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The map label for this gene is glnA [H]

Identifier: 195977423

GI number: 195977423

Start: 297142

End: 298488

Strand: Direct

Name: glnA [H]

Synonym: Sez_0278

Alternate gene names: 195977423

Gene position: 297142-298488 (Clockwise)

Preceding gene: 195977422

Following gene: 195977424

Centisome position: 14.68

GC content: 42.98

Gene sequence:

>1347_bases
ATGGCAATCACAGCAGCTGATGTTCGTCGCGAGGTCAAAGAAAAGCATGTGACCTTTCTTCGGTTGATGTTTACGGACAT
TATGGGTGTTATGAAAAATGTTGAGATACCTGCAACTGAAGAGCAATTAGATAAAGTCTTATCTAATAAGGTCATGTTTG
ATGGCTCCTCGATTGAAGGCTTTGTGCGTATCAATGAATCAGATATGTATCTCTATCCTGATTTAGACACTTGGATCGTC
TTTCCTTGGGGAGATGAAAATGGAGCTGTTGGTGGCTTGATTTGTGATATTTACACTGCTGAAGGCAAGCCCTTTGCTGG
TGACCCACGTGGCAACCTAAAAAGAGCCTTGAAGCATATGGATGAGGTTGGCTATCAGTCCTTTAACCTTGGTCCAGAGC
CAGAATTTTTCTTGTTTAAGATAGATGAAGCTGGAGAGCCAACGCTTGAGGTCAATGACAAGGGAGGCTATTTTGACCTC
GCTCCGATTGATCTGGCAGACAATACCCGTCGTGAAATTGTTAATGTCTTGACCAAAATGGGCTTTGAGGTCGAAGCCAG
CCACCATGAGGTAGCTGTCGGTCAGCATGAAATTGACTTTAAATATGCAGATGCCTTGAAGGCCTGTGATAATATTCAGA
TCTTTAAGCTTGTCGTCAAGACAATTGCTCGTGAGCACGGGCTCTACGCCACCTTTATGGCAAAGCCGAAATTTGGTATT
GCCGGCTCAGGTATGCACTGCAACATGTCCTTATTTGATAAAGAGGGCAACAATGCCTTTTATGATGAAAATGATAAGCG
TGGCATGAAGCTATCAGATGATGCCTATTATTTCTTAGGTGGGTTGATGAAGCATGCTTACAATTACACCGCTATTACAA
ATCCAACAGTTAATTCTTACAAGCGCTTGGTTCCTGGTTATGAGGCTCCTGTTTATGTTGCTTGGGCAGGAAGCAATCGT
TCACCTCTTATTCGTGTTCCTGCTTCTCGTGGTATGGGGACTCGTTTAGAATTGCGTTCGGTTGATCCAACAGCCAATCC
TTATTTAGCACTTGCAGTTTTACTAGAGGCAGGCTTAGATGGTATCATTAATAAAATTGAAGCGCCGGCACCGATTGAGG
CTAATATTTACATGATGACAGCAGAGGAGCGCAATGCTGCTGGCATTGTTGATTTACCGTCAACACTCCATAATGCTCTA
AAGGCGCTTCAAAAGGATGAGGTCATTCAAAAGGCTTTAGGTGATCATATTTACACCAACTTTTTAGAGGCAAAACGGAT
TGAATGGTCCTCCTATGCGACCTTTGTTTCGCAATGGGAGATTGATAATTACTTACACAACTATTAG

Upstream 100 bases:

>100_bases
TGAGCTTCTTAATCAAGGTGGCTTTTCCACCCCTTCACAGCATATTGGGAATTTTCGTATCTAGCATACGACTGGCATGA
AAGAAAAAGGAGATTGATTA

Downstream 100 bases:

>100_bases
AAAAACAAAACTCACTCAGCTTTTTGAGTGAGTTTTTTATGCTTTTATTCAGTAGATATGGTTATTTATGCTTATCGTTC
CTTTTATACGCTATGATGAC

Product: glutamine synthetase GlnA

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAITAADVRREVKEKHVTFLRLMFTDIMGVMKNVEIPATEEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDENGAVGGLICDIYTAEGKPFAGDPRGNLKRALKHMDEVGYQSFNLGPEPEFFLFKIDEAGEPTLEVNDKGGYFDL
APIDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYADALKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
AGSGMHCNMSLFDKEGNNAFYDENDKRGMKLSDDAYYFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNR
SPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPAPIEANIYMMTAEERNAAGIVDLPSTLHNAL
KALQKDEVIQKALGDHIYTNFLEAKRIEWSSYATFVSQWEIDNYLHNY

Sequences:

>Translated_448_residues
MAITAADVRREVKEKHVTFLRLMFTDIMGVMKNVEIPATEEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDENGAVGGLICDIYTAEGKPFAGDPRGNLKRALKHMDEVGYQSFNLGPEPEFFLFKIDEAGEPTLEVNDKGGYFDL
APIDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYADALKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
AGSGMHCNMSLFDKEGNNAFYDENDKRGMKLSDDAYYFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNR
SPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPAPIEANIYMMTAEERNAAGIVDLPSTLHNAL
KALQKDEVIQKALGDHIYTNFLEAKRIEWSSYATFVSQWEIDNYLHNY
>Mature_447_residues
AITAADVRREVKEKHVTFLRLMFTDIMGVMKNVEIPATEEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVF
PWGDENGAVGGLICDIYTAEGKPFAGDPRGNLKRALKHMDEVGYQSFNLGPEPEFFLFKIDEAGEPTLEVNDKGGYFDLA
PIDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYADALKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGIA
GSGMHCNMSLFDKEGNNAFYDENDKRGMKLSDDAYYFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRS
PLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPAPIEANIYMMTAEERNAAGIVDLPSTLHNALK
ALQKDEVIQKALGDHIYTNFLEAKRIEWSSYATFVSQWEIDNYLHNY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=444, Percent_Identity=26.8018018018018, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790301, Length=444, Percent_Identity=42.3423423423423, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=24.1228070175439, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50177; Mature: 50045

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00180 GLNA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITAADVRREVKEKHVTFLRLMFTDIMGVMKNVEIPATEEQLDKVLSNKVMFDGSSIEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRINESDMYLYPDLDTWIVFPWGDENGAVGGLICDIYTAEGKPFAGDPRGNLKRALKHM
EEEECCCCEEEECCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHH
DEVGYQSFNLGPEPEFFLFKIDEAGEPTLEVNDKGGYFDLAPIDLADNTRREIVNVLTKM
HHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHC
GFEVEASHHEVAVGQHEIDFKYADALKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
CCEEECCCCEEEECCEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AGSGMHCNMSLFDKEGNNAFYDENDKRGMKLSDDAYYFLGGLMKHAYNYTAITNPTVNSY
CCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCEEECCCCHHHH
KRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLD
HHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHH
GIINKIEAPAPIEANIYMMTAEERNAAGIVDLPSTLHNALKALQKDEVIQKALGDHIYTN
HHHHHCCCCCCCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLEAKRIEWSSYATFVSQWEIDNYLHNY
HHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AITAADVRREVKEKHVTFLRLMFTDIMGVMKNVEIPATEEQLDKVLSNKVMFDGSSIEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRINESDMYLYPDLDTWIVFPWGDENGAVGGLICDIYTAEGKPFAGDPRGNLKRALKHM
EEEECCCCEEEECCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHH
DEVGYQSFNLGPEPEFFLFKIDEAGEPTLEVNDKGGYFDLAPIDLADNTRREIVNVLTKM
HHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHC
GFEVEASHHEVAVGQHEIDFKYADALKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
CCEEECCCCEEEECCEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AGSGMHCNMSLFDKEGNNAFYDENDKRGMKLSDDAYYFLGGLMKHAYNYTAITNPTVNSY
CCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCEEECCCCHHHH
KRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLD
HHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHH
GIINKIEAPAPIEANIYMMTAEERNAAGIVDLPSTLHNALKALQKDEVIQKALGDHIYTN
HHHHHCCCCCCCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLEAKRIEWSSYATFVSQWEIDNYLHNY
HHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]