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The map label for this gene is folD [H]

Identifier: 195977750

GI number: 195977750

Start: 639224

End: 640078

Strand: Direct

Name: folD [H]

Synonym: Sez_0614

Alternate gene names: 195977750

Gene position: 639224-640078 (Clockwise)

Preceding gene: 195977749

Following gene: 195977751

Centisome position: 31.58

GC content: 45.61

Gene sequence:

>855_bases
ATGGTACAGATTATTGATGGGAAGGCCCTAGCACAGAAAATGCAGGCAGCATTGGCTAAGAAGGTAGAGAGCTTAAAGGC
AGAAAAAGGAATTGTTCCAGGCCTTGTGGTCATTCTGGTAGGAGACAACCCTGCTAGTCAGGTTTATGTCCGTAATAAGG
AACGCGCTGCCTTAGCAGCAGGTTTTAAAAGTGAAACCGTTCGCTTGTCTGATTCAGTCTGTCAGGAAGAGTTGATAGAG
CTCATTGAGCAATATAATCAAGATGATACGATTCATGGTATTTTAGTGCAGCTTCCTCTTCCGCACCATATTAATGACAA
AAGCATTATCCTTGCTATTGATCCTAAAAAGGACGTCGATGGCTTTCACCCTATGAATACAGGTCATCTGTGGTCAGGTC
GTCCGAATATGGTCCCTTGTACTCCGGCGGGGATTATGGAGATGTTTCGTGACTATGGTATCGAGCTTGAAGGGAAAAAT
GCTGTTATTGTCGGGCGCTCAAATATTGTTGGTAAGCCTATGGCTCAGCTCCTGTTGGATAAAAATGCGACGGTAACGCT
TACCCATTCGCGCACGCGCCAGCTGGCAGCTATTTGTCGTCGTGCTGATATCTTGATTGTTGCTATTGGTCAGGGGCATT
TTATCACAAAGGACTTTGTTAAAGAGGGTGCTGTTGTTATAGATGTCGGTATGAATCGTGATGTCAATGGGCGATTGATT
GGAGACGTCGCCTTTGATGAGGTGTCAGAGCTGGCTAGCATGATTACTCCTGTTCCGGGTGGTGTTGGACCGATGACCAT
TACCATGCTTTTGGAGCAAACTTATCAAGCAGCTCTAAGAAGGGTGAGTCAATGA

Upstream 100 bases:

>100_bases
CTGCAACAGAAAATCAGCTAATCAATAAGCAAAAAGGAAAAGGAAGTGACAGTGCTTCCTTTTTTCGTTATAATAGTTAA
AAAATTAGCAAGGAGTGCAT

Downstream 100 bases:

>100_bases
TTATAGATCAATCGTTTGCAGCAAGGGTTATTGTGGAGCGGCAAGCTGACTCCCATAAAGGGAGCTATGGCAGAGTGCTG
CTAGTGGGTGGTCTATATCC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MVQIIDGKALAQKMQAALAKKVESLKAEKGIVPGLVVILVGDNPASQVYVRNKERAALAAGFKSETVRLSDSVCQEELIE
LIEQYNQDDTIHGILVQLPLPHHINDKSIILAIDPKKDVDGFHPMNTGHLWSGRPNMVPCTPAGIMEMFRDYGIELEGKN
AVIVGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLAAICRRADILIVAIGQGHFITKDFVKEGAVVIDVGMNRDVNGRLI
GDVAFDEVSELASMITPVPGGVGPMTITMLLEQTYQAALRRVSQ

Sequences:

>Translated_284_residues
MVQIIDGKALAQKMQAALAKKVESLKAEKGIVPGLVVILVGDNPASQVYVRNKERAALAAGFKSETVRLSDSVCQEELIE
LIEQYNQDDTIHGILVQLPLPHHINDKSIILAIDPKKDVDGFHPMNTGHLWSGRPNMVPCTPAGIMEMFRDYGIELEGKN
AVIVGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLAAICRRADILIVAIGQGHFITKDFVKEGAVVIDVGMNRDVNGRLI
GDVAFDEVSELASMITPVPGGVGPMTITMLLEQTYQAALRRVSQ
>Mature_284_residues
MVQIIDGKALAQKMQAALAKKVESLKAEKGIVPGLVVILVGDNPASQVYVRNKERAALAAGFKSETVRLSDSVCQEELIE
LIEQYNQDDTIHGILVQLPLPHHINDKSIILAIDPKKDVDGFHPMNTGHLWSGRPNMVPCTPAGIMEMFRDYGIELEGKN
AVIVGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLAAICRRADILIVAIGQGHFITKDFVKEGAVVIDVGMNRDVNGRLI
GDVAFDEVSELASMITPVPGGVGPMTITMLLEQTYQAALRRVSQ

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=43.2525951557093, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI222418558, Length=290, Percent_Identity=46.8965517241379, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI94721354, Length=290, Percent_Identity=46.2068965517241, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI36796743, Length=215, Percent_Identity=26.5116279069767, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=49.8194945848375, Blast_Score=277, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI17568735, Length=257, Percent_Identity=43.579766536965, Blast_Score=196, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=44.0972222222222, Blast_Score=246, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=41.4715719063545, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322933, Length=307, Percent_Identity=25.4071661237785, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=46.9594594594595, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=46.9594594594595, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=42.3611111111111, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=42.3611111111111, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=42.3611111111111, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=42.3611111111111, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30890; Mature: 30890

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQIIDGKALAQKMQAALAKKVESLKAEKGIVPGLVVILVGDNPASQVYVRNKERAALAA
CEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHC
GFKSETVRLSDSVCQEELIELIEQYNQDDTIHGILVQLPLPHHINDKSIILAIDPKKDVD
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCC
GFHPMNTGHLWSGRPNMVPCTPAGIMEMFRDYGIELEGKNAVIVGRSNIVGKPMAQLLLD
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHC
KNATVTLTHSRTRQLAAICRRADILIVAIGQGHFITKDFVKEGAVVIDVGMNRDVNGRLI
CCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCEECHHHHHCCCEEEEECCCCCCCCEEE
GDVAFDEVSELASMITPVPGGVGPMTITMLLEQTYQAALRRVSQ
EHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVQIIDGKALAQKMQAALAKKVESLKAEKGIVPGLVVILVGDNPASQVYVRNKERAALAA
CEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHC
GFKSETVRLSDSVCQEELIELIEQYNQDDTIHGILVQLPLPHHINDKSIILAIDPKKDVD
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCC
GFHPMNTGHLWSGRPNMVPCTPAGIMEMFRDYGIELEGKNAVIVGRSNIVGKPMAQLLLD
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHC
KNATVTLTHSRTRQLAAICRRADILIVAIGQGHFITKDFVKEGAVVIDVGMNRDVNGRLI
CCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCEECHHHHHCCCEEEEECCCCCCCCEEE
GDVAFDEVSELASMITPVPGGVGPMTITMLLEQTYQAALRRVSQ
EHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA