Click here to switch to the map view.

The map label for this gene is kdgA [H]

Identifier: 195977822

GI number: 195977822

Start: 714225

End: 714842

Strand: Direct

Name: kdgA [H]

Synonym: Sez_0688

Alternate gene names: 195977822

Gene position: 714225-714842 (Clockwise)

Preceding gene: 195977821

Following gene: 195977823

Centisome position: 35.28

GC content: 43.85

Gene sequence:

>618_bases
ATGCTAAACCGATTAAAAGAGAATTACTTTTTTGCTGTTATTCGTGGAAAAACAACAGAAGACGCTATTGCTATTGCTAA
GCATGCTATTTTAGGTGGTATTCGCAATATCGAGATTACCTACTCCACTCCAAATGCTTCAGAGGTTATTAGAGCATTGT
CAACTGAGTTTGAGCATGATGAGGCTGTGATTATTGGAGCTGGGACAGTGATGACGCCTGATCTGGCAAGAGAAGCTATT
GCAGCGGGTGCTAGCTTTTTAGTTAGTCCTCATTTTTCAAGAGAGATTGCAGATATTGCTAACCAAGCCTCAAACCTTTA
TTTTCCGGGCTGTGCAACAGCTACCGAGATTGTCACAGCTATGGCTGCTTCTTGTCCGATTATCAAGGTCTTTCCTGGAG
GTGTTGTAGGACCAGGCTTTATTAAGGATATTCATGGTCCTATTCCTGAGGTTGATTTGATGCCATCAGGTGGTGTGTCG
ATTGACAATGTCAAGGAGTGGAAAAATGCAGGTGCTGTTGCTGTTGGGATTGGTTCAGCCTTGGCTAGCCGAGTTGAGGC
TGAGGGCTATGACAGTGTGACTCGTATAGCAAGAGCATTTGTTTCAGCCCTTGATTGA

Upstream 100 bases:

>100_bases
GATACTAGGACCAAGTCCTTTTTTGTTTTTTTCTTGACAAATCATCAGATGATTTGTATAATAAGACTAAGACAACGGTT
TCATAAAAAAGGAGGAGCCT

Downstream 100 bases:

>100_bases
GAAGTAGTAGGTGTGTAAGAGGAGGACGAGATGGCTTTTAATGACGACAATTTTATGCTAAAAAATGAGGCAGCAAAGCG
CCTCTATCAACAAATCAAGG

Product: KHG/KDPG aldolase KdgA

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MLNRLKENYFFAVIRGKTTEDAIAIAKHAILGGIRNIEITYSTPNASEVIRALSTEFEHDEAVIIGAGTVMTPDLAREAI
AAGASFLVSPHFSREIADIANQASNLYFPGCATATEIVTAMAASCPIIKVFPGGVVGPGFIKDIHGPIPEVDLMPSGGVS
IDNVKEWKNAGAVAVGIGSALASRVEAEGYDSVTRIARAFVSALD

Sequences:

>Translated_205_residues
MLNRLKENYFFAVIRGKTTEDAIAIAKHAILGGIRNIEITYSTPNASEVIRALSTEFEHDEAVIIGAGTVMTPDLAREAI
AAGASFLVSPHFSREIADIANQASNLYFPGCATATEIVTAMAASCPIIKVFPGGVVGPGFIKDIHGPIPEVDLMPSGGVS
IDNVKEWKNAGAVAVGIGSALASRVEAEGYDSVTRIARAFVSALD
>Mature_205_residues
MLNRLKENYFFAVIRGKTTEDAIAIAKHAILGGIRNIEITYSTPNASEVIRALSTEFEHDEAVIIGAGTVMTPDLAREAI
AAGASFLVSPHFSREIADIANQASNLYFPGCATATEIVTAMAASCPIIKVFPGGVVGPGFIKDIHGPIPEVDLMPSGGVS
IDNVKEWKNAGAVAVGIGSALASRVEAEGYDSVTRIARAFVSALD

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=195, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788156, Length=185, Percent_Identity=33.5135135135135, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21488; Mature: 21488

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRLKENYFFAVIRGKTTEDAIAIAKHAILGGIRNIEITYSTPNASEVIRALSTEFEHD
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCC
EAVIIGAGTVMTPDLAREAIAAGASFLVSPHFSREIADIANQASNLYFPGCATATEIVTA
CEEEEECCCEECHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHCCEECCCCCHHHHHHHH
MAASCPIIKVFPGGVVGPGFIKDIHGPIPEVDLMPSGGVSIDNVKEWKNAGAVAVGIGSA
HHHCCCEEEECCCCCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHCCCEEEEEHHHH
LASRVEAEGYDSVTRIARAFVSALD
HHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNRLKENYFFAVIRGKTTEDAIAIAKHAILGGIRNIEITYSTPNASEVIRALSTEFEHD
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCC
EAVIIGAGTVMTPDLAREAIAAGASFLVSPHFSREIADIANQASNLYFPGCATATEIVTA
CEEEEECCCEECHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHCCEECCCCCHHHHHHHH
MAASCPIIKVFPGGVVGPGFIKDIHGPIPEVDLMPSGGVSIDNVKEWKNAGAVAVGIGSA
HHHCCCEEEECCCCCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHCCCEEEEEHHHH
LASRVEAEGYDSVTRIARAFVSALD
HHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]