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The map label for this gene is nusA [H]

Identifier: 195977581

GI number: 195977581

Start: 463910

End: 465064

Strand: Direct

Name: nusA [H]

Synonym: Sez_0440

Alternate gene names: 195977581

Gene position: 463910-465064 (Clockwise)

Preceding gene: 195977580

Following gene: 195977582

Centisome position: 22.92

GC content: 43.81

Gene sequence:

>1155_bases
ATGAGCAAAGAAATGCTAGAAGCCTTCCGTATTTTGGAAGAAGAAAAACACATTGATAAGGCAGATATCATTGAGGCAGT
TACTGAATCACTGAAATCAGCTTATAAACGTCGTTATGGTCAAGCAGAATCCTGCGTCATTGAATTTAATGATAAAACAG
CAGATTTTCAGGTTTTTACGGTTCGTGAGGTTGTTGAAGAGGTATTTGATAGCCGTCTGGAAATTAGCCTGAACGATGCC
CTTGCCATTAGCTCAGCCTATGAGTTAGGAGATAAAATTCGTTTTGAAGAGTCAGTCAGCGAGTTTGGACGTGTGGCTGC
TCAGTCTGCAAAGCAAACCATTATGGAAAGGATGCGTCGTCAGATGCGAGAAATCACCTTCAATGAGTACAAGCAGCATG
AAGGTGAAATCATGACGGGTACGGTTGAGCGTTTTGACCAACGTTTCATTTATGTTAACCTTGGTTCCTTAGAGGCTCAG
CTCTCACATCAGGATCAAATCCCAGGAGAAACCTTCAAATCACATGATCGGATTGATGTCTATGTCTACAAGGTGGAAAA
CAATCCAAAGGGGGTTAACGTCTTTGTTAGTCGTAGTCACCCTGAGTTCATCAAGCGCATTATGGAACAGGAAATTCCAG
AGGTTTTTGATGGGACCGTCGAGATTATGAGTGTTTCTCGTGAGGCGGGTGACCGTACTAAGGTTGCTGTGCGTAGCCAC
AATCCCAATGTTGATGCGATTGGAACGATTGTTGGTCGTGGCGGCAGCAACATCAAAAAGGTTATCAGTAAATTCCATCC
AAAGCGCTATGATGCTAAGACAGGACTTGAGATCCCAGTAGAAGAAAACATTGACGTTATCCAATGGGTAGATGACCCTG
CTGAATTTATCTACAATGCCATTGCACCTGCTGAGGTTGATATGGTTCTCTTTGATGATGACGATCTTAAGCGTGCGACT
GTGGTGGTGCCTGATAATAAGCTATCGCTTGCTATTGGCCGTCGTGGTCAAAACGTTCGTTTGGCAGCTCATTTGACAGG
CTATCGCATTGACATCAAATCAGCCAGTGAATATGAACGCCTTGAAGCAGAAAAAGAAGCAGCAATGACTGAAGCAGTAG
CTACAGAGGAGCTCTTAACCAACGAGGAAGTGTAA

Upstream 100 bases:

>100_bases
TGAGCGATCTGTCTTAGTCGGTTGGCTAAGCCCTAAATGGATTAATTTATAAGATAAGAAAGGATTTTTACCAAGCTTTG
AGAGCAAGGTAGACAACATT

Downstream 100 bases:

>100_bases
GAAAAGAGGTGTTTTATGCCTAAAGTAAAAAAAATACCTTTACGTAAGTCGCTTGTTTCTGGTGAGATCATTGATAAGCG
TGACCTCCTTCGTATTGTCA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSKEMLEAFRILEEEKHIDKADIIEAVTESLKSAYKRRYGQAESCVIEFNDKTADFQVFTVREVVEEVFDSRLEISLNDA
LAISSAYELGDKIRFEESVSEFGRVAAQSAKQTIMERMRRQMREITFNEYKQHEGEIMTGTVERFDQRFIYVNLGSLEAQ
LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
NPNVDAIGTIVGRGGSNIKKVISKFHPKRYDAKTGLEIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDDDLKRAT
VVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYERLEAEKEAAMTEAVATEELLTNEEV

Sequences:

>Translated_384_residues
MSKEMLEAFRILEEEKHIDKADIIEAVTESLKSAYKRRYGQAESCVIEFNDKTADFQVFTVREVVEEVFDSRLEISLNDA
LAISSAYELGDKIRFEESVSEFGRVAAQSAKQTIMERMRRQMREITFNEYKQHEGEIMTGTVERFDQRFIYVNLGSLEAQ
LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
NPNVDAIGTIVGRGGSNIKKVISKFHPKRYDAKTGLEIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDDDLKRAT
VVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYERLEAEKEAAMTEAVATEELLTNEEV
>Mature_383_residues
SKEMLEAFRILEEEKHIDKADIIEAVTESLKSAYKRRYGQAESCVIEFNDKTADFQVFTVREVVEEVFDSRLEISLNDAL
AISSAYELGDKIRFEESVSEFGRVAAQSAKQTIMERMRRQMREITFNEYKQHEGEIMTGTVERFDQRFIYVNLGSLEAQL
SHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSHN
PNVDAIGTIVGRGGSNIKKVISKFHPKRYDAKTGLEIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDDDLKRATV
VVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYERLEAEKEAAMTEAVATEELLTNEEV

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=372, Percent_Identity=32.5268817204301, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 43739; Mature: 43608

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMLEAFRILEEEKHIDKADIIEAVTESLKSAYKRRYGQAESCVIEFNDKTADFQVFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEE
VREVVEEVFDSRLEISLNDALAISSAYELGDKIRFEESVSEFGRVAAQSAKQTIMERMRR
HHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMREITFNEYKQHEGEIMTGTVERFDQRFIYVNLGSLEAQLSHQDQIPGETFKSHDRIDV
HHHHCCHHHHHHCCCCEEEHHHHHHCCEEEEEEECCHHHHHCCCCCCCCCHHCCCCCEEE
YVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECC
NPNVDAIGTIVGRGGSNIKKVISKFHPKRYDAKTGLEIPVEENIDVIQWVDDPAEFIYNA
CCCCCEEEHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHH
IAPAEVDMVLFDDDDLKRATVVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYER
CCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEECCCHHHHH
LEAEKEAAMTEAVATEELLTNEEV
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKEMLEAFRILEEEKHIDKADIIEAVTESLKSAYKRRYGQAESCVIEFNDKTADFQVFT
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEE
VREVVEEVFDSRLEISLNDALAISSAYELGDKIRFEESVSEFGRVAAQSAKQTIMERMRR
HHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMREITFNEYKQHEGEIMTGTVERFDQRFIYVNLGSLEAQLSHQDQIPGETFKSHDRIDV
HHHHCCHHHHHHCCCCEEEHHHHHHCCEEEEEEECCHHHHHCCCCCCCCCHHCCCCCEEE
YVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECC
NPNVDAIGTIVGRGGSNIKKVISKFHPKRYDAKTGLEIPVEENIDVIQWVDDPAEFIYNA
CCCCCEEEHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHH
IAPAEVDMVLFDDDDLKRATVVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYER
CCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEECCCHHHHH
LEAEKEAAMTEAVATEELLTNEEV
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]