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The map label for this gene is trmB [H]

Identifier: 195977578

GI number: 195977578

Start: 461300

End: 461935

Strand: Direct

Name: trmB [H]

Synonym: Sez_0437

Alternate gene names: 195977578

Gene position: 461300-461935 (Clockwise)

Preceding gene: 195977577

Following gene: 195977580

Centisome position: 22.79

GC content: 43.71

Gene sequence:

>636_bases
ATGCGAGTTAGAAAACGAAAGGGTGCGCAGGAGTACTTGGAAAACAATCCTCACTATGTCATTTTAGAGCCGGAGGCTGC
TAAGGGGCGCTGGTGTGAGGTCTTTGGTAATGATCATCCGATCCATATTGAGGTTGGCTCAGGCAAGGGAGCTTTTATCA
CAGGTATGGCGTTGAAAAATCCAGAGATCAACTATATTGGCATTGACATTCAGCTATCAGTGCTAAGCTATGCTTTGGAT
AAGGTCCTAGCTTCTCAGGCTCCTAATATTAGGCTCTTGCGGGTTGATGGGTCAAGCCTGACCAATTATTTCGATGCTGG
TGAGATTGACATGATGTATTTGAATTTTTCAGATCCTTGGCCAAAAAGCAGGCACGAGAAGCGCCGATTAACCTACAAGT
CCTTTTTAGACACCTATAAGCAGATTTTGCCAGAAAATGGTGAGATTCATTTTAAAACAGACAATCGCGGCTTATTTGAG
TATAGCCTAGCAAGCTTTTCACAGTACGGTATGACCCTCAAGCAGGTCTGGCTGGATTTACATGCTAGTGATTACCCAGA
CAATGTCATGACCGAGTATGAAGCTAGATTTGCTAAAAAGGGGCAGGTTATTTACCGCTTAGAAGCAACATTCTAG

Upstream 100 bases:

>100_bases
TGACTCAGATTTTAAGGTGCTTTGATAAGAGAGATATGGAGCATGTCAATCAAGAGATTTACGCTCTTAGAAAATTTAGA
GAAATGTTTAGAAAGAAATA

Downstream 100 bases:

>100_bases
TGGCTATTTTGACAAAAATATGATATACTATTGAGGTTGAGTTGATCAGCCTTTTATCTGGAGAGGTCGCATAGTGGCCG
AGTGCGCACGCTTGGAAAGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRVRKRKGAQEYLENNPHYVILEPEAAKGRWCEVFGNDHPIHIEVGSGKGAFITGMALKNPEINYIGIDIQLSVLSYALD
KVLASQAPNIRLLRVDGSSLTNYFDAGEIDMMYLNFSDPWPKSRHEKRRLTYKSFLDTYKQILPENGEIHFKTDNRGLFE
YSLASFSQYGMTLKQVWLDLHASDYPDNVMTEYEARFAKKGQVIYRLEATF

Sequences:

>Translated_211_residues
MRVRKRKGAQEYLENNPHYVILEPEAAKGRWCEVFGNDHPIHIEVGSGKGAFITGMALKNPEINYIGIDIQLSVLSYALD
KVLASQAPNIRLLRVDGSSLTNYFDAGEIDMMYLNFSDPWPKSRHEKRRLTYKSFLDTYKQILPENGEIHFKTDNRGLFE
YSLASFSQYGMTLKQVWLDLHASDYPDNVMTEYEARFAKKGQVIYRLEATF
>Mature_211_residues
MRVRKRKGAQEYLENNPHYVILEPEAAKGRWCEVFGNDHPIHIEVGSGKGAFITGMALKNPEINYIGIDIQLSVLSYALD
KVLASQAPNIRLLRVDGSSLTNYFDAGEIDMMYLNFSDPWPKSRHEKRRLTYKSFLDTYKQILPENGEIHFKTDNRGLFE
YSLASFSQYGMTLKQVWLDLHASDYPDNVMTEYEARFAKKGQVIYRLEATF

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=165, Percent_Identity=29.0909090909091, Blast_Score=71, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320000, Length=137, Percent_Identity=26.2773722627737, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24356; Mature: 24356

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRKRKGAQEYLENNPHYVILEPEAAKGRWCEVFGNDHPIHIEVGSGKGAFITGMALKN
CCCCHHCCHHHHHHCCCCEEEECCCCCCCCEEEECCCCCEEEEEECCCCCEEEEEEEECC
PEINYIGIDIQLSVLSYALDKVLASQAPNIRLLRVDGSSLTNYFDAGEIDMMYLNFSDPW
CCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCCCCEEEEEEECCCCC
PKSRHEKRRLTYKSFLDTYKQILPENGEIHFKTDNRGLFEYSLASFSQYGMTLKQVWLDL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHCHHHHHHHHHH
HASDYPDNVMTEYEARFAKKGQVIYRLEATF
CCCCCCCHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MRVRKRKGAQEYLENNPHYVILEPEAAKGRWCEVFGNDHPIHIEVGSGKGAFITGMALKN
CCCCHHCCHHHHHHCCCCEEEECCCCCCCCEEEECCCCCEEEEEECCCCCEEEEEEEECC
PEINYIGIDIQLSVLSYALDKVLASQAPNIRLLRVDGSSLTNYFDAGEIDMMYLNFSDPW
CCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCCCCEEEEEEECCCCC
PKSRHEKRRLTYKSFLDTYKQILPENGEIHFKTDNRGLFEYSLASFSQYGMTLKQVWLDL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHCHHHHHHHHHH
HASDYPDNVMTEYEARFAKKGQVIYRLEATF
CCCCCCCHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA