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The map label for this gene is icaA [H]

Identifier: 195977955

GI number: 195977955

Start: 865015

End: 866409

Strand: Direct

Name: icaA [H]

Synonym: Sez_0827

Alternate gene names: 195977955

Gene position: 865015-866409 (Clockwise)

Preceding gene: 195977954

Following gene: 195977956

Centisome position: 42.73

GC content: 43.15

Gene sequence:

>1395_bases
GTGCAGCTACTGTTTTTAGTATTGAAATACTCCCAGTATCTTTTTTTAGCGTTTTTGATTGCCTATGCGAGCTTTTTACT
GCTATCCTTAATTGTTGGGGCTATTCACCTTTATGAAAGGCGACAGCAAGTGCCTCTTGGTGGTGATGTTTCTTTACCGC
CAGTGTCTATTTTGGTTCCGGCCTATAATGAAGAGCTAACCATTATCGAAACAGTGGAGTCCTTGCTGATGCTTGATTAT
GACTGCTATGAGATTATTGTCATTGATGATGGGTCAACAGATCATACCGCCAAGCTCTTGAAGGAGCAGTTTCATTTAGA
AGCGGTGACTTGTTCGATTAATCAGCGGATCCCGACACGCCCTCTAAAGGCTGTAAGCCAAAAGCAGCTCAAGCATGTCA
AACTGACCCTGCTTGAAAAGGCAAATGGAGGCAAGGGAGATAGCCTCAATTTTGGGATTAACGCTGCTCAATACGATTAT
TTCTTGTGCATGGACGCTGATTCGATGCTACAGGAGGATTCGCTTAAGCGTATTGCAAAGACTGTTGCAAAGGATCCGTC
TGTCATTGCTGTTGGGGGCATGGTCAAGGTTTCGCAGGGAGTAGCATTGGCAGCTGGGAGGATCGTAGACTACCATTTGC
CGCGTCAGCTTATTTCGTGTGCGCAGGCAATTGAGTATGATTGTGCCTTTTTTGGAGCTCGTGTTTTATTAGACCAGGCT
GCTGGGAATTTAATCATATCAGGAGCGTTTGGACTGTTTAAAAAGGAGCTTGCGATTGCTGTTGATGGTTATGACACTGG
GACTCTGGGAGAGGATATGGAGCTGGTGATGAAATTACATTTTTTCTGCCAGAACAACAAGATTCCTTATCGTATCGGCT
ATGAAACAGACGCTATTTGCTGGAGCCAAGCGCCCAGTCGCTTAAAGGATTTGTGTCAGCAGAGGCGTCGTTGGTTTTTA
GGGCTGTATCAATGCTTGAAAAAGTATCTCCAGTTTTTTGTAAGCGGTCGGTTTAAGGTGACGGTACTCATCTCCTATGT
GCTGTACCTTTTGTTTGAGCTTTTATCTCCTTTTATTGAGGCATTTGGTGTTACTATCATCGTGCTGTCTTTACTATGCC
ATCAATTAAACACGACCTTTTTTGTGTCATTGATGCTGCTCTATATTTGCTATTGTGTGCTGATTGCGCTGACATCGTTT
TTAAATAGGGTTTATTCGCAAAACAATAGTAGCATTACTTTTTTTGATATGGTTTGGGTAATCTACGCTGCTATCTACCA
GTGCCTTGTTCTTCATACGGTATTAACTCTCGTCAAGGTGAGTAGTTTTATTGGCTATCAGCGTAAAAAGAATGCCTGGG
GACAGATCACCAGAGACAAACATAAGGTAGCTTAG

Upstream 100 bases:

>100_bases
TCCTTGTCTTTAACATTGGGTATTTGATGAAGCAGGCCTTGAGACGCTTTTGTCAGGCGAATGGTAAGCACAAGTGATTG
CATGTTAAAGGAGAATGATA

Downstream 100 bases:

>100_bases
CTAGGAGCTCCTAGCCGTTTTTTGGGGTCTATACAAGACAAAGCCTTATCACATTTCTCACGCAAAGCAGCAGACGTGAA
ATGATAGGCTTTTTTCATGC

Product: glycosyl transferase, group 2 family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MQLLFLVLKYSQYLFLAFLIAYASFLLLSLIVGAIHLYERRQQVPLGGDVSLPPVSILVPAYNEELTIIETVESLLMLDY
DCYEIIVIDDGSTDHTAKLLKEQFHLEAVTCSINQRIPTRPLKAVSQKQLKHVKLTLLEKANGGKGDSLNFGINAAQYDY
FLCMDADSMLQEDSLKRIAKTVAKDPSVIAVGGMVKVSQGVALAAGRIVDYHLPRQLISCAQAIEYDCAFFGARVLLDQA
AGNLIISGAFGLFKKELAIAVDGYDTGTLGEDMELVMKLHFFCQNNKIPYRIGYETDAICWSQAPSRLKDLCQQRRRWFL
GLYQCLKKYLQFFVSGRFKVTVLISYVLYLLFELLSPFIEAFGVTIIVLSLLCHQLNTTFFVSLMLLYICYCVLIALTSF
LNRVYSQNNSSITFFDMVWVIYAAIYQCLVLHTVLTLVKVSSFIGYQRKKNAWGQITRDKHKVA

Sequences:

>Translated_464_residues
MQLLFLVLKYSQYLFLAFLIAYASFLLLSLIVGAIHLYERRQQVPLGGDVSLPPVSILVPAYNEELTIIETVESLLMLDY
DCYEIIVIDDGSTDHTAKLLKEQFHLEAVTCSINQRIPTRPLKAVSQKQLKHVKLTLLEKANGGKGDSLNFGINAAQYDY
FLCMDADSMLQEDSLKRIAKTVAKDPSVIAVGGMVKVSQGVALAAGRIVDYHLPRQLISCAQAIEYDCAFFGARVLLDQA
AGNLIISGAFGLFKKELAIAVDGYDTGTLGEDMELVMKLHFFCQNNKIPYRIGYETDAICWSQAPSRLKDLCQQRRRWFL
GLYQCLKKYLQFFVSGRFKVTVLISYVLYLLFELLSPFIEAFGVTIIVLSLLCHQLNTTFFVSLMLLYICYCVLIALTSF
LNRVYSQNNSSITFFDMVWVIYAAIYQCLVLHTVLTLVKVSSFIGYQRKKNAWGQITRDKHKVA
>Mature_464_residues
MQLLFLVLKYSQYLFLAFLIAYASFLLLSLIVGAIHLYERRQQVPLGGDVSLPPVSILVPAYNEELTIIETVESLLMLDY
DCYEIIVIDDGSTDHTAKLLKEQFHLEAVTCSINQRIPTRPLKAVSQKQLKHVKLTLLEKANGGKGDSLNFGINAAQYDY
FLCMDADSMLQEDSLKRIAKTVAKDPSVIAVGGMVKVSQGVALAAGRIVDYHLPRQLISCAQAIEYDCAFFGARVLLDQA
AGNLIISGAFGLFKKELAIAVDGYDTGTLGEDMELVMKLHFFCQNNKIPYRIGYETDAICWSQAPSRLKDLCQQRRRWFL
GLYQCLKKYLQFFVSGRFKVTVLISYVLYLLFELLSPFIEAFGVTIIVLSLLCHQLNTTFFVSLMLLYICYCVLIALTSF
LNRVYSQNNSSITFFDMVWVIYAAIYQCLVLHTVLTLVKVSSFIGYQRKKNAWGQITRDKHKVA

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=456, Percent_Identity=25.4385964912281, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 52403; Mature: 52403

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLFLVLKYSQYLFLAFLIAYASFLLLSLIVGAIHLYERRQQVPLGGDVSLPPVSILVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
AYNEELTIIETVESLLMLDYDCYEIIVIDDGSTDHTAKLLKEQFHLEAVTCSINQRIPTR
CCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCC
PLKAVSQKQLKHVKLTLLEKANGGKGDSLNFGINAAQYDYFLCMDADSMLQEDSLKRIAK
HHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHH
TVAKDPSVIAVGGMVKVSQGVALAAGRIVDYHLPRQLISCAQAIEYDCAFFGARVLLDQA
HHCCCCCEEEECCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNLIISGAFGLFKKELAIAVDGYDTGTLGEDMELVMKLHFFCQNNKIPYRIGYETDAIC
CCCEEEECHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHH
WSQAPSRLKDLCQQRRRWFLGLYQCLKKYLQFFVSGRFKVTVLISYVLYLLFELLSPFIE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AFGVTIIVLSLLCHQLNTTFFVSLMLLYICYCVLIALTSFLNRVYSQNNSSITFFDMVWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
IYAAIYQCLVLHTVLTLVKVSSFIGYQRKKNAWGQITRDKHKVA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MQLLFLVLKYSQYLFLAFLIAYASFLLLSLIVGAIHLYERRQQVPLGGDVSLPPVSILVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
AYNEELTIIETVESLLMLDYDCYEIIVIDDGSTDHTAKLLKEQFHLEAVTCSINQRIPTR
CCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCC
PLKAVSQKQLKHVKLTLLEKANGGKGDSLNFGINAAQYDYFLCMDADSMLQEDSLKRIAK
HHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHH
TVAKDPSVIAVGGMVKVSQGVALAAGRIVDYHLPRQLISCAQAIEYDCAFFGARVLLDQA
HHCCCCCEEEECCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNLIISGAFGLFKKELAIAVDGYDTGTLGEDMELVMKLHFFCQNNKIPYRIGYETDAIC
CCCEEEECHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHH
WSQAPSRLKDLCQQRRRWFLGLYQCLKKYLQFFVSGRFKVTVLISYVLYLLFELLSPFIE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AFGVTIIVLSLLCHQLNTTFFVSLMLLYICYCVLIALTSFLNRVYSQNNSSITFFDMVWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
IYAAIYQCLVLHTVLTLVKVSSFIGYQRKKNAWGQITRDKHKVA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]