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The map label for this gene is nanA [H]

Identifier: 195977398

GI number: 195977398

Start: 272454

End: 273374

Strand: Direct

Name: nanA [H]

Synonym: Sez_0251

Alternate gene names: 195977398

Gene position: 272454-273374 (Clockwise)

Preceding gene: 195977397

Following gene: 195977399

Centisome position: 13.46

GC content: 44.84

Gene sequence:

>921_bases
ATGAAAATTTTAACCAAATATCAAGGGATTATCCCTGCCTTTTACGCCTGCTATGACGCTGAGGGCAATATTAGTCCAGA
GCGTGTTCGTGCTTTGACACAATATTATATTGATAAGGGTGTCCAAGGGCTTTATGTCAATGGCTCATCAGGCGAGTGTA
TCTACCAAAGTGTGGCTGATCGTAAGCTGGTTTTGGAGCATGTGATGGCAGTAGCCAAGGGTAAGCTAACAATCATCAAT
CATGTTGCCTGCAACAATACCAAAGACAGCATTGAGCTGGCAAAGCATGCTGAGGCCTTAGGTGTTGATGCTATTGCAGC
GATTCCACCGATTTATTTCCGTTTGCCTGAGCATGCTGTGGCTGCTTATTGGAATGCGATTTCAGCTGCTGCTCCAAATA
CTGATTTTGTGATTTACAATATTCCGCAATTGGCTGGTATTGCCTTGACCCCAAGCCTCTATCAGGAAATGCTTAAAAAC
GAGCGGGTCATAGGGGTCAAAAATTCCTCCATGCCTGTTCAGGATATTCAAACATTTTGTGCTATCGGAGGTGATGATCA
CCTTGTCTTTAATGGACCGGACGAGCAATTCTTAGGGGGACGCCTCATGGGAGCCTCTGCTGGTATTGGTGGTACCTATG
GAGCGATGCCAGAGCTTTTCTTACGCTTAAATCAATTGATCAAGGACAAGGACCTAGAAACTGCTAAGGCCCTACAGTAT
GCTATCAATGATATTATCGGGCTATTGGTTTCTGCTCATGGCAATATGTATGGTGTTATCAAGGAGGTGCTGCGCATTAA
TGAGGGTCTGGATATTGGTTCTGTTCGGTCACCTTTACCAGAGCTAACAGAGGCTGATTTGGTGATCTGTCAGCAGGCTG
CAGCCTTGATTAAGCAGACCAAGGAACAATTTTGCTATTAA

Upstream 100 bases:

>100_bases
GGCATTAGCAGCCTAACCTATCCTTATGTCGTCATTATGGAAAACCTTCTTCAAAAGGTACAACTTCATTAAAAATAAGA
TTTAGAAAAAGAGTGTCATT

Downstream 100 bases:

>100_bases
AATGAGGTGATCAAATGAAGCATTATCTTGCTATTGACATTGGCGGTACAGCCATCAAGTATGGCCTTATGACAGAGACA
GGAGATTTGCTTGAAAAGCA

Product: N-acetylneuraminate lyase NanA

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKILTKYQGIIPAFYACYDAEGNISPERVRALTQYYIDKGVQGLYVNGSSGECIYQSVADRKLVLEHVMAVAKGKLTIIN
HVACNNTKDSIELAKHAEALGVDAIAAIPPIYFRLPEHAVAAYWNAISAAAPNTDFVIYNIPQLAGIALTPSLYQEMLKN
ERVIGVKNSSMPVQDIQTFCAIGGDDHLVFNGPDEQFLGGRLMGASAGIGGTYGAMPELFLRLNQLIKDKDLETAKALQY
AINDIIGLLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLPELTEADLVICQQAAALIKQTKEQFCY

Sequences:

>Translated_306_residues
MKILTKYQGIIPAFYACYDAEGNISPERVRALTQYYIDKGVQGLYVNGSSGECIYQSVADRKLVLEHVMAVAKGKLTIIN
HVACNNTKDSIELAKHAEALGVDAIAAIPPIYFRLPEHAVAAYWNAISAAAPNTDFVIYNIPQLAGIALTPSLYQEMLKN
ERVIGVKNSSMPVQDIQTFCAIGGDDHLVFNGPDEQFLGGRLMGASAGIGGTYGAMPELFLRLNQLIKDKDLETAKALQY
AINDIIGLLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLPELTEADLVICQQAAALIKQTKEQFCY
>Mature_306_residues
MKILTKYQGIIPAFYACYDAEGNISPERVRALTQYYIDKGVQGLYVNGSSGECIYQSVADRKLVLEHVMAVAKGKLTIIN
HVACNNTKDSIELAKHAEALGVDAIAAIPPIYFRLPEHAVAAYWNAISAAAPNTDFVIYNIPQLAGIALTPSLYQEMLKN
ERVIGVKNSSMPVQDIQTFCAIGGDDHLVFNGPDEQFLGGRLMGASAGIGGTYGAMPELFLRLNQLIKDKDLETAKALQY
AINDIIGLLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLPELTEADLVICQQAAALIKQTKEQFCY

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=285, Percent_Identity=28.7719298245614, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1789620, Length=288, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI87082415, Length=239, Percent_Identity=28.4518828451883, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786463, Length=238, Percent_Identity=26.0504201680672, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788823, Length=194, Percent_Identity=22.680412371134, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33281; Mature: 33281

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILTKYQGIIPAFYACYDAEGNISPERVRALTQYYIDKGVQGLYVNGSSGECIYQSVAD
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKLVLEHVMAVAKGKLTIINHVACNNTKDSIELAKHAEALGVDAIAAIPPIYFRLPEHAV
HHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHCCCCEEECCHHHH
AAYWNAISAAAPNTDFVIYNIPQLAGIALTPSLYQEMLKNERVIGVKNSSMPVQDIQTFC
HHHHHHHHHCCCCCCEEEEECHHHCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AIGGDDHLVFNGPDEQFLGGRLMGASAGIGGTYGAMPELFLRLNQLIKDKDLETAKALQY
HCCCCCEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AINDIIGLLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLPELTEADLVICQQAAALIKQT
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
KEQFCY
HHHHCC
>Mature Secondary Structure
MKILTKYQGIIPAFYACYDAEGNISPERVRALTQYYIDKGVQGLYVNGSSGECIYQSVAD
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKLVLEHVMAVAKGKLTIINHVACNNTKDSIELAKHAEALGVDAIAAIPPIYFRLPEHAV
HHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHCCCCEEECCHHHH
AAYWNAISAAAPNTDFVIYNIPQLAGIALTPSLYQEMLKNERVIGVKNSSMPVQDIQTFC
HHHHHHHHHCCCCCCEEEEECHHHCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AIGGDDHLVFNGPDEQFLGGRLMGASAGIGGTYGAMPELFLRLNQLIKDKDLETAKALQY
HCCCCCEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AINDIIGLLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLPELTEADLVICQQAAALIKQT
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
KEQFCY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA