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The map label for this gene is citF [H]

Identifier: 195978121

GI number: 195978121

Start: 1034040

End: 1035572

Strand: Direct

Name: citF [H]

Synonym: Sez_0996

Alternate gene names: 195978121

Gene position: 1034040-1035572 (Clockwise)

Preceding gene: 195978120

Following gene: 195978122

Centisome position: 51.08

GC content: 42.73

Gene sequence:

>1533_bases
ATGGTTAAAAATAAACTTGGTCGCGATATTCCTCAGGAGTATGCTGATCAATATGGGGTATTTGAAGGAGAGCTAGCACG
TATCACTAGCTACAATGAATCAAGCCGCCGCATCAAGCCTGTAAAACCAGGTGACAGTAAATTACTTGGGTCTATTCGTG
AAGCTATTGAAAAAACAGGTCTAAAAGATGGCATGACCATTTCATTTCACCATCATTTTCGTGAAGGTGATTTCGTCATG
AATATGGTTCTTGATGAGATAGCCAGCATGGGGATCAAGGATTTAGCTATCGCTCCAAGCTCTATTGCCAATGTGCATGA
GCCATTAATTGAGCACATCAAAAACGGAGTGGTTACAAGCATTACCTCATCAGGTTTACGTGATAAGGTTGGTGCAGCTA
TTTCATCAGGGATTATGGAAAAGCCTGTTGTGATCCGTTCTCATGGAGGACGTGCTCGTGCGATTGCTAGTGGTGATATT
CATATTGACGTTGCCTTTTTAGGAGCACCAAGCTCAGATGAATATGGTAATGTCAACGGTACGATTGGTAAGGCTACCTG
CGGCTCACTTGGCTATGCTATGATCGATGCCAAGTACGCAGATCAGGTGGTGGTGCTGACAGATCACCTAGTTGATTATC
CTAACACCCCAATTAGCATTCCGCAAACTGATGTTGATTATATCGTGACAGTTGATGCTATTGGTGATCCACAAGGAATC
GCTAAGGGAGCAACGCGGTTCACCAAAAATCCTAAGGAGCTCCTAATTGCCGAATATGCCGCTAAGGTTATCACACAGTC
GCCTTACTTTAAAAATGGCTTTTCCTTCCAAACAGGTACAGGAGGAGCTTCCTTAGCAGTGACTAGGTTCATGCGTGAGG
CCATGCTTCAAGAAAATATCAAGGCAAGCTTTGCCCTTGGAGGCATTACAAATGCTATGGTAGAATTGCTCGAAGAAGGA
TTAGTTGAGAAAATCCTAGATGTACAGGATTTTGATTACCCTTCAGCAGTTTCGCTTGGTAAAAATGCTCAGCATTACGA
AATTGATGCTAACATGTATGCCTCACCACTAAGCAAGGGATCGGTTATCAATCAATTAGACACCTGTATTTTATCAGCGC
TTGAGGTTGACACTAATTTCAATGTTAATGTCATGACAGGTTCTGACGGTGTTATTCGTGGAGCTTCCGGAGGGCATTGT
GACACAGCCTTTGCTGCCAAAATGAGTCTGGTGATTTCACCCCTTATCCGCGGACGCATTCCAACCTTTGTAGATGAGGT
AAATACAGTAATTACACCTGGAACAAGCGTTGATGTGGTTGTCACAGAAATAGGTATTGCTATCAATCCAAACCGTCCAG
ATTTGATAGAGCATTTCAAGGACTTGAAAGTTCCTCAATTTAGTATCGAAGAATTAAAAGAAAAGGCCTACTCGATTGTT
GGAACACCTGATTGTATCCAATACGGTGATAAGGTTGTAGCTCTTATTGAGTATCGTGATGGCAGCCTGATTGACGTTGT
CTACAATGTCTAA

Upstream 100 bases:

>100_bases
TTAAATGGAAAAATGGTAGATAAGCCAATCGTAGAGCGTGCACAGCGTGTTATCGCTTTGGCAACAGCTGCTGGAGTATT
AACAGAGGAGGACATTTAGC

Downstream 100 bases:

>100_bases
GGAATTGATTTTCAAGGGGAAAGCTGTTACCCTAGCTGATATGCTAAGGGCACGCGAGGACAGAAGCCTTCGTCAAGGAA
AGCTTTTAACAGAATTCTCA

Product: citrate lyase alpha chain CitF

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MVKNKLGRDIPQEYADQYGVFEGELARITSYNESSRRIKPVKPGDSKLLGSIREAIEKTGLKDGMTISFHHHFREGDFVM
NMVLDEIASMGIKDLAIAPSSIANVHEPLIEHIKNGVVTSITSSGLRDKVGAAISSGIMEKPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDEYGNVNGTIGKATCGSLGYAMIDAKYADQVVVLTDHLVDYPNTPISIPQTDVDYIVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITQSPYFKNGFSFQTGTGGASLAVTRFMREAMLQENIKASFALGGITNAMVELLEEG
LVEKILDVQDFDYPSAVSLGKNAQHYEIDANMYASPLSKGSVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEIGIAINPNRPDLIEHFKDLKVPQFSIEELKEKAYSIV
GTPDCIQYGDKVVALIEYRDGSLIDVVYNV

Sequences:

>Translated_510_residues
MVKNKLGRDIPQEYADQYGVFEGELARITSYNESSRRIKPVKPGDSKLLGSIREAIEKTGLKDGMTISFHHHFREGDFVM
NMVLDEIASMGIKDLAIAPSSIANVHEPLIEHIKNGVVTSITSSGLRDKVGAAISSGIMEKPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDEYGNVNGTIGKATCGSLGYAMIDAKYADQVVVLTDHLVDYPNTPISIPQTDVDYIVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITQSPYFKNGFSFQTGTGGASLAVTRFMREAMLQENIKASFALGGITNAMVELLEEG
LVEKILDVQDFDYPSAVSLGKNAQHYEIDANMYASPLSKGSVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEIGIAINPNRPDLIEHFKDLKVPQFSIEELKEKAYSIV
GTPDCIQYGDKVVALIEYRDGSLIDVVYNV
>Mature_510_residues
MVKNKLGRDIPQEYADQYGVFEGELARITSYNESSRRIKPVKPGDSKLLGSIREAIEKTGLKDGMTISFHHHFREGDFVM
NMVLDEIASMGIKDLAIAPSSIANVHEPLIEHIKNGVVTSITSSGLRDKVGAAISSGIMEKPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDEYGNVNGTIGKATCGSLGYAMIDAKYADQVVVLTDHLVDYPNTPISIPQTDVDYIVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITQSPYFKNGFSFQTGTGGASLAVTRFMREAMLQENIKASFALGGITNAMVELLEEG
LVEKILDVQDFDYPSAVSLGKNAQHYEIDANMYASPLSKGSVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEIGIAINPNRPDLIEHFKDLKVPQFSIEELKEKAYSIV
GTPDCIQYGDKVVALIEYRDGSLIDVVYNV

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=469, Percent_Identity=57.7825159914712, Blast_Score=569, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54938; Mature: 54938

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNKLGRDIPQEYADQYGVFEGELARITSYNESSRRIKPVKPGDSKLLGSIREAIEKTG
CCCCCCCCCCHHHHHHHCCCCCCCHHHEECCCCCCCEECCCCCCHHHHHHHHHHHHHHCC
LKDGMTISFHHHFREGDFVMNMVLDEIASMGIKDLAIAPSSIANVHEPLIEHIKNGVVTS
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHHHHCCEEEH
ITSSGLRDKVGAAISSGIMEKPVVIRSHGGRARAIASGDIHIDVAFLGAPSSDEYGNVNG
HHHCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
TIGKATCGSLGYAMIDAKYADQVVVLTDHLVDYPNTPISIPQTDVDYIVTVDAIGDPQGI
CCCCCHHCCCCHHEEEHHHCCEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHH
AKGATRFTKNPKELLIAEYAAKVITQSPYFKNGFSFQTGTGGASLAVTRFMREAMLQENI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCC
KASFALGGITNAMVELLEEGLVEKILDVQDFDYPSAVSLGKNAQHYEIDANMYASPLSKG
HHHEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEECCCHHCCCCCCC
SVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHCDTAFAAKMSLVISPLIRGRI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
PTFVDEVNTVITPGTSVDVVVTEIGIAINPNRPDLIEHFKDLKVPQFSIEELKEKAYSIV
CHHHHCCCCEECCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEC
GTPDCIQYGDKVVALIEYRDGSLIDVVYNV
CCCHHHHCCCEEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MVKNKLGRDIPQEYADQYGVFEGELARITSYNESSRRIKPVKPGDSKLLGSIREAIEKTG
CCCCCCCCCCHHHHHHHCCCCCCCHHHEECCCCCCCEECCCCCCHHHHHHHHHHHHHHCC
LKDGMTISFHHHFREGDFVMNMVLDEIASMGIKDLAIAPSSIANVHEPLIEHIKNGVVTS
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHHHHCCEEEH
ITSSGLRDKVGAAISSGIMEKPVVIRSHGGRARAIASGDIHIDVAFLGAPSSDEYGNVNG
HHHCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
TIGKATCGSLGYAMIDAKYADQVVVLTDHLVDYPNTPISIPQTDVDYIVTVDAIGDPQGI
CCCCCHHCCCCHHEEEHHHCCEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHH
AKGATRFTKNPKELLIAEYAAKVITQSPYFKNGFSFQTGTGGASLAVTRFMREAMLQENI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCC
KASFALGGITNAMVELLEEGLVEKILDVQDFDYPSAVSLGKNAQHYEIDANMYASPLSKG
HHHEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEECCCHHCCCCCCC
SVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHCDTAFAAKMSLVISPLIRGRI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
PTFVDEVNTVITPGTSVDVVVTEIGIAINPNRPDLIEHFKDLKVPQFSIEELKEKAYSIV
CHHHHCCCCEECCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEC
GTPDCIQYGDKVVALIEYRDGSLIDVVYNV
CCCHHHHCCCEEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]