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The map label for this gene is citE [H]

Identifier: 195978120

GI number: 195978120

Start: 1033175

End: 1034038

Strand: Direct

Name: citE [H]

Synonym: Sez_0995

Alternate gene names: 195978120

Gene position: 1033175-1034038 (Clockwise)

Preceding gene: 195978119

Following gene: 195978121

Centisome position: 51.04

GC content: 45.37

Gene sequence:

>864_bases
ATGTTTGTTCCAGGCGCTAATGCGGCTATGCTAAGAGACGCACCCTTGTTTGGAGCGGACTCTATCATGTTTGATTTAGA
AGATTCAGTTTCCTTAAAAGAAAAAGACACCTCTCGCGCCCTTGTTCATTTTGCGCTAAAAACCTTTGATTATTCAAATG
TTGAGACAGTTGTGCGCGTTAATGGCCTAGACTCTTGTGGTGCTCTGGATATTGAGGCAGTTGTGTTAGCAGGTGTTGAT
GTTATTCGCCTGCCAAAGACAGAAACTGCCCAGGATATTATTGATGTTGAGGCAGTCATTGAGCGTGTCGAACGTGACAA
TGGGATTGCTGTCGGTCGTACACGCATGATGGCGGCTATCGAGTCTGCAGAAGGGGTCTTAAATGCTCGTGAGATTGCAA
AGGCCTCAGACCGTCTCATTGGTATCGCCCTTGGTGCAGAGGATTATGTGACCAATATGAAAACACGTCGCTACCCAGAC
GGACAAGAATTATTCTTTGCCCGTAGCATGATTCTTCACGCAGCACGTGCTGCTGGAATCGCAGCAATTGATACCGTTTA
TTCAGATGTCAACAATACAGAAGGCTTCCAAGCTGAGGTGCGTCATATCAAGCAATTAGGTTTTGATGGCAAATCTGTCA
TTAACCCTCGTCAGATTCCATTGGTAAATGAGATTTACACGCCAACCGAAAAAGAGATTAGTCATGCCAAGCAAGTGATC
TGGGCCATTCGTGAGGCTGAAAGTAAAGGATCAGGTGTCATCTCATTAAATGGAAAAATGGTAGATAAGCCAATCGTAGA
GCGTGCACAGCGTGTTATCGCTTTGGCAACAGCTGCTGGAGTATTAACAGAGGAGGACATTTAG

Upstream 100 bases:

>100_bases
CCATTCAGGCAAGAACAATTGCAGCAGTCCATCGTGCTGCTGGAGTTGAGCATTACAACTGGAAGGAGATCGACTCATGG
AACGCTTAAGAAGAACGATG

Downstream 100 bases:

>100_bases
CATGGTTAAAAATAAACTTGGTCGCGATATTCCTCAGGAGTATGCTGATCAATATGGGGTATTTGAAGGAGAGCTAGCAC
GTATCACTAGCTACAATGAA

Product: citrate lyase beta chain CitE

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSNVETVVRVNGLDSCGALDIEAVVLAGVD
VIRLPKTETAQDIIDVEAVIERVERDNGIAVGRTRMMAAIESAEGVLNAREIAKASDRLIGIALGAEDYVTNMKTRRYPD
GQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQAEVRHIKQLGFDGKSVINPRQIPLVNEIYTPTEKEISHAKQVI
WAIREAESKGSGVISLNGKMVDKPIVERAQRVIALATAAGVLTEEDI

Sequences:

>Translated_287_residues
MFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSNVETVVRVNGLDSCGALDIEAVVLAGVD
VIRLPKTETAQDIIDVEAVIERVERDNGIAVGRTRMMAAIESAEGVLNAREIAKASDRLIGIALGAEDYVTNMKTRRYPD
GQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQAEVRHIKQLGFDGKSVINPRQIPLVNEIYTPTEKEISHAKQVI
WAIREAESKGSGVISLNGKMVDKPIVERAQRVIALATAAGVLTEEDI
>Mature_287_residues
MFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSNVETVVRVNGLDSCGALDIEAVVLAGVD
VIRLPKTETAQDIIDVEAVIERVERDNGIAVGRTRMMAAIESAEGVLNAREIAKASDRLIGIALGAEDYVTNMKTRRYPD
GQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQAEVRHIKQLGFDGKSVINPRQIPLVNEIYTPTEKEISHAKQVI
WAIREAESKGSGVISLNGKMVDKPIVERAQRVIALATAAGVLTEEDI

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=27.1186440677966, Blast_Score=124, Evalue=9e-29,
Organism=Escherichia coli, GI87081764, Length=285, Percent_Identity=57.8947368421053, Blast_Score=321, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17557458, Length=291, Percent_Identity=28.1786941580756, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 31184; Mature: 31184

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSNVETVVRV
CCCCCCCCHHEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEE
NGLDSCGALDIEAVVLAGVDVIRLPKTETAQDIIDVEAVIERVERDNGIAVGRTRMMAAI
CCCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
ESAEGVLNAREIAKASDRLIGIALGAEDYVTNMKTRRYPDGQELFFARSMILHAARAAGI
HHHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHCCH
AAIDTVYSDVNNTEGFQAEVRHIKQLGFDGKSVINPRQIPLVNEIYTPTEKEISHAKQVI
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
WAIREAESKGSGVISLNGKMVDKPIVERAQRVIALATAAGVLTEEDI
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSNVETVVRV
CCCCCCCCHHEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEE
NGLDSCGALDIEAVVLAGVDVIRLPKTETAQDIIDVEAVIERVERDNGIAVGRTRMMAAI
CCCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
ESAEGVLNAREIAKASDRLIGIALGAEDYVTNMKTRRYPDGQELFFARSMILHAARAAGI
HHHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHCCH
AAIDTVYSDVNNTEGFQAEVRHIKQLGFDGKSVINPRQIPLVNEIYTPTEKEISHAKQVI
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
WAIREAESKGSGVISLNGKMVDKPIVERAQRVIALATAAGVLTEEDI
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457870 [H]