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The map label for this gene is sgaB [H]

Identifier: 195977316

GI number: 195977316

Start: 184043

End: 184321

Strand: Direct

Name: sgaB [H]

Synonym: Sez_0169

Alternate gene names: 195977316

Gene position: 184043-184321 (Clockwise)

Preceding gene: 195977315

Following gene: 195977317

Centisome position: 9.09

GC content: 43.37

Gene sequence:

>279_bases
ATGGTTAAGGTTCTTACAGCATGCGGTAATGGCATGGGATCATCAATGGTTATTAAAATGAAGGTTGAAAACGCGCTTCG
TCAGCTTGGGGTAACTGATATTCAGTCTGCTTCTTGCTCAGTGGGTGAGGCAAAGGGACTTGCTTCAGGCTATGACATTG
TGGTTGCTTCTAATCACCTGATTCATGAATTAGATGGGCGTACAAAGGGTCATTTGGTTGGACTAGATAATCTTATGGAC
GACAATGAAATCAAAACCAAGCTACAGGCGGTGCTTTAG

Upstream 100 bases:

>100_bases
CTGCTTATTCGTTTTTTAGCACAGGCTGGTAGGAGTTTGTCGATATACTTCTGCAGGTCTTCATTCATATCAAATTATCA
TATATTCGGAGGAAAATAAA

Downstream 100 bases:

>100_bases
AGCGGCCCTGATGACATCAGTGCTGCTCATGGCAAATGATAGTGAGCCTAAGAGCAGCCACTTGGCTGTAAGCTAAGCTG
TAGCAATCAACTTTCAGTAA

Product: PTS system, 3-keto-L-gulonate/L-ascorbate specific IIB component (SgaB)

Products: NA

Alternate protein names: Ascorbate-specific PTS system EIIB component [H]

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEAKGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMD
DNEIKTKLQAVL

Sequences:

>Translated_92_residues
MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEAKGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMD
DNEIKTKLQAVL
>Mature_92_residues
MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEAKGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMD
DNEIKTKLQAVL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3414

COG function: function code G; Phosphotransferase system, galactitol-specific IIB component

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790638, Length=91, Percent_Identity=40.6593406593407, Blast_Score=69, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 9710; Mature: 9710

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEAKGLASGYDIVVASNHL
CEEEEECCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCE
IHELDGRTKGHLVGLDNLMDDNEIKTKLQAVL
EEECCCCCCCEEEECCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEAKGLASGYDIVVASNHL
CEEEEECCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCE
IHELDGRTKGHLVGLDNLMDDNEIKTKLQAVL
EEECCCCCCCEEEECCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633; 10954595; 11271496 [H]