Click here to switch to the map view.

The map label for this gene is recN [H]

Identifier: 195977758

GI number: 195977758

Start: 644871

End: 646571

Strand: Direct

Name: recN [H]

Synonym: Sez_0622

Alternate gene names: 195977758

Gene position: 644871-646571 (Clockwise)

Preceding gene: 195977757

Following gene: 195977759

Centisome position: 31.86

GC content: 44.91

Gene sequence:

>1701_bases
TTGGATGCAGGGATTGGTCTAAAAAATAGAGAGAAGCCGATGTTATTAGAAATTTCCATTAAGAATTTTGCTATCATAGA
GGAAATCTCTTTAAACTTTGAAAACGGTATGACCGTACTGACTGGGGAGACAGGGGCAGGAAAGTCCATTATCATTGATG
CGATGAACATGCTGCTGGGTGCGCGCGCAAGCACTGAGGTGATTCGACATGGTGCTGATAAGGCAGAGATTGAGGGCTTC
TTTTCGGTTGATTCCAATCCTCATTTAGCCGCTGTGCTGGCAGAATCAGGGATTAGCATGGAAGAGGAGCTGATTCTTCG
TCGTGATATTTTTGCTAATGGTCGTAGCGTCAGTCGTATTAACGGTCAAATGGTCACTGTCAGCACCCTAAAAAAACTTG
GGCAGTTTTTAGTGGATATTCATGGTCAGCATGATCAGGAGGAATTGATGCGTCCACAGCTGCACCAGCATATTTTGGAT
AGTTTTGGAGATGAGGTCTTTGACCAGCTCAAGCAGTCCTATCAGCAGATTTTTGATCGCTATAAGGCTTTGCGAAGGCA
GGCTCTTGAGAAGCAAAAAAATGAAAAAGAACATCAGGCGCGCCTTGACCTGCTGGCCTTTCAGATAGGGGAAATTGATG
CAGCAGATTTGGTTAGAGGAGAAGATGACCTGCTTAACCAAGAAAGGCAGCGATTGCTCAATCACAAAAAGATTGCTGAT
ACCTTAACCAGTGCCTACCTTTCCTTGGATAATGAGGACTTTTCCAGTCTCTCACAGGTCCGCTCTAGCATGAATGAGTT
GCTGTCTATTGAGGACCTTGCTCCAGAGTATCAGCTTATGTCAGCTGGTGTGTCAGAGGCTTATTATATTCTTGAGGAGG
TCACAAAGCAATTATCTGATACCATCGACCAGCTTGATTTTGATGCAGGCAGATTGCAGGACATTGAGCTGCGTTTAGAC
AGCATTAATAGCCTGACGAGAAAATATGGCGGTGGTGTGAATGATGTTTTGGACTACTATGCCAATATTACCAAGGAATA
CCAATTATTAAGCGGAGGTGATCGCTCATCTGATGAGCTGGAAAGGGAATTAAAGGCTCTTGAGAAGCAGCTGGTGCTGA
CTGCCTCACAGCTAAGTGATCAGCGTCACGCCCTGGCCTTGGAATTAGAAGCCGACATCAAAGCTGAGCTGAGAGAGCTT
TATATGGAAAAGGCGGACTTTAAGGTCCAGTTTACAAGGTCGAAATTTAACCGAGAAGGACATGAGAGCTTAGAATTTTA
TATCTCAACCAATCCGGGTGAGGGCTTTAAGCCGCTGGTTAAGGTTGCTTCAGGTGGTGAGCTTTCCCGCTTGATGCTTG
CTATCAAGGCTGCCATTTCTAAAAAAGAGGACAAGACAAGTATTGTCTTTGACGAGGTGGATACAGGTGTCTCAGGACGT
GTGGCACAGGCCATTGCTAAAAAAATATATAAAATTGGCCGCTATGGTCAGGTATTAGCGATTTCGCATTTGCCACAGGT
GATTGCTATTGCAGATAACCAGTATTTTATTTCCAAGGAAAGCAAGGAGGAGTCGACAGTGTCTAAGGCTAAGCTGTTGA
CGCAGGAGGAGCGGATCGAGGAAATTGCAAAGATGATTGCAGGAACTGATGTCACAGAGACAGCAAGAAATCAAGCGCGT
GAATTGCTAGGTAAGGACTGA

Upstream 100 bases:

>100_bases
TTTCAGGTCAATTGTTTAGCCTGATTGCAGATGATGATAGCTTGCTGTTGATTGCTAAATCAGAAGCAGACGCTGATCAT
ATCCGTCAGGAATTGTCCCT

Downstream 100 bases:

>100_bases
GTATCATAGCAAAAGCAGAACAGCAGTCTTAGAGCAAGCTTGCCTGCTGCACTTATCGCTACAGGTTCAGAATGAGCCAC
CTGAAAGGCAAGCAAGAAGG

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MDAGIGLKNREKPMLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMLLGARASTEVIRHGADKAEIEGF
FSVDSNPHLAAVLAESGISMEEELILRRDIFANGRSVSRINGQMVTVSTLKKLGQFLVDIHGQHDQEELMRPQLHQHILD
SFGDEVFDQLKQSYQQIFDRYKALRRQALEKQKNEKEHQARLDLLAFQIGEIDAADLVRGEDDLLNQERQRLLNHKKIAD
TLTSAYLSLDNEDFSSLSQVRSSMNELLSIEDLAPEYQLMSAGVSEAYYILEEVTKQLSDTIDQLDFDAGRLQDIELRLD
SINSLTRKYGGGVNDVLDYYANITKEYQLLSGGDRSSDELERELKALEKQLVLTASQLSDQRHALALELEADIKAELREL
YMEKADFKVQFTRSKFNREGHESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKEDKTSIVFDEVDTGVSGR
VAQAIAKKIYKIGRYGQVLAISHLPQVIAIADNQYFISKESKEESTVSKAKLLTQEERIEEIAKMIAGTDVTETARNQAR
ELLGKD

Sequences:

>Translated_566_residues
MDAGIGLKNREKPMLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMLLGARASTEVIRHGADKAEIEGF
FSVDSNPHLAAVLAESGISMEEELILRRDIFANGRSVSRINGQMVTVSTLKKLGQFLVDIHGQHDQEELMRPQLHQHILD
SFGDEVFDQLKQSYQQIFDRYKALRRQALEKQKNEKEHQARLDLLAFQIGEIDAADLVRGEDDLLNQERQRLLNHKKIAD
TLTSAYLSLDNEDFSSLSQVRSSMNELLSIEDLAPEYQLMSAGVSEAYYILEEVTKQLSDTIDQLDFDAGRLQDIELRLD
SINSLTRKYGGGVNDVLDYYANITKEYQLLSGGDRSSDELERELKALEKQLVLTASQLSDQRHALALELEADIKAELREL
YMEKADFKVQFTRSKFNREGHESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKEDKTSIVFDEVDTGVSGR
VAQAIAKKIYKIGRYGQVLAISHLPQVIAIADNQYFISKESKEESTVSKAKLLTQEERIEEIAKMIAGTDVTETARNQAR
ELLGKD
>Mature_566_residues
MDAGIGLKNREKPMLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMLLGARASTEVIRHGADKAEIEGF
FSVDSNPHLAAVLAESGISMEEELILRRDIFANGRSVSRINGQMVTVSTLKKLGQFLVDIHGQHDQEELMRPQLHQHILD
SFGDEVFDQLKQSYQQIFDRYKALRRQALEKQKNEKEHQARLDLLAFQIGEIDAADLVRGEDDLLNQERQRLLNHKKIAD
TLTSAYLSLDNEDFSSLSQVRSSMNELLSIEDLAPEYQLMSAGVSEAYYILEEVTKQLSDTIDQLDFDAGRLQDIELRLD
SINSLTRKYGGGVNDVLDYYANITKEYQLLSGGDRSSDELERELKALEKQLVLTASQLSDQRHALALELEADIKAELREL
YMEKADFKVQFTRSKFNREGHESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKEDKTSIVFDEVDTGVSGR
VAQAIAKKIYKIGRYGQVLAISHLPQVIAIADNQYFISKESKEESTVSKAKLLTQEERIEEIAKMIAGTDVTETARNQAR
ELLGKD

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=561, Percent_Identity=34.2245989304813, Blast_Score=314, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 63488; Mature: 63488

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAGIGLKNREKPMLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMLLG
CCCCCCCCCCCCCEEEEEECCCEEEHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHC
ARASTEVIRHGADKAEIEGFFSVDSNPHLAAVLAESGISMEEELILRRDIFANGRSVSRI
CCHHHHHHHCCCCHHHCCEEEECCCCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCEEEC
NGQMVTVSTLKKLGQFLVDIHGQHDQEELMRPQLHQHILDSFGDEVFDQLKQSYQQIFDR
CCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKALRRQALEKQKNEKEHQARLDLLAFQIGEIDAADLVRGEDDLLNQERQRLLNHKKIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
TLTSAYLSLDNEDFSSLSQVRSSMNELLSIEDLAPEYQLMSAGVSEAYYILEEVTKQLSD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHH
TIDQLDFDAGRLQDIELRLDSINSLTRKYGGGVNDVLDYYANITKEYQLLSGGDRSSDEL
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ERELKALEKQLVLTASQLSDQRHALALELEADIKAELRELYMEKADFKVQFTRSKFNREG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEHHHCCCCC
HESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKEDKTSIVFDEVDTGVSGR
CCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCHH
VAQAIAKKIYKIGRYGQVLAISHLPQVIAIADNQYFISKESKEESTVSKAKLLTQEERIE
HHHHHHHHHHHHCCCCCEEEHHHCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHH
EIAKMIAGTDVTETARNQARELLGKD
HHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAGIGLKNREKPMLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMLLG
CCCCCCCCCCCCCEEEEEECCCEEEHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHC
ARASTEVIRHGADKAEIEGFFSVDSNPHLAAVLAESGISMEEELILRRDIFANGRSVSRI
CCHHHHHHHCCCCHHHCCEEEECCCCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCEEEC
NGQMVTVSTLKKLGQFLVDIHGQHDQEELMRPQLHQHILDSFGDEVFDQLKQSYQQIFDR
CCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKALRRQALEKQKNEKEHQARLDLLAFQIGEIDAADLVRGEDDLLNQERQRLLNHKKIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
TLTSAYLSLDNEDFSSLSQVRSSMNELLSIEDLAPEYQLMSAGVSEAYYILEEVTKQLSD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHH
TIDQLDFDAGRLQDIELRLDSINSLTRKYGGGVNDVLDYYANITKEYQLLSGGDRSSDEL
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ERELKALEKQLVLTASQLSDQRHALALELEADIKAELRELYMEKADFKVQFTRSKFNREG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEHHHCCCCC
HESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKEDKTSIVFDEVDTGVSGR
CCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCHH
VAQAIAKKIYKIGRYGQVLAISHLPQVIAIADNQYFISKESKEESTVSKAKLLTQEERIE
HHHHHHHHHHHHCCCCCEEEHHHCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHH
EIAKMIAGTDVTETARNQARELLGKD
HHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]