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The map label for this gene is manZ [H]

Identifier: 195977876

GI number: 195977876

Start: 777067

End: 777885

Strand: Direct

Name: manZ [H]

Synonym: Sez_0744

Alternate gene names: 195977876

Gene position: 777067-777885 (Clockwise)

Preceding gene: 195977875

Following gene: 195977877

Centisome position: 38.39

GC content: 41.27

Gene sequence:

>819_bases
ATGACGAAATCTAATTATAAATTAACCAAACAGGATTTTAATCAAATCAATAAGCGCAGCCTGTTCACATTCCAATGGGG
CTGGAACTACGAGCGTATGCAGGGCTCTAGCTATCTTTACATGATTTTGCCACAGCTTCGTAAGATTTATGGCGATGGAA
CTCCAGAGCTAAAAGAAATGATGAAGACGCACACGCAGTTTTTTAACACATCAAATTTCTTTCATACGATTATCACAGGA
ATTGACTTGGCCTTGGAGGAAAATGAAGGCCTTGCCTCAAAAGATGCTGTTAATGGGATCAAAACAGGCTTAATGGGGCC
ATTTGCTCCAATTGGTGATTCGGTCTTTGGCTCCTTAGTACCAGCTATTATGGGCTCTATAGCCGCAGGTCTTGCTAAAG
AGGGAGTCTGGTCAGGGGTTACTGGTGTTTTGATGTGGGTCTGTACTCAGATGATCATCAATGTGTTTCGCTGGAAGCAG
CTTGAAATTGCTTATAAAGAAGGTACAAAGCTCGTCACCACCATGCGTGATAAGCTAACCGCTGTAGTAGATGCAGCCTC
AGTCATGGGAGTATTCATGGTAGGGGCCTTAATTGCAACAATGATTAATTTTCAGTTTACCGCAGCACCCAAAATTGGCG
AGAAGGTTATCAATATTCAAGATTTAATTGATACCATTTTTCCACGTCTAGTTCCAGCCTTATTTACCAGAGGTATCTTC
TGGTTGCTAGGGCGTAAGGGCATGTCACCAACTAAGGTAATTGTTGTGATTATTCTATTTGCCTTAGTGATGTCTTATTT
CAATATTTTAGGAGTATAG

Upstream 100 bases:

>100_bases
ATTATTGGACTTGCCTTGGCAACCCTCCATTATAAAAATGGTCAACAAGCACCTGTAGCTGAACAAAAACCTGGGGTATC
AGAAAGTGGGGAAATTGAAG

Downstream 100 bases:

>100_bases
CTGATGGCACAAACCTTAATTCTCATTAGTCATGGAGCCTTTTGTCGAGAGCTAAAAAAGAGTACAGAAATGATTATGGG
GCCACAGGACAATATTCTAA

Product: PTS system mannose/fructose permease IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTKSNYKLTKQDFNQINKRSLFTFQWGWNYERMQGSSYLYMILPQLRKIYGDGTPELKEMMKTHTQFFNTSNFFHTIITG
IDLALEENEGLASKDAVNGIKTGLMGPFAPIGDSVFGSLVPAIMGSIAAGLAKEGVWSGVTGVLMWVCTQMIINVFRWKQ
LEIAYKEGTKLVTTMRDKLTAVVDAASVMGVFMVGALIATMINFQFTAAPKIGEKVINIQDLIDTIFPRLVPALFTRGIF
WLLGRKGMSPTKVIVVIILFALVMSYFNILGV

Sequences:

>Translated_272_residues
MTKSNYKLTKQDFNQINKRSLFTFQWGWNYERMQGSSYLYMILPQLRKIYGDGTPELKEMMKTHTQFFNTSNFFHTIITG
IDLALEENEGLASKDAVNGIKTGLMGPFAPIGDSVFGSLVPAIMGSIAAGLAKEGVWSGVTGVLMWVCTQMIINVFRWKQ
LEIAYKEGTKLVTTMRDKLTAVVDAASVMGVFMVGALIATMINFQFTAAPKIGEKVINIQDLIDTIFPRLVPALFTRGIF
WLLGRKGMSPTKVIVVIILFALVMSYFNILGV
>Mature_271_residues
TKSNYKLTKQDFNQINKRSLFTFQWGWNYERMQGSSYLYMILPQLRKIYGDGTPELKEMMKTHTQFFNTSNFFHTIITGI
DLALEENEGLASKDAVNGIKTGLMGPFAPIGDSVFGSLVPAIMGSIAAGLAKEGVWSGVTGVLMWVCTQMIINVFRWKQL
EIAYKEGTKLVTTMRDKLTAVVDAASVMGVFMVGALIATMINFQFTAAPKIGEKVINIQDLIDTIFPRLVPALFTRGIFW
LLGRKGMSPTKVIVVIILFALVMSYFNILGV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=281, Percent_Identity=31.6725978647687, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789529, Length=266, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30324; Mature: 30193

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSNYKLTKQDFNQINKRSLFTFQWGWNYERMQGSSYLYMILPQLRKIYGDGTPELKEM
CCCCCCEECHHHHHHHHHHCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHH
MKTHTQFFNTSNFFHTIITGIDLALEENEGLASKDAVNGIKTGLMGPFAPIGDSVFGSLV
HHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
PAIMGSIAAGLAKEGVWSGVTGVLMWVCTQMIINVFRWKQLEIAYKEGTKLVTTMRDKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCHHHHHHHHHHH
AVVDAASVMGVFMVGALIATMINFQFTAAPKIGEKVINIQDLIDTIFPRLVPALFTRGIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLLGRKGMSPTKVIVVIILFALVMSYFNILGV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKSNYKLTKQDFNQINKRSLFTFQWGWNYERMQGSSYLYMILPQLRKIYGDGTPELKEM
CCCCCEECHHHHHHHHHHCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHH
MKTHTQFFNTSNFFHTIITGIDLALEENEGLASKDAVNGIKTGLMGPFAPIGDSVFGSLV
HHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
PAIMGSIAAGLAKEGVWSGVTGVLMWVCTQMIINVFRWKQLEIAYKEGTKLVTTMRDKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCHHHHHHHHHHH
AVVDAASVMGVFMVGALIATMINFQFTAAPKIGEKVINIQDLIDTIFPRLVPALFTRGIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLLGRKGMSPTKVIVVIILFALVMSYFNILGV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]