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The map label for this gene is oppB [H]

Identifier: 195977425

GI number: 195977425

Start: 300546

End: 301460

Strand: Direct

Name: oppB [H]

Synonym: Sez_0280

Alternate gene names: 195977425

Gene position: 300546-301460 (Clockwise)

Preceding gene: 195977424

Following gene: 195977426

Centisome position: 14.85

GC content: 44.26

Gene sequence:

>915_bases
ATGATTAAATATCTTTTAAAGCGAGTGGCTATTTTAGTAGTGACACTCTGGGTGGTGATTACCCTGTCCTTCTTTTTGAT
GCAGGTGATGCCTGGAACACCCTATAACAGTCCTAAATTAACTGATGACATGATTGCTATGCTGAATCAGCAGTACGGCT
TAGATAAGCCTCTTTGGCAGCAATATTTGAAGTATTTGTTTGATATTCTTCATGGTGATTTTGGCACCAGCTATCAGTCG
ATTAACCAATCAGTGACAACCCTGATCTCGCAGCGTCTGGGGGTATCTGTACACTTAGGAGTACAGGCGCTTATTGTTGG
TATTAGCTCTGGTTTAGTTGTTGGTGCTGTTTCAGCTCGGAATAAAAATAATAGCATCGATGCCATTTTAAGTGTTATTT
CAACGCTTGGTATTTCCATGCCGTCCTTTATCATTGGCTTGCTGCTGCTGGATTATTTGGGCTTTAAATGGAATTTACTT
CCCTTGTCTGGTTGGGGAAGCTTTGGGCAAACGATTTTGCCGACGCTTGCTTTAGCTATTCCGGTCTTTGCTCAGGTGAC
CCGTTTTTTCCGCAGCGAGATGATCGAGACCTTATCGACAGATTATATTCAGCTAGCTAGGGCGAAGGGCTTGACTAAGC
GTCAGGTGACTCGTCGGCATGCTTACCGCAATTCGATGATTCCTGTGTTAACCTTGGTTGGTCCGATGGCTGCTGGTATT
TTGACGGGATCAGCCCTGATTGAGCAGATTTTTTCCATTCCGGGGATTGGGCAGCAGTTTGTGACCTCTATTCCCACCAA
GGATTACCCGGTGATTATGGGAACAACGATTGTCTATGCGCTAATGCTGATGGTTGCTATTTTGGTAACTGATATGATCA
TTAGTATCGCAGATCCACGTGTTCGTTTGGGATAG

Upstream 100 bases:

>100_bases
GAAGCTTAGGGCTAGCTAGGAGCGTCTATCCTATAAGCCTTGGCAAGCATATTGCAAGAATTGGTCTTAACCAGTTCTTG
CTTTTGGTTAGAGGAAAAAT

Downstream 100 bases:

>100_bases
GAGAGTGCTATGCTAGAAGAAACAAAATCATTTAAGCTTGTTGGAGCAGGGAGCTTAGCGACACAAGAAAAAATTGAAAA
GCCGGCGCTTTCCTTTCTTC

Product: oligopeptide transport system permease protein OppB

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KB [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIKYLLKRVAILVVTLWVVITLSFFLMQVMPGTPYNSPKLTDDMIAMLNQQYGLDKPLWQQYLKYLFDILHGDFGTSYQS
INQSVTTLISQRLGVSVHLGVQALIVGISSGLVVGAVSARNKNNSIDAILSVISTLGISMPSFIIGLLLLDYLGFKWNLL
PLSGWGSFGQTILPTLALAIPVFAQVTRFFRSEMIETLSTDYIQLARAKGLTKRQVTRRHAYRNSMIPVLTLVGPMAAGI
LTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTTIVYALMLMVAILVTDMIISIADPRVRLG

Sequences:

>Translated_304_residues
MIKYLLKRVAILVVTLWVVITLSFFLMQVMPGTPYNSPKLTDDMIAMLNQQYGLDKPLWQQYLKYLFDILHGDFGTSYQS
INQSVTTLISQRLGVSVHLGVQALIVGISSGLVVGAVSARNKNNSIDAILSVISTLGISMPSFIIGLLLLDYLGFKWNLL
PLSGWGSFGQTILPTLALAIPVFAQVTRFFRSEMIETLSTDYIQLARAKGLTKRQVTRRHAYRNSMIPVLTLVGPMAAGI
LTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTTIVYALMLMVAILVTDMIISIADPRVRLG
>Mature_304_residues
MIKYLLKRVAILVVTLWVVITLSFFLMQVMPGTPYNSPKLTDDMIAMLNQQYGLDKPLWQQYLKYLFDILHGDFGTSYQS
INQSVTTLISQRLGVSVHLGVQALIVGISSGLVVGAVSARNKNNSIDAILSVISTLGISMPSFIIGLLLLDYLGFKWNLL
PLSGWGSFGQTILPTLALAIPVFAQVTRFFRSEMIETLSTDYIQLARAKGLTKRQVTRRHAYRNSMIPVLTLVGPMAAGI
LTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTTIVYALMLMVAILVTDMIISIADPRVRLG

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane. Also required for sporulation and competence [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787497, Length=305, Percent_Identity=34.4262295081967, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1789965, Length=337, Percent_Identity=32.3442136498516, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787053, Length=305, Percent_Identity=33.4426229508197, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789888, Length=309, Percent_Identity=30.7443365695793, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1788504, Length=361, Percent_Identity=27.1468144044321, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787761, Length=332, Percent_Identity=29.8192771084337, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787550, Length=278, Percent_Identity=26.2589928057554, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33430; Mature: 33430

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYLLKRVAILVVTLWVVITLSFFLMQVMPGTPYNSPKLTDDMIAMLNQQYGLDKPLWQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
QYLKYLFDILHGDFGTSYQSINQSVTTLISQRLGVSVHLGVQALIVGISSGLVVGAVSAR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEHHCC
NKNNSIDAILSVISTLGISMPSFIIGLLLLDYLGFKWNLLPLSGWGSFGQTILPTLALAI
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
PVFAQVTRFFRSEMIETLSTDYIQLARAKGLTKRQVTRRHAYRNSMIPVLTLVGPMAAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTTIVYALMLMVAILVTDMIISIADPR
HHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VRLG
CCCC
>Mature Secondary Structure
MIKYLLKRVAILVVTLWVVITLSFFLMQVMPGTPYNSPKLTDDMIAMLNQQYGLDKPLWQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
QYLKYLFDILHGDFGTSYQSINQSVTTLISQRLGVSVHLGVQALIVGISSGLVVGAVSAR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEHHCC
NKNNSIDAILSVISTLGISMPSFIIGLLLLDYLGFKWNLLPLSGWGSFGQTILPTLALAI
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
PVFAQVTRFFRSEMIETLSTDYIQLARAKGLTKRQVTRRHAYRNSMIPVLTLVGPMAAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTTIVYALMLMVAILVTDMIISIADPR
HHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VRLG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]