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The map label for this gene is spsK [H]

Identifier: 195977965

GI number: 195977965

Start: 880688

End: 881536

Strand: Direct

Name: spsK [H]

Synonym: Sez_0837

Alternate gene names: 195977965

Gene position: 880688-881536 (Clockwise)

Preceding gene: 195977964

Following gene: 195977966

Centisome position: 43.51

GC content: 45.23

Gene sequence:

>849_bases
ATGATTTTAATTACGGGAAGCAATGGTCAATTAGGAACAGAGCTTCGTTATCTTTTAGACGAACGAAGCGTCGATTATGT
TGCTGTTGATGTTGCTGAAATGGATATTACCAACGCGACTAAAGTAGAGGAGGTCTTTGCTCAGCTCAAGCCTAGCTTGG
TTTATCACTGTGCAGCCTACACAGCAGTTGATGCTGCTGAAGAAGAAGGAAAAGCACTGAATGAAGCCATCAACGTAGCT
GGTACTGAGCATATTGCCAAAGCTTGTGAGCGCTACGGGGCGACCTTGGTTTATATTTCAACGGATTATGTTTTTGATGG
CAATAAGCCAGCTGGTCAAGAGTGGCTGGAAACCGATGTGCCTGATCCTCAAACAGCATACGGTCGTGCTAAGCGCCTAG
GTGAGCTGGCGGTTGAGCGATACGCTAAGCAATATTACATTATTCGTACCGCATGGGTATTTGGTCATTATGGTAAGAAT
TTTGTGTTTACCATGCAAAACCTTGCTAAGACCCACTCTCGCTTGACAGTCGTCAATGATCAGCATGGGCGTCCAACCTG
GACGAGGACCTTGGCTGAGTTTATGTGTCATCTAGCAGACAATCGCAAGCCTTATGGCTATTATCATTTGTCAAATGATG
CTAAGGAAGACACCACCTGGTATGATTTTGCTAGAGAAATCTTAAAAGAGACAGCAGTCGAGGTTGTGCCTGTGGATTCA
TCAGCTTTTCCTGCTAAGGCCAAAAGGCCCTTTAACTCAACCATGAACCTTGATAAGGCTAAGGCGACTGGTTTTGTGAT
TCCTACCTGGCAAGAAGCCCTAGAGGCATTTGACAAGCAACAGCAATGA

Upstream 100 bases:

>100_bases
CCCTGCCAGGGTCAGTTGATCAAAAAGGCCTCTCTTAGGATTAGATGAGAGTGAGCTGTGTGATTGGCTCAAAGAGATAA
GGCCTTTAAAAGGAGAAAAG

Downstream 100 bases:

>100_bases
ATAAGCGATAGCACTAAAGAGCTGATCTAGGAGATTGGCTCTCTTTTCATGGATTTTATGTCGCATAAGACCTGTTTATA
TGGTATAATACTACTAATTA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MILITGSNGQLGTELRYLLDERSVDYVAVDVAEMDITNATKVEEVFAQLKPSLVYHCAAYTAVDAAEEEGKALNEAINVA
GTEHIAKACERYGATLVYISTDYVFDGNKPAGQEWLETDVPDPQTAYGRAKRLGELAVERYAKQYYIIRTAWVFGHYGKN
FVFTMQNLAKTHSRLTVVNDQHGRPTWTRTLAEFMCHLADNRKPYGYYHLSNDAKEDTTWYDFAREILKETAVEVVPVDS
SAFPAKAKRPFNSTMNLDKAKATGFVIPTWQEALEAFDKQQQ

Sequences:

>Translated_282_residues
MILITGSNGQLGTELRYLLDERSVDYVAVDVAEMDITNATKVEEVFAQLKPSLVYHCAAYTAVDAAEEEGKALNEAINVA
GTEHIAKACERYGATLVYISTDYVFDGNKPAGQEWLETDVPDPQTAYGRAKRLGELAVERYAKQYYIIRTAWVFGHYGKN
FVFTMQNLAKTHSRLTVVNDQHGRPTWTRTLAEFMCHLADNRKPYGYYHLSNDAKEDTTWYDFAREILKETAVEVVPVDS
SAFPAKAKRPFNSTMNLDKAKATGFVIPTWQEALEAFDKQQQ
>Mature_282_residues
MILITGSNGQLGTELRYLLDERSVDYVAVDVAEMDITNATKVEEVFAQLKPSLVYHCAAYTAVDAAEEEGKALNEAINVA
GTEHIAKACERYGATLVYISTDYVFDGNKPAGQEWLETDVPDPQTAYGRAKRLGELAVERYAKQYYIIRTAWVFGHYGKN
FVFTMQNLAKTHSRLTVVNDQHGRPTWTRTLAEFMCHLADNRKPYGYYHLSNDAKEDTTWYDFAREILKETAVEVVPVDS
SAFPAKAKRPFNSTMNLDKAKATGFVIPTWQEALEAFDKQQQ

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=282, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI11034825, Length=282, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=37.6712328767123, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31818; Mature: 31818

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGSNGQLGTELRYLLDERSVDYVAVDVAEMDITNATKVEEVFAQLKPSLVYHCAAY
CEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHHH
TAVDAAEEEGKALNEAINVAGTEHIAKACERYGATLVYISTDYVFDGNKPAGQEWLETDV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCHHHHHCCC
PDPQTAYGRAKRLGELAVERYAKQYYIIRTAWVFGHYGKNFVFTMQNLAKTHSRLTVVND
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHCCCCCCEEEHHHHHHHHCEEEEEEC
QHGRPTWTRTLAEFMCHLADNRKPYGYYHLSNDAKEDTTWYDFAREILKETAVEVVPVDS
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCEEEEECCC
SAFPAKAKRPFNSTMNLDKAKATGFVIPTWQEALEAFDKQQQ
CCCCCCCCCCCCCCCCCCCHHHCCEEEECHHHHHHHHHCCCC
>Mature Secondary Structure
MILITGSNGQLGTELRYLLDERSVDYVAVDVAEMDITNATKVEEVFAQLKPSLVYHCAAY
CEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHHH
TAVDAAEEEGKALNEAINVAGTEHIAKACERYGATLVYISTDYVFDGNKPAGQEWLETDV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCHHHHHCCC
PDPQTAYGRAKRLGELAVERYAKQYYIIRTAWVFGHYGKNFVFTMQNLAKTHSRLTVVND
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHCCCCCCEEEHHHHHHHHCEEEEEEC
QHGRPTWTRTLAEFMCHLADNRKPYGYYHLSNDAKEDTTWYDFAREILKETAVEVVPVDS
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCEEEEECCC
SAFPAKAKRPFNSTMNLDKAKATGFVIPTWQEALEAFDKQQQ
CCCCCCCCCCCCCCCCCCCHHHCCEEEECHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]