGYKVKVIESEDPKA
QEKIKKDASTAADVFSLPHDQLGQLVESGIIQEVPEEYTKEIADTATDQAILGAQYKGKTYAFPFGIESQVLFYNKTKLA
ADDVASYDTITSKATFGGTFKQANAYATGPLFMSVGNTLFGESGEDTKGTNWGNEKGVAVLKWISDQASNKGFVNLDANN
VMSQFGDGSVASFESGPWDYEAAQKAIGKENLGVAVYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLAS
YLTNAESQENQFKTRNIVPANKTVQASEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKEG
DYLAKLQQFDKDIAATK

Specific function: Binding protein for maltose and maltodextrin; involved in its cellular uptake [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=405, Percent_Identity=26.9135802469136, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44698; Mature: 44698

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWQKVIVSGASLTLASALLVGCASGSKDKTETASGTDSKTIKLWVPTGAKKSYTDVVA
CCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
KFEKDSGYKVKVIESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGIIQEVPEEYTK
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EIADTATDQAILGAQYKGKTYAFPFGIESQVLFYNKTKLAADDVASYDTITSKATFGGTF
HHHHHHCCHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCH
KQANAYATGPLFMSVGNTLFGESGEDTKGTNWGNEKGVAVLKWISDQASNKGFVNLDANN
HCCCCEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEEEECHHH
VMSQFGDGSVASFESGPWDYEAAQKAIGKENLGVAVYPKVTIGGETVQQKAFLGVKLYAV
HHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHEEEEEEEE
NQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKTVQASEAVQSNELAKTVI
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
TMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKEGDYLAKLQQFDKDIAATK
EECCCCCEEEECCCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSWQKVIVSGASLTLASALLVGCASGSKDKTETASGTDSKTIKLWVPTGAKKSYTDVVA
CCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
KFEKDSGYKVKVIESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGIIQEVPEEYTK
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EIADTATDQAILGAQYKGKTYAFPFGIESQVLFYNKTKLAADDVASYDTITSKATFGGTF
HHHHHHCCHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCH
KQANAYATGPLFMSVGNTLFGESGEDTKGTNWGNEKGVAVLKWISDQASNKGFVNLDANN
HCCCCEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEEEECHHH
VMSQFGDGSVASFESGPWDYEAAQKAIGKENLGVAVYPKVTIGGETVQQKAFLGVKLYAV
HHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHEEEEEEEE
NQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKTVQASEAVQSNELAKTVI
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
TMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKEGDYLAKLQQFDKDIAATK
EECCCCCEEEECCCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 2684766 [H]