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The map label for this gene is oppA [H]

Identifier: 195977424

GI number: 195977424

Start: 298721

End: 300379

Strand: Direct

Name: oppA [H]

Synonym: Sez_0279

Alternate gene names: 195977424

Gene position: 298721-300379 (Clockwise)

Preceding gene: 195977423

Following gene: 195977425

Centisome position: 14.76

GC content: 42.98

Gene sequence:

>1659_bases
ATGACAGTACTAGGAACAAAAGCATGTAAGCGTCTGGGCTTAGCGGCAGTTACGCTAGCCTCTGTTGCTGCCTTGATGGC
TTGTGGAAATAAGCAATCAGCGTCAACAGACAAAAAGAGTGAGATTAATTGGTATACGCCAACTGAGATCATCACACTTG
ATATTTCAAAAAATACAGATACCTATTCTGCTTTGGCTATTGGTAATTCTGGCAGTAACCTTTTGCGTGCTGATGCTAAA
GGGAAATTACAGCCTGATTTAGCTGAAAAGGTTGATGTGTCAGAGGACGGCTTGACCTATACAGCAACCCTGCGAGATGG
CTTGAAATGGTCTGATGGTAGTGATCTAACAGCAGAGGACTTTGTGTACAGTTGGCAGCGAATGGTCGATCCTAAGACAG
CCTCAGAGTACGCTTACTTGGCAACTGAGTCACATCTGAAAAACGCAGAGGACATTAATAGCGGGAAAAATCCTGATCTA
GACTCTCTAGGGGTAAAGGCTGATGGGAATAAGGTTATTTTTACCTTAACGGAGCCGGCACCACAATTTAAGAGCTTGCT
ATCCTTCTCTAACTTTGTCCCTCAAAAAGAATCCTTTGTCAAGGACGCTGGCAAGGACTATGGGACAACATCAGAAAAAC
AAATTTATTCTGGTCCTTATATTGTCAAGGACTGGAATGGCACTAGCGGAACCTTTAAGCTAGTAAAGAATAAAAACTAT
TGGGACGCCAAAAACGTCAAAACTGAGACAGTTAATGTTCAAACGGTTAAAAAGCCAGATACAGCTGTTCAAATGTACAA
GCAAGGTAAGCTAGACTTTGCAAATATTTCTGGTACCTCAGCTATTTACAATGCTAATAAAAAGCATAAGGACGTTGTTC
CGGTTCTTGAGGCAACAACAGCCTATATCGTATATAACCAGACAGGAGCTATTGAAGGCTTGAACAGTCTTAAAATTCGT
CAAGCCTTGAATTTGGCAACAGACCGTAAGGGAATTGTATCTGCGGCTGTTGATACAGGCTCAAAGCCGGCTACAGCGCT
GGTTCCTACAGGTCTTGCTAAATTATCTGACGGAACAGATCTAACAGAGCATGTGGCACCTGGCTATAAATACGATGACA
AGGAGGCAGCAAAGCTCTTCAAGGAAGGCTTAGCAGAGCTGGGCAAGGATGCCTTGACAATCACCATCACAGCTGATGCT
GATGCGCCTGCTGCCAAGTCTGCAGTGGATTACATCAAGGAAACCTGGGAAACAGCTCTTCCTGGCTTGACCGTTGAAGA
GAAATTTGTTCCTTTCAAGCAACGTCTTGAGGATACTAAAAACCAAAACTTTGAGGTTGCAGTTGTTCTTTGGGGTGGTG
ATTATCCAGAAGGGTCTACCTTCTATGGCTTGTTTAAATCAGGTTCTGCTTATAACTATGGCAAATTTACGAATGCAGAC
TATGATGCGGCCTACAATAAGGCTCTAACAACAGATGCCTTGAATACAGATGCGGCTGCTGATGATTATAAGGCGGCAGA
AAAAGCCCTTTATGACAATGCTCTTTACAATCCCCTTTACTTCCGTAGTGGTGAGGGCTTGCAAAATCCAAGCATCAAAG
GTCTTATCCGTAATTCAACTGGTCTAAATGTTGACTTTACTTACGCTTATAAGGAATAA

Upstream 100 bases:

>100_bases
AAATCAAAAATTTTCAGATAAATCTATTTCCTTTTTTCAGAATTATGGTATAATCTAATTTAATTATTTTAAATAACTAT
TTTGTTAGGAGGAAGGTTAT

Downstream 100 bases:

>100_bases
CAATAATATTATCGTAAGCTTATACTGACCTAGCAAGGACAGCTCCTTGGTGGGAAGTGTTTAAGAGAAGCTTAGGGCTA
GCTAGGAGCGTCTATCCTAT

Product: oligopeptide-binding protein OppA precursor

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTDTYSALAIGNSGSNLLRADAK
GKLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAEDFVYSWQRMVDPKTASEYAYLATESHLKNAEDINSGKNPDL
DSLGVKADGNKVIFTLTEPAPQFKSLLSFSNFVPQKESFVKDAGKDYGTTSEKQIYSGPYIVKDWNGTSGTFKLVKNKNY
WDAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTSAIYNANKKHKDVVPVLEATTAYIVYNQTGAIEGLNSLKIR
QALNLATDRKGIVSAAVDTGSKPATALVPTGLAKLSDGTDLTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADA
DAPAAKSAVDYIKETWETALPGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPEGSTFYGLFKSGSAYNYGKFTNAD
YDAAYNKALTTDALNTDAAADDYKAAEKALYDNALYNPLYFRSGEGLQNPSIKGLIRNSTGLNVDFTYAYKE

Sequences:

>Translated_552_residues
MTVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTDTYSALAIGNSGSNLLRADAK
GKLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAEDFVYSWQRMVDPKTASEYAYLATESHLKNAEDINSGKNPDL
DSLGVKADGNKVIFTLTEPAPQFKSLLSFSNFVPQKESFVKDAGKDYGTTSEKQIYSGPYIVKDWNGTSGTFKLVKNKNY
WDAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTSAIYNANKKHKDVVPVLEATTAYIVYNQTGAIEGLNSLKIR
QALNLATDRKGIVSAAVDTGSKPATALVPTGLAKLSDGTDLTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADA
DAPAAKSAVDYIKETWETALPGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPEGSTFYGLFKSGSAYNYGKFTNAD
YDAAYNKALTTDALNTDAAADDYKAAEKALYDNALYNPLYFRSGEGLQNPSIKGLIRNSTGLNVDFTYAYKE
>Mature_551_residues
TVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTDTYSALAIGNSGSNLLRADAKG
KLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAEDFVYSWQRMVDPKTASEYAYLATESHLKNAEDINSGKNPDLD
SLGVKADGNKVIFTLTEPAPQFKSLLSFSNFVPQKESFVKDAGKDYGTTSEKQIYSGPYIVKDWNGTSGTFKLVKNKNYW
DAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTSAIYNANKKHKDVVPVLEATTAYIVYNQTGAIEGLNSLKIRQ
ALNLATDRKGIVSAAVDTGSKPATALVPTGLAKLSDGTDLTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADAD
APAAKSAVDYIKETWETALPGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPEGSTFYGLFKSGSAYNYGKFTNADY
DAAYNKALTTDALNTDAAADDYKAAEKALYDNALYNPLYFRSGEGLQNPSIKGLIRNSTGLNVDFTYAYKE

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=529, Percent_Identity=28.7334593572779, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=29.1588785046729, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1787495, Length=477, Percent_Identity=31.0272536687631, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787052, Length=380, Percent_Identity=27.8947368421053, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789966, Length=451, Percent_Identity=25.4988913525499, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59935; Mature: 59804

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCC
TYSALAIGNSGSNLLRADAKGKLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAED
CEEEEEECCCCCCEEEECCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
FVYSWQRMVDPKTASEYAYLATESHLKNAEDINSGKNPDLDSLGVKADGNKVIFTLTEPA
HHHHHHHHCCCCCCCCEEEEEEHHHCCCCHHCCCCCCCCHHHCCEEECCCEEEEEECCCC
PQFKSLLSFSNFVPQKESFVKDAGKDYGTTSEKQIYSGPYIVKDWNGTSGTFKLVKNKNY
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEECCCEEEECCCCCCCEEEEEECCCC
WDAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTSAIYNANKKHKDVVPVLEATT
CCCCCCEEEEEEEEEECCCHHHHHHHHCCCCEEECCCCCHHEECCCCCCCCCHHHEECCE
AYIVYNQTGAIEGLNSLKIRQALNLATDRKGIVSAAVDTGSKPATALVPTGLAKLSDGTD
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCHHHCCCCCC
LTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADADAPAAKSAVDYIKETWETAL
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHC
PGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPEGSTFYGLFKSGSAYNYGKFTNAD
CCCEEHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
YDAAYNKALTTDALNTDAAADDYKAAEKALYDNALYNPLYFRSGEGLQNPSIKGLIRNST
CHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCC
GLNVDFTYAYKE
CCEEEEEEEECC
>Mature Secondary Structure 
TVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTD
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCC
TYSALAIGNSGSNLLRADAKGKLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAED
CEEEEEECCCCCCEEEECCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
FVYSWQRMVDPKTASEYAYLATESHLKNAEDINSGKNPDLDSLGVKADGNKVIFTLTEPA
HHHHHHHHCCCCCCCCEEEEEEHHHCCCCHHCCCCCCCCHHHCCEEECCCEEEEEECCCC
PQFKSLLSFSNFVPQKESFVKDAGKDYGTTSEKQIYSGPYIVKDWNGTSGTFKLVKNKNY
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEECCCEEEECCCCCCCEEEEEECCCC
WDAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTSAIYNANKKHKDVVPVLEATT
CCCCCCEEEEEEEEEECCCHHHHHHHHCCCCEEECCCCCHHEECCCCCCCCCHHHEECCE
AYIVYNQTGAIEGLNSLKIRQALNLATDRKGIVSAAVDTGSKPATALVPTGLAKLSDGTD
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCHHHCCCCCC
LTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADADAPAAKSAVDYIKETWETAL
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHC
PGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPEGSTFYGLFKSGSAYNYGKFTNAD
CCCEEHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
YDAAYNKALTTDALNTDAAADDYKAAEKALYDNALYNPLYFRSGEGLQNPSIKGLIRNST
CHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCC
GLNVDFTYAYKE
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]