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The map label for this gene is ligA [H]

Identifier: 195977913

GI number: 195977913

Start: 813022

End: 814980

Strand: Direct

Name: ligA [H]

Synonym: Sez_0784

Alternate gene names: 195977913

Gene position: 813022-814980 (Clockwise)

Preceding gene: 195977912

Following gene: 195977914

Centisome position: 40.17

GC content: 46.35

Gene sequence:

>1959_bases
ATGAAAAAACGCATAAGAGAATTGACTGATCTGTTAAATCAATACCGTCAGGAGTATTACACCAATGACGCTCCTTCTGT
ATCTGATAGTGAGTATGATAAGCTTTATCGTGAGCTTGTAGAGCTGGAGCAGACCTATCCAGCCTATATCCTAAAGGACA
GCCCCACACAGCTGGTGGGCGGTACGATTTTGACAGGATTTCAGAAATATCAGCACCAGTATCCGCTCTTTAGTTTGCAG
GATGCTTTTTCACGTGAAGAGCTTAACGCCTTTGATCAGCGGATCAAATCAGCCTTTCCTGAGGCTGAATACCTTGCTGA
GCTAAAAATTGATGGCTTATCCATTTCATTGGTTTATGAGGCTGGTATTTTAAAAGTTGGAGCGACGCGTGGCGATGGGA
CTATTGGCGAGAATATCACTGAAAACATTAAGAATATTAAGGATATTCCCAAACGGCTAAGTCAAGCACTTGATGTGACG
ATACGAGGAGAGGCCTATATGTCGCGCAAGGCCTTTAAGACGATCAATGAAGAGCGTCAGGAAAATGGTGAGCCAGAATT
TGCCAATCCAAGAAATGCAGCGGCAGGAACGTTGCGGCAGCTGGATACTAGGATTGTTGCCAAGCGACAGCTAGCGACCT
TTTTGTATCAGGAGGTAGGCTTAGAGGCTGCTGATAGCCAGCAAAGGACCTTGGAGCGCTTGGCTGATTTAGGCTTTTCG
GTCAATAGCCACTATCTGCTGTCCTCATCGATGAATGACATTTGGGACTTTATCCAGAGCATTGAAGCCACTAGAGAAGA
GCTACCCTATGAGATTGATGGTGTGGTGGTCAAGGTTAATCAGTTGGCGATTCAAGAGGAGCTTGGCTTTACCGTCAAGG
CACCACGCTGGGCTATTGCTTATAAGTTTCCAGCAGAAGAAAAGGAAGCTGAGATTGTATCTGTGGATTGGACGGTTGGC
CGAACAGGTGTGGTCACACCAACAGCTAATTTGACCCCTGTGCAGCTGGCAGGCACCACTGTCAGTCGAGCGACCTTGCA
TAATGTTGATTATATCGCAGAAAAGGATATTCGCATTGGGGATACGGTGGTGGTGTATAAGGCTGGAGATATTATTCCGG
CTGTCTTACGAGTGGTTGAGGCAAAGCGCAGCAATCAGACTCCAATGCCTATTCCTACAGCCTGTCCTTCTTGTCAGAGT
CAGCTAGTGCATTTTGAGGACGAGGTAGCTCTGCGCTGTATTAATCCTTTGTGCCCAAGCCTTATTCAGCGGAGCTTGGA
GCATTTTGCCAGTCGTCAGGCGATGAATATCGCTGGTCTTGGTCCAGCGGTTGTTGAAAAGCTTTATTCTGCTGGCCTGG
TGCATGATGTGACAGATATTTACCGACTAAGCTTGGAGGATTTGCTGATGCTAGATGGTATCAAGGAAAAGTCGGCTGAA
AAGCTCTTGGCAGCTATTGAACAATCAAAGGCAAACTCAGCTGAAAAGCTCTTGTTTGGACTTGGTATTCGTCACATTGG
AGCAAAAGCCAGTCGCTTGATATTAGAAACCTATGGTGATTTGGAGACCTTGTTAAGCGTGACTGCTGAGGAGCTAGCGC
AAATTGATGGACTTGGTCTGGTCATTGGGCAATCGCTGGTGCAGTATTTTCAGCAGGATCAAGCAACTCAGCTCTTAGCT
GAATTAAAGTCGGCAGGTGTTAATCTGGCTTATTTGGGCCAAAGACCTGATCGGCAGGCTGAGCTTTTTGGACTCACAGT
AGTATTGACAGGAAAATTAGAAAAACTCAACCGTACTCAAGCCAAGGAAAAGCTAGAGCAGCTTGGTGCTAAGGTAACAG
GAAGCGTTTCTAAAAAAACAGATTTAGTTGTTGCTGGCAGCGAGGCAGGCTCTAAGCTGGCTAAGGCCCAGCAGCTGGGT
ATCAGAATTGAAGATGAGGATTGGTTGCTCAATCTTTAG

Upstream 100 bases:

>100_bases
ATAATAGTAACATGTAGCCTTCCGTTTTTGGGAGGCTTTTTATGATTTTCTTCTAGCTAGGCAATAGCTTATTTGCTATG
TTTTGTGATAAAATAAGACT

Downstream 100 bases:

>100_bases
TGAGGTAATAACATGAAAAAACAAATGAGGGCTCGTCTGATTTACAATCCAACCTCTGGTCAAGAAATCATGAAAAAAAA
CGTTGCAGAGGTGCTGGATA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKKRIRELTDLLNQYRQEYYTNDAPSVSDSEYDKLYRELVELEQTYPAYILKDSPTQLVGGTILTGFQKYQHQYPLFSLQ
DAFSREELNAFDQRIKSAFPEAEYLAELKIDGLSISLVYEAGILKVGATRGDGTIGENITENIKNIKDIPKRLSQALDVT
IRGEAYMSRKAFKTINEERQENGEPEFANPRNAAAGTLRQLDTRIVAKRQLATFLYQEVGLEAADSQQRTLERLADLGFS
VNSHYLLSSSMNDIWDFIQSIEATREELPYEIDGVVVKVNQLAIQEELGFTVKAPRWAIAYKFPAEEKEAEIVSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLRVVEAKRSNQTPMPIPTACPSCQS
QLVHFEDEVALRCINPLCPSLIQRSLEHFASRQAMNIAGLGPAVVEKLYSAGLVHDVTDIYRLSLEDLLMLDGIKEKSAE
KLLAAIEQSKANSAEKLLFGLGIRHIGAKASRLILETYGDLETLLSVTAEELAQIDGLGLVIGQSLVQYFQQDQATQLLA
ELKSAGVNLAYLGQRPDRQAELFGLTVVLTGKLEKLNRTQAKEKLEQLGAKVTGSVSKKTDLVVAGSEAGSKLAKAQQLG
IRIEDEDWLLNL

Sequences:

>Translated_652_residues
MKKRIRELTDLLNQYRQEYYTNDAPSVSDSEYDKLYRELVELEQTYPAYILKDSPTQLVGGTILTGFQKYQHQYPLFSLQ
DAFSREELNAFDQRIKSAFPEAEYLAELKIDGLSISLVYEAGILKVGATRGDGTIGENITENIKNIKDIPKRLSQALDVT
IRGEAYMSRKAFKTINEERQENGEPEFANPRNAAAGTLRQLDTRIVAKRQLATFLYQEVGLEAADSQQRTLERLADLGFS
VNSHYLLSSSMNDIWDFIQSIEATREELPYEIDGVVVKVNQLAIQEELGFTVKAPRWAIAYKFPAEEKEAEIVSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLRVVEAKRSNQTPMPIPTACPSCQS
QLVHFEDEVALRCINPLCPSLIQRSLEHFASRQAMNIAGLGPAVVEKLYSAGLVHDVTDIYRLSLEDLLMLDGIKEKSAE
KLLAAIEQSKANSAEKLLFGLGIRHIGAKASRLILETYGDLETLLSVTAEELAQIDGLGLVIGQSLVQYFQQDQATQLLA
ELKSAGVNLAYLGQRPDRQAELFGLTVVLTGKLEKLNRTQAKEKLEQLGAKVTGSVSKKTDLVVAGSEAGSKLAKAQQLG
IRIEDEDWLLNL
>Mature_652_residues
MKKRIRELTDLLNQYRQEYYTNDAPSVSDSEYDKLYRELVELEQTYPAYILKDSPTQLVGGTILTGFQKYQHQYPLFSLQ
DAFSREELNAFDQRIKSAFPEAEYLAELKIDGLSISLVYEAGILKVGATRGDGTIGENITENIKNIKDIPKRLSQALDVT
IRGEAYMSRKAFKTINEERQENGEPEFANPRNAAAGTLRQLDTRIVAKRQLATFLYQEVGLEAADSQQRTLERLADLGFS
VNSHYLLSSSMNDIWDFIQSIEATREELPYEIDGVVVKVNQLAIQEELGFTVKAPRWAIAYKFPAEEKEAEIVSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLRVVEAKRSNQTPMPIPTACPSCQS
QLVHFEDEVALRCINPLCPSLIQRSLEHFASRQAMNIAGLGPAVVEKLYSAGLVHDVTDIYRLSLEDLLMLDGIKEKSAE
KLLAAIEQSKANSAEKLLFGLGIRHIGAKASRLILETYGDLETLLSVTAEELAQIDGLGLVIGQSLVQYFQQDQATQLLA
ELKSAGVNLAYLGQRPDRQAELFGLTVVLTGKLEKLNRTQAKEKLEQLGAKVTGSVSKKTDLVVAGSEAGSKLAKAQQLG
IRIEDEDWLLNL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=72, Percent_Identity=54.1666666666667, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1788750, Length=665, Percent_Identity=41.3533834586466, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082305, Length=570, Percent_Identity=22.8070175438596, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 72298; Mature: 72298

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIRELTDLLNQYRQEYYTNDAPSVSDSEYDKLYRELVELEQTYPAYILKDSPTQLVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHH
GTILTGFQKYQHQYPLFSLQDAFSREELNAFDQRIKSAFPEAEYLAELKIDGLSISLVYE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCEEEEEEEE
AGILKVGATRGDGTIGENITENIKNIKDIPKRLSQALDVTIRGEAYMSRKAFKTINEERQ
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHH
ENGEPEFANPRNAAAGTLRQLDTRIVAKRQLATFLYQEVGLEAADSQQRTLERLADLGFS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
VNSHYLLSSSMNDIWDFIQSIEATREELPYEIDGVVVKVNQLAIQEELGFTVKAPRWAIA
CCCCEEECCCHHHHHHHHHHHHHHHHHCCEEECCEEEEHHHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEIVSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCCCC
DTVVVYKAGDIIPAVLRVVEAKRSNQTPMPIPTACPSCQSQLVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
LIQRSLEHFASRQAMNIAGLGPAVVEKLYSAGLVHDVTDIYRLSLEDLLMLDGIKEKSAE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEQSKANSAEKLLFGLGIRHIGAKASRLILETYGDLETLLSVTAEELAQIDGLGL
HHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHH
VIGQSLVQYFQQDQATQLLAELKSAGVNLAYLGQRPDRQAELFGLTVVLTGKLEKLNRTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEEEECCHHHHHHHH
AKEKLEQLGAKVTGSVSKKTDLVVAGSEAGSKLAKAQQLGIRIEDEDWLLNL
HHHHHHHHCCHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCEECCCCCEECC
>Mature Secondary Structure
MKKRIRELTDLLNQYRQEYYTNDAPSVSDSEYDKLYRELVELEQTYPAYILKDSPTQLVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHH
GTILTGFQKYQHQYPLFSLQDAFSREELNAFDQRIKSAFPEAEYLAELKIDGLSISLVYE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCEEEEEEEE
AGILKVGATRGDGTIGENITENIKNIKDIPKRLSQALDVTIRGEAYMSRKAFKTINEERQ
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHH
ENGEPEFANPRNAAAGTLRQLDTRIVAKRQLATFLYQEVGLEAADSQQRTLERLADLGFS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
VNSHYLLSSSMNDIWDFIQSIEATREELPYEIDGVVVKVNQLAIQEELGFTVKAPRWAIA
CCCCEEECCCHHHHHHHHHHHHHHHHHCCEEECCEEEEHHHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEIVSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCCCC
DTVVVYKAGDIIPAVLRVVEAKRSNQTPMPIPTACPSCQSQLVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
LIQRSLEHFASRQAMNIAGLGPAVVEKLYSAGLVHDVTDIYRLSLEDLLMLDGIKEKSAE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEQSKANSAEKLLFGLGIRHIGAKASRLILETYGDLETLLSVTAEELAQIDGLGL
HHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHH
VIGQSLVQYFQQDQATQLLAELKSAGVNLAYLGQRPDRQAELFGLTVVLTGKLEKLNRTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEEEECCHHHHHHHH
AKEKLEQLGAKVTGSVSKKTDLVVAGSEAGSKLAKAQQLGIRIEDEDWLLNL
HHHHHHHHCCHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA