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The map label for this gene is leuS [H]

Identifier: 195977313

GI number: 195977313

Start: 179254

End: 181755

Strand: Direct

Name: leuS [H]

Synonym: Sez_0166

Alternate gene names: 195977313

Gene position: 179254-181755 (Clockwise)

Preceding gene: 195977311

Following gene: 195977314

Centisome position: 8.86

GC content: 47.72

Gene sequence:

>2502_bases
ATGACATTTTACAATCACAAAGCCATTGAGCCTAAGTGGCAGGCCTTTTGGGCTGATAATCATACCTTTAAAACGGGGAC
AGATGCCTCAAAGCCTAAATTTTATGCTTTAGATATGTTTCCTTATCCTTCCGGTGCAGGTCTTCATGTTGGGCATCCTG
AGGGTTACACTGCAACTGATATTTTAAGCCGCTTCAAGCGTGCACAGGGTTATAATGTCCTCCACCCAATGGGCTGGGAT
GCCTTTGGTCTGCCTGCTGAGCAATACGCGATGGATACTGGTAATGACCCTGCTGAGTTTACAGCAGAAAACATTGCCAA
CTTTAAACGTCAGATCAATGCTCTTGGCTTCTCGTATGACTGGGATCGTGAGATCAATACAACTGATCCTAGTTACTACA
AATGGACACAGTGGATTTTCACAAAATTGTATGAAAAAGGCCTAGCCTATGAAGCTGAGGTGCCTGTTAACTGGGTTGAA
GAGCTGGGAACAGCTATTGCGAATGAGGAGGTTCTACCTGATGGCACATCAGAGCGTGGAGGCTATCCAGTTGTCCGTAA
GCCCATGCGCCAATGGATGCTCAAAATCACAGCCTACGCAGAGCGCTTGCTAGCAGATCTGGAGGAAGTGGATTGGCCAG
AGTCGATCAAGGACATGCAGCGCAACTGGATTGGCAAATCAACAGGGGCTAATGTAACCTTCAAGGTCAAAGATACTGAT
AAAGACTTCACTGTCTTTACCACACGTCCGGATACCTTGTTTGGTGCAACCTATGCTGTTTTAGCACCAGAACATGCCTT
GGTAGATAGCATTACCACAGCAGAGCAAGCAGCAGCAGTTGCCGAATACAAGCGCCAAGCCAGCCTGAAGTCTGATCTTG
CTCGTACCGACCTTGCCAAGGAAAAGACAGGTGTCTGGACAGGAGCCTATGCGATCAACCCGGTCAATGGCAAAGAAATG
CCTATCTGGATTGCGGATTACGTGCTTGCAAGCTACGGTACCGGCGCTATCATGGCAGTGCCAGCGCATGATGAGCGTGA
TTGGGCCTTTGCAAAGCAATTCAATCTTGAGATTATTCCAGTTCTTGAAGGGGGCAATGTTGATGAGGCTGCCTACACAG
AGGACGGTATTCACATTAATTCTGGCTTCCTAGACGGTCTTGATAAGGCTTGTGCCATTACTAAAATGGTGGCGTGGCTG
GAGGAGACTGGTGTTGGTCACGAAAAGGTCTCTTATCGCCTACGTGACTGGCTGTTCAGTCGTCAGCGCTACTGGGGTGA
GCCAATCCCAATCATTCATTGGGAGGACGGTACCTCTACAGCAGTTCCTGAGCAGGACTTACCACTTGTTTTGCCAGTGA
CTAAGGATATTCGCCCATCTGGTACAGGAGAATCACCACTAGCTAACTTGACTGACTGGCTTGAGGTGACCCGCGAGGAT
GGGGTCAAAGGGCGTCGTGAGACCAACACCATGCCACAGTGGGCTGGCTCAAGCTGGTATTACCTTCGCTATATTGACCC
ACACAATGATGAGCAATTAGCTGATAAGGACCTGTTAAAGCAATGGCTGCCAGTAGATATTTACATTGGTGGTGCTGAGC
ACGCAGTGCTTCATCTGCTTTATGCACGCTTCTGGCACAAGGTACTCTATGATCTGGGTGTAGTGCCAACCAAGGAGCCC
TTCCAAAAGCTCTTTAATCAAGGCATGATTTTGGGAACCAGCTATCGCGACCATCGTGGGGCCTTGGTCGCAACGGATAA
GGTTGACAAGCGTGATGGTAGCTTCTTCCACATGGAAACAGGCGAGGAGCTTGAGCAGGCCCCTGCTAAGATGTCTAAGT
CTCTGAAAAACGTTGTCAACCCAGATGACGTAGTGGAGCAGTACGGAGCAGATACCCTGCGTGTGTATGAAATGTTTATG
GGGCCACTTGATGCGTCCATTGCTTGGTCTGAAGAAGGCCTTGAGGGAGCACGCAAGTTCCTAGATCGTGTTTACCGTTT
GATCACGACAAAGGAAATTGTTGCAGAAAATAGCGGAGCTTTAGATAAGGCTTACCATGAGACTGTTAAGGCGGTCACAG
AACAAATCGAAGGCATGAAATTCAACACTGCTATTGCCCAGCTCATGATTTTTGTCAATGCAGCCAATAAAGAGGACGAG
CTTTATGTGGCATACGCCAAAGGCTTCGTTCAATTGCTAGCCCCATTTGCCCCACACTTGGGTGAAGAATTATGGCAGAT
CCTGACAGCTTCAGGTCAATCCATCTCGTATGTTGCATGGCCAACCCATGACGATAGTAAGCTCGTTGAAAATGATGTTG
AAATCGTTGTTCAAATTAAAGGAAAGGTCAAGGCTAAGCTTGTCGTGGCAAAAGACCTCTCAAGAGAAGAGCTAGAAAAA
GTAGCCCTAGCACATGATAAAATACAGGCTGAAATTGCAGGCAAGGAAGTGGCAAAGGTCATCGTTGTTCCTAATAAGCT
CGTTAATATTGTGGTGAAATAA

Upstream 100 bases:

>100_bases
GTGATGCCGACGTCGTGGCAGCAATATTGCTTGCCCATTCCTAAAAGTCTGATATCGTGCTATAATAAGAAGACTAGACT
TTTTACATGGGAGAATTCAC

Downstream 100 bases:

>100_bases
AGACCTTAGCAAAAAATGCCTTCCTTATAATTTTGAGCCCAATACTATAAAAAGCTGATAACCACTCGTTTACATGAGTG
GTTATTCTTGTTTTGTAACA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MTFYNHKAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGYNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREINTTDPSYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLADLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
KDFTVFTTRPDTLFGATYAVLAPEHALVDSITTAEQAAAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEM
PIWIADYVLASYGTGAIMAVPAHDERDWAFAKQFNLEIIPVLEGGNVDEAAYTEDGIHINSGFLDGLDKACAITKMVAWL
EETGVGHEKVSYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPEQDLPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNDEQLADKDLLKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDLGVVPTKEP
FQKLFNQGMILGTSYRDHRGALVATDKVDKRDGSFFHMETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGARKFLDRVYRLITTKEIVAENSGALDKAYHETVKAVTEQIEGMKFNTAIAQLMIFVNAANKEDE
LYVAYAKGFVQLLAPFAPHLGEELWQILTASGQSISYVAWPTHDDSKLVENDVEIVVQIKGKVKAKLVVAKDLSREELEK
VALAHDKIQAEIAGKEVAKVIVVPNKLVNIVVK

Sequences:

>Translated_833_residues
MTFYNHKAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGYNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREINTTDPSYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLADLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
KDFTVFTTRPDTLFGATYAVLAPEHALVDSITTAEQAAAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEM
PIWIADYVLASYGTGAIMAVPAHDERDWAFAKQFNLEIIPVLEGGNVDEAAYTEDGIHINSGFLDGLDKACAITKMVAWL
EETGVGHEKVSYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPEQDLPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNDEQLADKDLLKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDLGVVPTKEP
FQKLFNQGMILGTSYRDHRGALVATDKVDKRDGSFFHMETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGARKFLDRVYRLITTKEIVAENSGALDKAYHETVKAVTEQIEGMKFNTAIAQLMIFVNAANKEDE
LYVAYAKGFVQLLAPFAPHLGEELWQILTASGQSISYVAWPTHDDSKLVENDVEIVVQIKGKVKAKLVVAKDLSREELEK
VALAHDKIQAEIAGKEVAKVIVVPNKLVNIVVK
>Mature_832_residues
TFYNHKAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGYNVLHPMGWDA
FGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREINTTDPSYYKWTQWIFTKLYEKGLAYEAEVPVNWVEE
LGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLADLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTDK
DFTVFTTRPDTLFGATYAVLAPEHALVDSITTAEQAAAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMP
IWIADYVLASYGTGAIMAVPAHDERDWAFAKQFNLEIIPVLEGGNVDEAAYTEDGIHINSGFLDGLDKACAITKMVAWLE
ETGVGHEKVSYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPEQDLPLVLPVTKDIRPSGTGESPLANLTDWLEVTREDG
VKGRRETNTMPQWAGSSWYYLRYIDPHNDEQLADKDLLKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPF
QKLFNQGMILGTSYRDHRGALVATDKVDKRDGSFFHMETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMG
PLDASIAWSEEGLEGARKFLDRVYRLITTKEIVAENSGALDKAYHETVKAVTEQIEGMKFNTAIAQLMIFVNAANKEDEL
YVAYAKGFVQLLAPFAPHLGEELWQILTASGQSISYVAWPTHDDSKLVENDVEIVVQIKGKVKAKLVVAKDLSREELEKV
ALAHDKIQAEIAGKEVAKVIVVPNKLVNIVVK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=886, Percent_Identity=34.3115124153499, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI94721239, Length=186, Percent_Identity=28.494623655914, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94721241, Length=186, Percent_Identity=28.494623655914, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786861, Length=866, Percent_Identity=42.6096997690531, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=391, Percent_Identity=27.1099744245524, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI2367096, Length=812, Percent_Identity=21.0591133004926, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71997510, Length=804, Percent_Identity=30.2238805970149, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71997517, Length=775, Percent_Identity=30.5806451612903, Blast_Score=348, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=29.5698924731183, Blast_Score=155, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI71980946, Length=150, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=786, Percent_Identity=36.5139949109415, Blast_Score=475, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321531, Length=382, Percent_Identity=25.6544502617801, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355409, Length=754, Percent_Identity=33.6870026525199, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17864482, Length=455, Percent_Identity=22.8571428571429, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653289, Length=455, Percent_Identity=22.8571428571429, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93737; Mature: 93606

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFYNHKAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCCHHEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRFKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREINTTDPSYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLADLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KDFTVFTTRPDTLFGATYAVLAPEHALVDSITTAEQAAAVAEYKRQASLKSDLARTDLAK
CCEEEEECCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGKEMPIWIADYVLASYGTGAIMAVPAHDERDWAFAKQFNLEIIP
HHCCCEECCEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEE
VLEGGNVDEAAYTEDGIHINSGFLDGLDKACAITKMVAWLEETGVGHEKVSYRLRDWLFS
EECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPEQDLPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNDEQLADKDLLKQWLPVDIYIGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
YARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDHRGALVATDKVDKRDGSFFHMET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCCCCCCCCEEEECC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGARKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEECHHHHHHHHHH
LDRVYRLITTKEIVAENSGALDKAYHETVKAVTEQIEGMKFNTAIAQLMIFVNAANKEDE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LYVAYAKGFVQLLAPFAPHLGEELWQILTASGQSISYVAWPTHDDSKLVENDVEIVVQIK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCEEEEEEEC
GKVKAKLVVAKDLSREELEKVALAHDKIQAEIAGKEVAKVIVVPNKLVNIVVK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHEEEECC
>Mature Secondary Structure 
TFYNHKAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCHHEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRFKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREINTTDPSYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLADLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KDFTVFTTRPDTLFGATYAVLAPEHALVDSITTAEQAAAVAEYKRQASLKSDLARTDLAK
CCEEEEECCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGKEMPIWIADYVLASYGTGAIMAVPAHDERDWAFAKQFNLEIIP
HHCCCEECCEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEE
VLEGGNVDEAAYTEDGIHINSGFLDGLDKACAITKMVAWLEETGVGHEKVSYRLRDWLFS
EECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPEQDLPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNDEQLADKDLLKQWLPVDIYIGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
YARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDHRGALVATDKVDKRDGSFFHMET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCCCCCCCCEEEECC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGARKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEECHHHHHHHHHH
LDRVYRLITTKEIVAENSGALDKAYHETVKAVTEQIEGMKFNTAIAQLMIFVNAANKEDE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LYVAYAKGFVQLLAPFAPHLGEELWQILTASGQSISYVAWPTHDDSKLVENDVEIVVQIK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCEEEEEEEC
GKVKAKLVVAKDLSREELEKVALAHDKIQAEIAGKEVAKVIVVPNKLVNIVVK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA