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The map label for this gene is gla [H]

Identifier: 195977468

GI number: 195977468

Start: 341579

End: 342409

Strand: Direct

Name: gla [H]

Synonym: Sez_0323

Alternate gene names: 195977468

Gene position: 341579-342409 (Clockwise)

Preceding gene: 195977467

Following gene: 195977469

Centisome position: 16.88

GC content: 45.97

Gene sequence:

>831_bases
GTGAAATACATCACAGAGTTTATTGCCACTGCATTTTTGATTATTTTGGGAAATGGAGCAGTTGCCAATGTTGACTTAAA
GGGAACAAAGGGTAACCGCTCAGGCTGGCTTGTGATTGCCTTTGGCTATGGCCTTGGTGTGATGATGCCGGCACTTATGT
TTGGTAACGTTTCTGGAAATCACATTAACCCAGCCTTTACCTTTGGCTTGGCAGTTTCAGGCTTGTTCCCTTGGGCACAC
GTTTTACAATATGTTCTTGCTCAATTGCTAGGGGCTATGTTTGGTCAATTAGTGGTTGTTGCAGTGTACAAGCCTTATTT
TGTGCAGACAGATAACCCTAACAATATTTTGGGCTCCTTCTCAACGATTTCAGCAGTTGATGATGGTGCCAAGAGCAGCC
GTAAGGCAGCCTACATTAATGGCTTTTTGAACGAGTTTGTTGGTTCCTTTGTCCTCTTTTTTGGCGCAATGGCCTTAACC
AAGAATTACTTTGGTGTGGAGCTTGTTGGTAAGCTGGTAGAGGCTGGCTATGACCAAACAACAGCTGCAACGCAAATCTC
TCCTTACGTGACAGGCTCACTTGCAGTAGCTCACTTGGGGATTGGTTTCTTGGTGATGACTCTTGTGACCTCTCTTGGTG
GTCCTACTGGTCCAGCGCTCAACCCAGCGCGTGACTTAGGCCCTCGTTTGGTTCACCATTTCTTACCAAAATCAGTCCTT
GGACAAGCCAAGGGGGATTCAAAATGGTGGTACTCATGGGTACCGGTTGCTGCTCCAGTACTGGCAGCAGTAGTAGCAGT
AGCAGCATTCAAATTCCTTTATATCAAGTAA

Upstream 100 bases:

>100_bases
TCTTACAATAGGCTTTACAAGAACTAGTATCCCTGATAAAATGGTATCTGTTATACTTATCATTTGGAGGAAACTAGAGA
ATATGGAAATAACATGGACT

Downstream 100 bases:

>100_bases
AAGAGAAGGCTTAGGCCTTCTTTTTTGTGACAAATGCTTGTCAGCAAGAGCCAGCTGAGTCTGAACTGAGCTAAGCTGGT
GGCCTCCTTATCAGTATTGA

Product: glycerol facilitator-aquaporin Gla

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin; Glyceroaquaporin [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKYITEFIATAFLIILGNGAVANVDLKGTKGNRSGWLVIAFGYGLGVMMPALMFGNVSGNHINPAFTFGLAVSGLFPWAH
VLQYVLAQLLGAMFGQLVVVAVYKPYFVQTDNPNNILGSFSTISAVDDGAKSSRKAAYINGFLNEFVGSFVLFFGAMALT
KNYFGVELVGKLVEAGYDQTTAATQISPYVTGSLAVAHLGIGFLVMTLVTSLGGPTGPALNPARDLGPRLVHHFLPKSVL
GQAKGDSKWWYSWVPVAAPVLAAVVAVAAFKFLYIK

Sequences:

>Translated_276_residues
MKYITEFIATAFLIILGNGAVANVDLKGTKGNRSGWLVIAFGYGLGVMMPALMFGNVSGNHINPAFTFGLAVSGLFPWAH
VLQYVLAQLLGAMFGQLVVVAVYKPYFVQTDNPNNILGSFSTISAVDDGAKSSRKAAYINGFLNEFVGSFVLFFGAMALT
KNYFGVELVGKLVEAGYDQTTAATQISPYVTGSLAVAHLGIGFLVMTLVTSLGGPTGPALNPARDLGPRLVHHFLPKSVL
GQAKGDSKWWYSWVPVAAPVLAAVVAVAAFKFLYIK
>Mature_276_residues
MKYITEFIATAFLIILGNGAVANVDLKGTKGNRSGWLVIAFGYGLGVMMPALMFGNVSGNHINPAFTFGLAVSGLFPWAH
VLQYVLAQLLGAMFGQLVVVAVYKPYFVQTDNPNNILGSFSTISAVDDGAKSSRKAAYINGFLNEFVGSFVLFFGAMALT
KNYFGVELVGKLVEAGYDQTTAATQISPYVTGSLAVAHLGIGFLVMTLVTSLGGPTGPALNPARDLGPRLVHHFLPKSVL
GQAKGDSKWWYSWVPVAAPVLAAVVAVAAFKFLYIK

Specific function: Mixed channel protein that transports both water and glycerol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=281, Percent_Identity=30.2491103202847, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI22538420, Length=280, Percent_Identity=31.4285714285714, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157266307, Length=271, Percent_Identity=28.7822878228782, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1790362, Length=276, Percent_Identity=29.3478260869565, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=275, Percent_Identity=26.5454545454545, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531431, Length=275, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17531429, Length=275, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17544068, Length=278, Percent_Identity=26.2589928057554, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71992966, Length=272, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=225, Percent_Identity=31.1111111111111, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29323; Mature: 29323

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYITEFIATAFLIILGNGAVANVDLKGTKGNRSGWLVIAFGYGLGVMMPALMFGNVSGN
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCC
HINPAFTFGLAVSGLFPWAHVLQYVLAQLLGAMFGQLVVVAVYKPYFVQTDNPNNILGSF
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHCCC
STISAVDDGAKSSRKAAYINGFLNEFVGSFVLFFGAMALTKNYFGVELVGKLVEAGYDQT
HHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
TAATQISPYVTGSLAVAHLGIGFLVMTLVTSLGGPTGPALNPARDLGPRLVHHFLPKSVL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
GQAKGDSKWWYSWVPVAAPVLAAVVAVAAFKFLYIK
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYITEFIATAFLIILGNGAVANVDLKGTKGNRSGWLVIAFGYGLGVMMPALMFGNVSGN
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCC
HINPAFTFGLAVSGLFPWAHVLQYVLAQLLGAMFGQLVVVAVYKPYFVQTDNPNNILGSF
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHCCC
STISAVDDGAKSSRKAAYINGFLNEFVGSFVLFFGAMALTKNYFGVELVGKLVEAGYDQT
HHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
TAATQISPYVTGSLAVAHLGIGFLVMTLVTSLGGPTGPALNPARDLGPRLVHHFLPKSVL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
GQAKGDSKWWYSWVPVAAPVLAAVVAVAAFKFLYIK
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]