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The map label for this gene is pyk [H]

Identifier: 195978009

GI number: 195978009

Start: 925545

End: 927047

Strand: Direct

Name: pyk [H]

Synonym: Sez_0882

Alternate gene names: 195978009

Gene position: 925545-927047 (Clockwise)

Preceding gene: 195978008

Following gene: 195978010

Centisome position: 45.72

GC content: 42.05

Gene sequence:

>1503_bases
ATGAATAAACGTGTAAAAATTGTTGCAACACTTGGTCCTGCGGTTGAAATTCGCGGTGGTAAAAAATATGGTGAGGACGG
CTATTGGGCTGGTCAGTTGGACGTTGAACAATCAGCTAAAAAAATCGCTGAATTGATTGAAGCAGGTGCTAACGTTTTCC
GCTTTAACTTTTCACATGGTGACCACAAAGAGCAGGGTGACCGTATGGCTACTGTTCGTCTTGCTGAAAAGCTGGCACGT
CAAAAGGTTGGTTTCCTTTTGGATACAAAAGGACCAGAAATGCGTACAGAATTGTTTGCTGATGATGCCAAAGAATATAG
CTATGTGACTGGTGAGCGTATTCGTGTTGCGACAGCACAAGGCATTCAATCAACTCGTGATGTGATTGCTTTGAATGTAG
CTGGTGCTCTTGATATTTATGATGAGGTTGAGGTTGGCCACACTATTCTTATTGACGATGGAAAGCTTGGCCTAAAGGTT
ATCGAAAAGGACATTGCAAAGCGTGAGTTTATCGTTGAGGTTGAAAATGATGGCATCATTGCAAAGCAAAAGGGTGTTAA
TATTCCTAATACTAAGATTCCTTTCCCAGCCTTAGCAGAACGTGACAATGCTGATATTCGTTTTGGTCTTGAGCAAGGTC
TTAACTTTATTGCTATTTCATTTGTTCGTACTGCCAAGGACGTTGAAGAGGTTCGTGAGATTTGTCGTGAAACAGGCAAT
GAGCATGTTCAATTATTTGCTAAAATCGAAAACCAACAAGGTATTGATAACCTTGATGAAATCATCGAAGCTGCAGATGG
TATCATGATTGCTCGTGGTGATATGGGGATTGAGGTGCCATTTGAAATGGTTCCAGTCTTCCAAAAAATGATTATCACCA
AGGTAAATGCAGCAGGTAAAGCAGTCATCACCGCAACTAACATGCTTGAAACAATGACTGAAAAACCACGTGCAACTCGT
TCAGAGGTATCTGACGTTTTCAACGCGGTTATTGACGGTACAGATGCGACAATGCTTTCTGGTGAGTCAGCAAATGGTAA
ATACCCTGTTGAGGCAGTTCGTACAATGGCTACAATTGATAAAAATGCACAAACTCTTCTTAATGAGTATGGTCGTTTAG
ACTCATCTGCCTTCCCACGTACCAATAAAACTGACGTGATTGCTTCAGCTGTTAAAGATGCAACACACTCAATGGATATC
AAGCTTGTTGTGACCATCACTGAAACAGGAAACACAGCTCGTGCCATTTCTAAGTTCCGTCCTGATGCTGACATCTTGGC
TGTGACCTTTGATGAAAAGGTTCAACGTGCCTTGATGATTAACTGGGGTGTGATTCCAGTGCTTGCTGAAAGACCAGCAT
CAACAGATGATATGTTTGAGGTAGCAGAGCGTGTAGCTCTTGAGACTGGTTTGGTGCAATCAGGAGATAATATCGTTATT
GTTGCAGGTGTGCCAGTTGGTACAGGTGGTACAAATACAATGCGTGTTCGCACTGTAAAATAA

Upstream 100 bases:

>100_bases
TTAGACTATGCTGCTTTGAACCGTTCATTAGCGCAATAGGATAAGTAAGATCTGGTAATCTTTGTTAATCAACTAAAAAA
TAAGGGAGTTTCATAATAAA

Downstream 100 bases:

>100_bases
TAGCTTTCCATAAGCTTTAGCTATTTTGCTCTATCAGATAATTTAAGGCAAACAATGATACTTAAAAATTGTTTGCTTTT
TTTATTAGTCATATCATGTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEQSAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEKLAR
QKVGFLLDTKGPEMRTELFADDAKEYSYVTGERIRVATAQGIQSTRDVIALNVAGALDIYDEVEVGHTILIDDGKLGLKV
IEKDIAKREFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVEAVRTMATIDKNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAERPASTDDMFEVAERVALETGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK

Sequences:

>Translated_500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEQSAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEKLAR
QKVGFLLDTKGPEMRTELFADDAKEYSYVTGERIRVATAQGIQSTRDVIALNVAGALDIYDEVEVGHTILIDDGKLGLKV
IEKDIAKREFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVEAVRTMATIDKNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAERPASTDDMFEVAERVALETGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK
>Mature_500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEQSAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEKLAR
QKVGFLLDTKGPEMRTELFADDAKEYSYVTGERIRVATAQGIQSTRDVIALNVAGALDIYDEVEVGHTILIDDGKLGLKV
IEKDIAKREFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVEAVRTMATIDKNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAERPASTDDMFEVAERVALETGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=515, Percent_Identity=36.8932038834951, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI33286420, Length=515, Percent_Identity=36.8932038834951, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI33286418, Length=515, Percent_Identity=36.8932038834951, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI32967597, Length=514, Percent_Identity=36.1867704280156, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI10835121, Length=514, Percent_Identity=36.3813229571984, Blast_Score=308, Evalue=9e-84,
Organism=Homo sapiens, GI310128732, Length=311, Percent_Identity=37.2990353697749, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128730, Length=311, Percent_Identity=37.2990353697749, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128736, Length=259, Percent_Identity=32.8185328185328, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI310128734, Length=259, Percent_Identity=32.8185328185328, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI310128738, Length=221, Percent_Identity=35.2941176470588, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787965, Length=501, Percent_Identity=40.7185628742515, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788160, Length=511, Percent_Identity=33.2681017612524, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17544584, Length=505, Percent_Identity=38.4158415841584, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17506831, Length=511, Percent_Identity=38.9432485322896, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71984413, Length=513, Percent_Identity=38.7914230019493, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=513, Percent_Identity=38.7914230019493, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=513, Percent_Identity=38.7914230019493, Blast_Score=298, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=507, Percent_Identity=35.8974358974359, Blast_Score=298, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6324923, Length=509, Percent_Identity=37.1316306483301, Blast_Score=296, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24648964, Length=519, Percent_Identity=37.1868978805395, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI28571814, Length=519, Percent_Identity=37.1868978805395, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=37.9227053140097, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24581235, Length=475, Percent_Identity=32.2105263157895, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24646914, Length=343, Percent_Identity=32.6530612244898, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54616; Mature: 54616

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEQSAKKIAELIEAGANVFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCC
DHKEQGDRMATVRLAEKLARQKVGFLLDTKGPEMRTELFADDAKEYSYVTGERIRVATAQ
CCCHHCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCHHHCEEECCCEEEEEEHH
GIQSTRDVIALNVAGALDIYDEVEVGHTILIDDGKLGLKVIEKDIAKREFIVEVENDGII
CCHHCCCEEEEEECCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCC
EHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
AVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVEAVRTMATID
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHC
KNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDIKLVVTITETGNTARAISKFR
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHC
PDADILAVTFDEKVQRALMINWGVIPVLAERPASTDDMFEVAERVALETGLVQSGDNIVI
CCCCEEEEEEHHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCEECCCCEEE
VAGVPVGTGGTNTMRVRTVK
EEECCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEQSAKKIAELIEAGANVFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCC
DHKEQGDRMATVRLAEKLARQKVGFLLDTKGPEMRTELFADDAKEYSYVTGERIRVATAQ
CCCHHCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCHHHCEEECCCEEEEEEHH
GIQSTRDVIALNVAGALDIYDEVEVGHTILIDDGKLGLKVIEKDIAKREFIVEVENDGII
CCHHCCCEEEEEECCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCC
EHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
AVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVEAVRTMATID
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHC
KNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDIKLVVTITETGNTARAISKFR
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHC
PDADILAVTFDEKVQRALMINWGVIPVLAERPASTDDMFEVAERVALETGLVQSGDNIVI
CCCCEEEEEEHHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCEECCCCEEE
VAGVPVGTGGTNTMRVRTVK
EEECCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471 [H]