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The map label for this gene is rsuA [H]

Identifier: 195977793

GI number: 195977793

Start: 681909

End: 682625

Strand: Direct

Name: rsuA [H]

Synonym: Sez_0659

Alternate gene names: 195977793

Gene position: 681909-682625 (Clockwise)

Preceding gene: 195977792

Following gene: 195977794

Centisome position: 33.69

GC content: 42.96

Gene sequence:

>717_bases
ATGCGTTTAGATAAATTATTAGAAGTGACAGGCTTTGGTTCTAGAAATCAAGTGAAACAACTCATCAAAGGAAGGTCTGT
CTTGATTGATGGGCAACCAGCTAGAGCTGGTTGTCAGAGTGTTGATTCAGGTCTTCAAGTGATAATGGTAAATGGCAGGC
AAATAAAGGCATTTGCTCATCGCTATTACCTGCTTCATAAGCCAGCAGGGGTTGTGTCAGCTTGCTCTGATAGGAAGCAC
CAAACTGTCATTGATTTGATTCGTAAGGAGGATAGGGTGGCCAATCTTTACCCTATAGGCCGCTTAGATAGAGATACAAC
AGGGCTTGTCCTCTTGACGGATAATGGTCCATTAGGGTTTCGTATGCTTCACCCGAAGCACCATGTGAACAAGACCTACC
TTGTGACTGTCAATGGCTCTTTGGCTGCTGATGCGGTGCCTTTCTTTGAAAATGGTGTTACCTTTCTTGATGGTACTCGC
TGCAAGTCTGCCGCCTTGCGGATTATAAAGGCTGATCAAATGGAGTCACAAGCAGAAGTAACACTCTCTGAAGGAAAATA
CCATCAGATCAAGAAAATGTTTTTAAGCTATGGCTTAAAGGTCACACAGCTCAAGCGAATTGGTTTTGCAGAAATAGCGC
TAGGGGATTTAGCTGAGGGAGCTTATCGTCAGCTAACAGTAAAAGAAAAAAAGCTCATCAAGACCTATTTAACATAA

Upstream 100 bases:

>100_bases
AAGGTTTCTTGCTGACTTTAGCAAAAAATCTGATCAGTTATTAAAAGAGGCTCTTTGGCCTCTTTTTTAGAATTAAGGTA
TATTGTGATAAACTAATGCT

Downstream 100 bases:

>100_bases
AATATTGTTAGATTTGAAGTTGACTGACAGGTTAAGTCCAGTGTTTTTCGGATATATGCTATAGAGAGAACGATAACAAC
TGGAGACCCCTATGCTAACT

Product: ribosomal small subunit pseudouridine synthase A RsuA

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLDKLLEVTGFGSRNQVKQLIKGRSVLIDGQPARAGCQSVDSGLQVIMVNGRQIKAFAHRYYLLHKPAGVVSACSDRKH
QTVIDLIRKEDRVANLYPIGRLDRDTTGLVLLTDNGPLGFRMLHPKHHVNKTYLVTVNGSLAADAVPFFENGVTFLDGTR
CKSAALRIIKADQMESQAEVTLSEGKYHQIKKMFLSYGLKVTQLKRIGFAEIALGDLAEGAYRQLTVKEKKLIKTYLT

Sequences:

>Translated_238_residues
MRLDKLLEVTGFGSRNQVKQLIKGRSVLIDGQPARAGCQSVDSGLQVIMVNGRQIKAFAHRYYLLHKPAGVVSACSDRKH
QTVIDLIRKEDRVANLYPIGRLDRDTTGLVLLTDNGPLGFRMLHPKHHVNKTYLVTVNGSLAADAVPFFENGVTFLDGTR
CKSAALRIIKADQMESQAEVTLSEGKYHQIKKMFLSYGLKVTQLKRIGFAEIALGDLAEGAYRQLTVKEKKLIKTYLT
>Mature_238_residues
MRLDKLLEVTGFGSRNQVKQLIKGRSVLIDGQPARAGCQSVDSGLQVIMVNGRQIKAFAHRYYLLHKPAGVVSACSDRKH
QTVIDLIRKEDRVANLYPIGRLDRDTTGLVLLTDNGPLGFRMLHPKHHVNKTYLVTVNGSLAADAVPFFENGVTFLDGTR
CKSAALRIIKADQMESQAEVTLSEGKYHQIKKMFLSYGLKVTQLKRIGFAEIALGDLAEGAYRQLTVKEKKLIKTYLT

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=37.2294372294372, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1790453, Length=232, Percent_Identity=29.7413793103448, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=28.3950617283951, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=30.3867403314917, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26496; Mature: 26496

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLEVTGFGSRNQVKQLIKGRSVLIDGQPARAGCQSVDSGLQVIMVNGRQIKAFAH
CCHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCEEEEECCCEEEEHHH
RYYLLHKPAGVVSACSDRKHQTVIDLIRKEDRVANLYPIGRLDRDTTGLVLLTDNGPLGF
HEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCCCCE
RMLHPKHHVNKTYLVTVNGSLAADAVPFFENGVTFLDGTRCKSAALRIIKADQMESQAEV
EEECCHHHCCCEEEEEECCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCEE
TLSEGKYHQIKKMFLSYGLKVTQLKRIGFAEIALGDLAEGAYRQLTVKEKKLIKTYLT
EECCCCHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLDKLLEVTGFGSRNQVKQLIKGRSVLIDGQPARAGCQSVDSGLQVIMVNGRQIKAFAH
CCHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCEEEEECCCEEEEHHH
RYYLLHKPAGVVSACSDRKHQTVIDLIRKEDRVANLYPIGRLDRDTTGLVLLTDNGPLGF
HEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCCCCE
RMLHPKHHVNKTYLVTVNGSLAADAVPFFENGVTFLDGTRCKSAALRIIKADQMESQAEV
EEECCHHHCCCEEEEEECCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCEE
TLSEGKYHQIKKMFLSYGLKVTQLKRIGFAEIALGDLAEGAYRQLTVKEKKLIKTYLT
EECCCCHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]