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The map label for this gene is saeS [H]

Identifier: 195977457

GI number: 195977457

Start: 328697

End: 329707

Strand: Direct

Name: saeS [H]

Synonym: Sez_0312

Alternate gene names: 195977457

Gene position: 328697-329707 (Clockwise)

Preceding gene: 195977456

Following gene: 195977459

Centisome position: 16.24

GC content: 42.14

Gene sequence:

>1011_bases
ATGTCTATGCTGATTACTATTGCAGGTCTGATTGTTGGGTTAAACCAAATGCTCATTACCATTGAGGATATTTCATATAT
TTTAGTAATAGCTCTAATTGCTTCAGTAGCCGGAGGGATTGTGAATATGATCCTTTTGAGTAATGTTTTTTCATCGCTTA
AAAGGCTAAAGAAAAAGATACAGGCTATTTCTGAGAGAAACTTTGATAGTGGTCAGCTCATCAAGTGTCCTTTGGAGTTT
AAAGATCTGGAGGAAGCCTTTAATCAGATGTCCTCAGAGCTGAAGGTCAGCTTTGAGTCATTGAGTGAGAGTGAGCATGA
GAAATCCATGATGATTGCTCAGCTGTCGCACGATATTAAGACACCCTTGACCTCTATTCAAGCAACTGTAGAGGGGATTT
TAGATGGCGTTATTCCAAGGGAAGAGGAGAGGCACTATCTAAACACCATCTCACGACAGACCAACCGTTTGAATCAATTA
GTTGAAGAATTGCATATGGTCAGCTTAAATGATCAAAAGCAAGACGACAAGCAGCAGCTTCAAATCATCTATCTTGAAAA
GCTATTGATTGATATGCTATCAGAATTTCAATTGACCTTAGAGCAGGAAAATCGCTCCGTTCATATTGAGGTGGCAAAAG
AGGTGGTTAAAATCAACAGCCGATACGATGCTTTGTCTCGGATTATGCTGAATTTAGTCAGCAATGCGCTTAAATATTCA
AAGGCTAATACGGCTTTGACGATTCATGCTTATCAACAGGAGCAGTCCATTCGCATTGATGTTATTGATCAGGGGCAAGG
GATCAAAAAAGAGGACCTGCCTTTGATTTTCAAGCGCCTGTATCGTGTTGAGGCCTCTCGCAATATGAAGACTGGTGGTC
ATGGGCTGGGGCTCTCTATTGCTAGGCAGTTAGCTCGCCAACTAGGTGGGGATATTGCTGTTGAGAGCCAGCTTGGAAAG
GGGAGCTGTTTCTCCTTGCTTTTACCAGCCAGCCCACCATTAGGAGACTAG

Upstream 100 bases:

>100_bases
AGTACCGAGCAAACACCAACCATTAAAACGGTTTGGGGACTAGGCTATAAATTAGAGGAGTAGTATGCGACTAAGAACAT
ACATTGTTATCGGCTATTTA

Downstream 100 bases:

>100_bases
ACCTTTAGGTAATAGGCATATGGTTATAAAGCTAGGACATCACAAAGGCTTATCAGTAAGTCCGGAGCAAGCATCAGCAG
CAAGGCTGGTGCTTGTTTTT

Product: two component system histidine kinase

Products: NA

Alternate protein names: Sensor protein saeS [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSMLITIAGLIVGLNQMLITIEDISYILVIALIASVAGGIVNMILLSNVFSSLKRLKKKIQAISERNFDSGQLIKCPLEF
KDLEEAFNQMSSELKVSFESLSESEHEKSMMIAQLSHDIKTPLTSIQATVEGILDGVIPREEERHYLNTISRQTNRLNQL
VEELHMVSLNDQKQDDKQQLQIIYLEKLLIDMLSEFQLTLEQENRSVHIEVAKEVVKINSRYDALSRIMLNLVSNALKYS
KANTALTIHAYQQEQSIRIDVIDQGQGIKKEDLPLIFKRLYRVEASRNMKTGGHGLGLSIARQLARQLGGDIAVESQLGK
GSCFSLLLPASPPLGD

Sequences:

>Translated_336_residues
MSMLITIAGLIVGLNQMLITIEDISYILVIALIASVAGGIVNMILLSNVFSSLKRLKKKIQAISERNFDSGQLIKCPLEF
KDLEEAFNQMSSELKVSFESLSESEHEKSMMIAQLSHDIKTPLTSIQATVEGILDGVIPREEERHYLNTISRQTNRLNQL
VEELHMVSLNDQKQDDKQQLQIIYLEKLLIDMLSEFQLTLEQENRSVHIEVAKEVVKINSRYDALSRIMLNLVSNALKYS
KANTALTIHAYQQEQSIRIDVIDQGQGIKKEDLPLIFKRLYRVEASRNMKTGGHGLGLSIARQLARQLGGDIAVESQLGK
GSCFSLLLPASPPLGD
>Mature_335_residues
SMLITIAGLIVGLNQMLITIEDISYILVIALIASVAGGIVNMILLSNVFSSLKRLKKKIQAISERNFDSGQLIKCPLEFK
DLEEAFNQMSSELKVSFESLSESEHEKSMMIAQLSHDIKTPLTSIQATVEGILDGVIPREEERHYLNTISRQTNRLNQLV
EELHMVSLNDQKQDDKQQLQIIYLEKLLIDMLSEFQLTLEQENRSVHIEVAKEVVKINSRYDALSRIMLNLVSNALKYSK
ANTALTIHAYQQEQSIRIDVIDQGQGIKKEDLPLIFKRLYRVEASRNMKTGGHGLGLSIARQLARQLGGDIAVESQLGKG
SCFSLLLPASPPLGD

Specific function: Member of the two-component regulatory system saeR/saeS. Probably functions as a membrane-associated protein kinase that upon sensing the appropriate signal, autophosphorylates and in turn activates the cytosolic response regulator saeR [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=25.7950530035336, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786600, Length=237, Percent_Identity=29.535864978903, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994928, Length=258, Percent_Identity=29.0697674418605, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786783, Length=325, Percent_Identity=23.6923076923077, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=27.2340425531915, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790346, Length=277, Percent_Identity=26.3537906137184, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=28.2511210762332, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787894, Length=312, Percent_Identity=26.2820512820513, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=259, Percent_Identity=29.3436293436293, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=27.4261603375527, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=26.2962962962963, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=251, Percent_Identity=23.9043824701195, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=22.6190476190476, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=334, Percent_Identity=23.6526946107784, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790300, Length=252, Percent_Identity=26.5873015873016, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 37630; Mature: 37499

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLITIAGLIVGLNQMLITIEDISYILVIALIASVAGGIVNMILLSNVFSSLKRLKKKI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAISERNFDSGQLIKCPLEFKDLEEAFNQMSSELKVSFESLSESEHEKSMMIAQLSHDIK
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TPLTSIQATVEGILDGVIPREEERHYLNTISRQTNRLNQLVEELHMVSLNDQKQDDKQQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QIIYLEKLLIDMLSEFQLTLEQENRSVHIEVAKEVVKINSRYDALSRIMLNLVSNALKYS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KANTALTIHAYQQEQSIRIDVIDQGQGIKKEDLPLIFKRLYRVEASRNMKTGGHGLGLSI
CCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ARQLARQLGGDIAVESQLGKGSCFSLLLPASPPLGD
HHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SMLITIAGLIVGLNQMLITIEDISYILVIALIASVAGGIVNMILLSNVFSSLKRLKKKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAISERNFDSGQLIKCPLEFKDLEEAFNQMSSELKVSFESLSESEHEKSMMIAQLSHDIK
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TPLTSIQATVEGILDGVIPREEERHYLNTISRQTNRLNQLVEELHMVSLNDQKQDDKQQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QIIYLEKLLIDMLSEFQLTLEQENRSVHIEVAKEVVKINSRYDALSRIMLNLVSNALKYS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KANTALTIHAYQQEQSIRIDVIDQGQGIKKEDLPLIFKRLYRVEASRNMKTGGHGLGLSI
CCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ARQLARQLGGDIAVESQLGKGSCFSLLLPASPPLGD
HHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA