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The map label for this gene is murD

Identifier: 195977729

GI number: 195977729

Start: 616445

End: 617803

Strand: Direct

Name: murD

Synonym: Sez_0591

Alternate gene names: 195977729

Gene position: 616445-617803 (Clockwise)

Preceding gene: 195977728

Following gene: 195977730

Centisome position: 30.45

GC content: 45.11

Gene sequence:

>1359_bases
ATGAAGACAATAACGGAATTCCAAAATAAAAAGGTATTGATCCTTGGCCTAGCCAAATCGGGCGAAGCAGCAGCTAGGCT
TTTAGCCCGGTTGGGAGCTATTGTGACAGTTAATGATAGCAAGCCCTTTGAGGAAAATCCCGCAGCACAGGCTCTTCTTG
AGGAGGGCATAAGGGTTATTTGTGGTAGCCACCCCTTGGAGCTGTTAGATGAAGACTTTTATTGTATGGTTAAAAACCCA
GGGATTCGCTATGACAACCCGATGGTGGTATGCGCCTTAGATAAGGCTATTTCTATTCTAACAGAGGTTGAGCTGGCTTA
TTTGGTATCTGAAGCACCTATCATTGGTATTACGGGCTCAAATGGTAAAACCACTACAACCACAATGATTGCTGATGTGC
TAAATGCTGGGAACCAGTCAGCTCTTTTGGCTGGGAATATCGGCTTTCCTGCTTCAGAGGTGGCTCAAGCTGCTACAGCA
AAGGATATACTGGTCATGGAATTGTCTTCCTTTCAGCTGCTGGGGACTGAGCAGTTTCAGCCACATATTGCAGTCATTAC
TAACCTAGTGCCTACTCATCTTGATTATCATGGGAGTTTTGAGGACTATATAGCGGCCAAGTGGCGCATTCAACATCGAA
TGACCGATAAGGATTACCTTGTGTTAAATGCTGATCAAGAGCTGGTAAAATCATTGGCTCAAAAGACCAAAGCAAGTCCT
GTCTTTTTTTCCACCAAAGAAAAGGTTGATGGTGCTTATTTGGCAGATGGGTATCTTTATTTCAAAGAGGAGCAGGTGAT
GTCAGCAGCTGAGCTTGGTCTTCCTGGGAGGCATAACATTGAAAATGCTCTTGCCACCATTGCTGTTGCCAAGCTAAGAG
GCATTTCTAACCAAGTGATTTCAAAGACCCTGTCACACTTTAGTGGTGTGAAGCATCGCCTTCAGTTAGTTGGGACACTG
AATCAGGTGACCTTCTACAATGACAGTAAGTCAACCAATATTTTGGCTTGTCAAAAAGCCTTGTCGGGCTTTGATAATAG
CAGGGTTATCCTGATTGCTGGTGGCTTGGATCGCGGTAATGAATTTGATGAACTGGTGCCAGACCTCGTTGGCCTTAAAA
AGATGATTCTTTTAGGGGAATCCGCAGAGCGTATGAAGCGAGCTGCAGACAAGGCGGGAGTGTCCTATCTTGATGCCAAA
GATGTTGCAGCTGCTACTAAAATAGCCTTTGAGCAGGCGAAGCCTGGTGATATTATCTTGCTGAGTCCAGCTAATGCTAG
CTGGGATATGTATCCAAGCTTTGAAAGCCGCGGCGATGAGTTTTTAGCAGCCTATGATGCGTTAAAAGGAGAGGCCTAA

Upstream 100 bases:

>100_bases
CTTTGTAGTGGCCTTATGCGCTGCTATCTGTAAAATCTTGAAAAAGCAGTTAAATAAGGGTAAAATAGTAGTCAAATAGA
CATAGATAGTGTGGGTAAAA

Downstream 100 bases:

>100_bases
TGACCAAGAAAATCATTTTTACCGGTGGTGGTACAGCTGGTCATGTCACCTTAAACCTTATCCTCATACCGAAGTTTATC
AAGGATGGCTGGGAGGTTCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKTITEFQNKKVLILGLAKSGEAAARLLARLGAIVTVNDSKPFEENPAAQALLEEGIRVICGSHPLELLDEDFYCMVKNP
GIRYDNPMVVCALDKAISILTEVELAYLVSEAPIIGITGSNGKTTTTTMIADVLNAGNQSALLAGNIGFPASEVAQAATA
KDILVMELSSFQLLGTEQFQPHIAVITNLVPTHLDYHGSFEDYIAAKWRIQHRMTDKDYLVLNADQELVKSLAQKTKASP
VFFSTKEKVDGAYLADGYLYFKEEQVMSAAELGLPGRHNIENALATIAVAKLRGISNQVISKTLSHFSGVKHRLQLVGTL
NQVTFYNDSKSTNILACQKALSGFDNSRVILIAGGLDRGNEFDELVPDLVGLKKMILLGESAERMKRAADKAGVSYLDAK
DVAAATKIAFEQAKPGDIILLSPANASWDMYPSFESRGDEFLAAYDALKGEA

Sequences:

>Translated_452_residues
MKTITEFQNKKVLILGLAKSGEAAARLLARLGAIVTVNDSKPFEENPAAQALLEEGIRVICGSHPLELLDEDFYCMVKNP
GIRYDNPMVVCALDKAISILTEVELAYLVSEAPIIGITGSNGKTTTTTMIADVLNAGNQSALLAGNIGFPASEVAQAATA
KDILVMELSSFQLLGTEQFQPHIAVITNLVPTHLDYHGSFEDYIAAKWRIQHRMTDKDYLVLNADQELVKSLAQKTKASP
VFFSTKEKVDGAYLADGYLYFKEEQVMSAAELGLPGRHNIENALATIAVAKLRGISNQVISKTLSHFSGVKHRLQLVGTL
NQVTFYNDSKSTNILACQKALSGFDNSRVILIAGGLDRGNEFDELVPDLVGLKKMILLGESAERMKRAADKAGVSYLDAK
DVAAATKIAFEQAKPGDIILLSPANASWDMYPSFESRGDEFLAAYDALKGEA
>Mature_452_residues
MKTITEFQNKKVLILGLAKSGEAAARLLARLGAIVTVNDSKPFEENPAAQALLEEGIRVICGSHPLELLDEDFYCMVKNP
GIRYDNPMVVCALDKAISILTEVELAYLVSEAPIIGITGSNGKTTTTTMIADVLNAGNQSALLAGNIGFPASEVAQAATA
KDILVMELSSFQLLGTEQFQPHIAVITNLVPTHLDYHGSFEDYIAAKWRIQHRMTDKDYLVLNADQELVKSLAQKTKASP
VFFSTKEKVDGAYLADGYLYFKEEQVMSAAELGLPGRHNIENALATIAVAKLRGISNQVISKTLSHFSGVKHRLQLVGTL
NQVTFYNDSKSTNILACQKALSGFDNSRVILIAGGLDRGNEFDELVPDLVGLKKMILLGESAERMKRAADKAGVSYLDAK
DVAAATKIAFEQAKPGDIILLSPANASWDMYPSFESRGDEFLAAYDALKGEA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=443, Percent_Identity=31.6027088036117, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1786279, Length=303, Percent_Identity=26.4026402640264, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_STREM (B4U1U3)

Other databases:

- EMBL:   CP001129
- RefSeq:   YP_002122973.1
- ProteinModelPortal:   B4U1U3
- EnsemblBacteria:   EBSTRT00000009890
- GeneID:   6761058
- GenomeReviews:   CP001129_GR
- KEGG:   sez:Sez_0591
- GeneTree:   EBGT00050000027197
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48963; Mature: 48963

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTITEFQNKKVLILGLAKSGEAAARLLARLGAIVTVNDSKPFEENPAAQALLEEGIRVI
CCCCHHHCCCEEEEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCEEE
CGSHPLELLDEDFYCMVKNPGIRYDNPMVVCALDKAISILTEVELAYLVSEAPIIGITGS
ECCCCHHHHCCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECC
NGKTTTTTMIADVLNAGNQSALLAGNIGFPASEVAQAATAKDILVMELSSFQLLGTEQFQ
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCEEEECCCCCC
PHIAVITNLVPTHLDYHGSFEDYIAAKWRIQHRMTDKDYLVLNADQELVKSLAQKTKASP
CHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCC
VFFSTKEKVDGAYLADGYLYFKEEQVMSAAELGLPGRHNIENALATIAVAKLRGISNQVI
EEEECHHHCCCEEEECCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHH
SKTLSHFSGVKHRLQLVGTLNQVTFYNDSKSTNILACQKALSGFDNSRVILIAGGLDRGN
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
EFDELVPDLVGLKKMILLGESAERMKRAADKAGVSYLDAKDVAAATKIAFEQAKPGDIIL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCEEE
LSPANASWDMYPSFESRGDEFLAAYDALKGEA
ECCCCCCCCCCCCHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKTITEFQNKKVLILGLAKSGEAAARLLARLGAIVTVNDSKPFEENPAAQALLEEGIRVI
CCCCHHHCCCEEEEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCEEE
CGSHPLELLDEDFYCMVKNPGIRYDNPMVVCALDKAISILTEVELAYLVSEAPIIGITGS
ECCCCHHHHCCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECC
NGKTTTTTMIADVLNAGNQSALLAGNIGFPASEVAQAATAKDILVMELSSFQLLGTEQFQ
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCEEEECCCCCC
PHIAVITNLVPTHLDYHGSFEDYIAAKWRIQHRMTDKDYLVLNADQELVKSLAQKTKASP
CHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCC
VFFSTKEKVDGAYLADGYLYFKEEQVMSAAELGLPGRHNIENALATIAVAKLRGISNQVI
EEEECHHHCCCEEEECCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHH
SKTLSHFSGVKHRLQLVGTLNQVTFYNDSKSTNILACQKALSGFDNSRVILIAGGLDRGN
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
EFDELVPDLVGLKKMILLGESAERMKRAADKAGVSYLDAKDVAAATKIAFEQAKPGDIIL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCEEE
LSPANASWDMYPSFESRGDEFLAAYDALKGEA
ECCCCCCCCCCCCHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA