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The map label for this gene is sgaC [H]

Identifier: 195977317

GI number: 195977317

Start: 184538

End: 185023

Strand: Direct

Name: sgaC [H]

Synonym: Sez_0170

Alternate gene names: 195977317

Gene position: 184538-185023 (Clockwise)

Preceding gene: 195977316

Following gene: 195977318

Centisome position: 9.12

GC content: 46.91

Gene sequence:

>486_bases
ATGAATTTAAAAAAAGCATTTATCGAAAACAACTCTATTCGCTTGGGTTTGTCAGCAGCTAGCTGGCAGGAAGCGGTTAA
GCTGGCCGTTCAGCCTTTGATTGACAGCGGGGCGGTGACCTCTGAATATTATGACGCTATTATCGCGTCAACTGAAAAAT
ACGGGCCCTATTATGTTCTTATGCCGGGAATGGCCATGCCACACGCAGAGGCTGGTGTAGGGGTCAAGCGAAATGCCTTT
GCGCTTATCACGCTGAGTCAGCCAGTGACATTTTCAGATGGTAAAGAGGTTTCGGTATTGCTAACCTTGGCAGCAACTGA
TCCTTCTATTCATACGACGGTTGCGATTCCACAGATTGTCGCCCTCTTTGAATTGGAAGACGCTATTTCGCGCTTAATTG
CCTGTCGCACTCCAGAGGAGGTTTTGGCTCTGGTGGATGAGTCCAAAAACAGTCCTTATTTAGAAGGCTTGGATTTGGAC
TCTTAA

Upstream 100 bases:

>100_bases
AGTTGTGAAGAGAGGTTCCAGCTCTTTCTCTTTATCAGCTAGATAGGAGTGGGGCAAAAAGTGTCTAGCCAAAGAATTTG
ATTTAGAAAGGATTAGTTAT

Downstream 100 bases:

>100_bases
ACAAGCCATCATCACTAGATAAATCAAACATAACAGAAAGGGTAGGAGCTCACTACTAAGCATTGAAGGCGGTGCTACGG
GCTCTTGGTAAAAAATCATG

Product: ascorbate-specific phosphotransferase enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNLKKAFIENNSIRLGLSAASWQEAVKLAVQPLIDSGAVTSEYYDAIIASTEKYGPYYVLMPGMAMPHAEAGVGVKRNAF
ALITLSQPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIVALFELEDAISRLIACRTPEEVLALVDESKNSPYLEGLDLD
S

Sequences:

>Translated_161_residues
MNLKKAFIENNSIRLGLSAASWQEAVKLAVQPLIDSGAVTSEYYDAIIASTEKYGPYYVLMPGMAMPHAEAGVGVKRNAF
ALITLSQPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIVALFELEDAISRLIACRTPEEVLALVDESKNSPYLEGLDLD
S
>Mature_161_residues
MNLKKAFIENNSIRLGLSAASWQEAVKLAVQPLIDSGAVTSEYYDAIIASTEKYGPYYVLMPGMAMPHAEAGVGVKRNAF
ALITLSQPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIVALFELEDAISRLIACRTPEEVLALVDESKNSPYLEGLDLD
S

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=151, Percent_Identity=46.3576158940397, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1790755, Length=132, Percent_Identity=35.6060606060606, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1789302, Length=112, Percent_Identity=38.3928571428571, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17314; Mature: 17314

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKKAFIENNSIRLGLSAASWQEAVKLAVQPLIDSGAVTSEYYDAIIASTEKYGPYYVL
CCCCHHEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEE
MPGMAMPHAEAGVGVKRNAFALITLSQPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIV
ECCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCEEEEEEEEEECCCCCEEEEHHHHHH
ALFELEDAISRLIACRTPEEVLALVDESKNSPYLEGLDLDS
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLKKAFIENNSIRLGLSAASWQEAVKLAVQPLIDSGAVTSEYYDAIIASTEKYGPYYVL
CCCCHHEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEE
MPGMAMPHAEAGVGVKRNAFALITLSQPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIV
ECCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCEEEEEEEEEECCCCCEEEEHHHHHH
ALFELEDAISRLIACRTPEEVLALVDESKNSPYLEGLDLDS
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]