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The map label for this gene is pfkA

Identifier: 195978008

GI number: 195978008

Start: 924470

End: 925483

Strand: Direct

Name: pfkA

Synonym: Sez_0881

Alternate gene names: 195978008

Gene position: 924470-925483 (Clockwise)

Preceding gene: 195978007

Following gene: 195978009

Centisome position: 45.67

GC content: 46.65

Gene sequence:

>1014_bases
ATGAAACGTATTGCTGTTTTAACCAGTGGTGGTGACGCCCCTGGTATGAATGCTGCTATTCGTGCAGTTGTTCGTAAAGC
AATTTCAGAAGGAATGGAAGTCTATGGTATTAACCGCGGCTATGCTGGTATGGTTGATGGTGATATCTTTCCACTAGGCT
CAAAAGAGGTTGGTGATAAGATTTCTCGCGGCGGGACCTTCCTTTATTCAGCTCGCTACCCAGAGTTTGCTCAGCTTGAG
GGGCAATTGGCTGGTATTGAGCAGCTAAAAAAGCATGGCATTGAAGGGGTAGTTGTCATTGGTGGTGATGGTTCTTACCA
TGGTGCGATGCGCTTGACTGAGCATGGCTTCCCTGCAGTAGGTATCCCAGGGACGATTGATAACGATATTGCTGGAACAG
ACTATACTATCGGATTTGACACTGCGGTCAATACTGCGGTAGAGGCTATTGATAAGCTTCGCGATACCTCATCAAGTCAT
GGACGGACCTTTGTTGTTGAAGTGATGGGACGTAATGCAGGTGATATCGCACTTTGGGCAGGTATTGCAAGTGGAGCAGA
TCAAATCATCGTTCCTGAAGAGGAATTTGACATTCATAAAGTGGTCTCAACAATTAAAGACGATTTTGAAAATAGAGGAA
AAAATCACCATATTATCGTTTTAGCTGAGGGTGTTATGAGTGGCGAGGCCTTTGCGCAGCAGCTAAAAGAAGCTGGAGAT
GAAAGTGACCTTCGTGTGACAAACCTTGGTCATATCCTTCGCGGTGGCTCTCCAACTGCGCGTGATCGTGTGATTGCGTC
ATGGATGGGAGCGCACGCTGTTGAGCTGCTCAAGGAAGGCAAAGGCGGCTTGGCTGTTGGTATTCATAATGAAGAGCTTG
TTGAAAGTCCGATTCTTGGAAGTGCAGAGGACGGTGCCTTGTTCAGCCTTACTGATGAGGGTAACATTGTTGTTAATAAT
CCGCACAAGGCTCGCTTAGACTATGCTGCTTTGAACCGTTCATTAGCGCAATAG

Upstream 100 bases:

>100_bases
TTGAAAACGGTTTTTCGATAAAAATAGCGAATTAGTGAAGTAATTTGCAAACGAATGTGCTATAATAAAAACGTTAAATT
AATTAAAGGAGTACCAAAAA

Downstream 100 bases:

>100_bases
GATAAGTAAGATCTGGTAATCTTTGTTAATCAACTAAAAAATAAGGGAGTTTCATAATAAAATGAATAAACGTGTAAAAA
TTGTTGCAACACTTGGTCCT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIHKVVSTIKDDFENRGKNHHIIVLAEGVMSGEAFAQQLKEAGD
ESDLRVTNLGHILRGGSPTARDRVIASWMGAHAVELLKEGKGGLAVGIHNEELVESPILGSAEDGALFSLTDEGNIVVNN
PHKARLDYAALNRSLAQ

Sequences:

>Translated_337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIHKVVSTIKDDFENRGKNHHIIVLAEGVMSGEAFAQQLKEAGD
ESDLRVTNLGHILRGGSPTARDRVIASWMGAHAVELLKEGKGGLAVGIHNEELVESPILGSAEDGALFSLTDEGNIVVNN
PHKARLDYAALNRSLAQ
>Mature_337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIHKVVSTIKDDFENRGKNHHIIVLAEGVMSGEAFAQQLKEAGD
ESDLRVTNLGHILRGGSPTARDRVIASWMGAHAVELLKEGKGGLAVGIHNEELVESPILGSAEDGALFSLTDEGNIVVNN
PHKARLDYAALNRSLAQ

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=339, Percent_Identity=38.9380530973451, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI48762920, Length=317, Percent_Identity=40.0630914826498, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI266453619, Length=340, Percent_Identity=37.6470588235294, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI266453768, Length=340, Percent_Identity=37.6470588235294, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI266453748, Length=340, Percent_Identity=37.6470588235294, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI4505749, Length=340, Percent_Identity=37.6470588235294, Blast_Score=188, Evalue=5e-48,
Organism=Escherichia coli, GI1790350, Length=305, Percent_Identity=56.3934426229508, Blast_Score=318, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25147584, Length=339, Percent_Identity=38.0530973451327, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17558788, Length=314, Percent_Identity=36.624203821656, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=32.5259515570934, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=340, Percent_Identity=37.6470588235294, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=35.1906158357771, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI28573326, Length=338, Percent_Identity=39.0532544378698, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24652337, Length=339, Percent_Identity=38.9380530973451, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI17647809, Length=339, Percent_Identity=38.9380530973451, Blast_Score=201, Evalue=7e-52,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_STRE4 (C0M8V6)

Other databases:

- EMBL:   FM204883
- RefSeq:   YP_002746332.1
- ProteinModelPortal:   C0M8V6
- EnsemblBacteria:   EBSTRT00000067295
- GeneID:   7696893
- GenomeReviews:   FM204883_GR
- KEGG:   seu:SEQ_1011
- GeneTree:   EBGT00050000027028
- OMA:   RCEEFKT
- ProtClustDB:   PRK03202
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35671; Mature: 35671

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 245-245 BINDING 251-251 BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHH
ISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAV
HHCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSHGRTFVVEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GIASGADQIIVPEEEFDIHKVVSTIKDDFENRGKNHHIIVLAEGVMSGEAFAQQLKEAGD
ECCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCC
ESDLRVTNLGHILRGGSPTARDRVIASWMGAHAVELLKEGKGGLAVGIHNEELVESPILG
CCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCC
SAEDGALFSLTDEGNIVVNNPHKARLDYAALNRSLAQ
CCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHH
ISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAV
HHCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSHGRTFVVEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GIASGADQIIVPEEEFDIHKVVSTIKDDFENRGKNHHIIVLAEGVMSGEAFAQQLKEAGD
ECCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCC
ESDLRVTNLGHILRGGSPTARDRVIASWMGAHAVELLKEGKGGLAVGIHNEELVESPILG
CCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCC
SAEDGALFSLTDEGNIVVNNPHKARLDYAALNRSLAQ
CCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA